1887

Abstract

SUMMARY: A technique is described for mathematically normalizing whole-cell protein profiles after sodium dodecyl sulphate-polyacrylamide gel electrophoresis to obtain standardized absolute migration distances using two internal standards. A soft laser scanning densitometer was used to measure protein band migration distances in wet, silver-stained gels. The normalized values were superior to the unnormalized migration distances and common values in reducing the inter and intragel variability of the protein band positions. A procedure is described for clustering normalized bacterial protein profiles using a sample data set obtained from the type strains of four species.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-9-2653
1986-09-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/9/mic-132-9-2653.html?itemId=/content/journal/micro/10.1099/00221287-132-9-2653&mimeType=html&fmt=ahah

References

  1. Carlone G. M., Sotinek F. O., Plikaytis B. D. 1985; Comparison of outer membrane protein and biochemical profiles of Haemophilus aegyptius and Haemophilus influenzae biotype III. Journal of Clinical Microbiology 22:708–713
    [Google Scholar]
  2. Chrambach A., Jovin T. M., Svendsen P. J., Rodbard D. 1976; Analytical and preparative polyacrylamide gel electrophoresis an objectively defined fractionation route, apparatus and procedures. Methods of Protein Separation vol.2:27–144 Edited by Catsimpoolas N. New York: Plenum;
    [Google Scholar]
  3. Dice L. R. 1945; Measures of the amount of ecological association between species. Ecology 26:297–302
    [Google Scholar]
  4. Gower J. C. 1971; A general coefficient of similarity and some of its properties. Biometrics 27:857–871
    [Google Scholar]
  5. Hames B. D. 1981; An introduction to polyacrylamide gel electrophoresis. Gel Electrophoresis of Proteins: A Practical Approach Edited by Hames B. D., Rickwood D. Oxford: IRL Press; vol 2:1–91
    [Google Scholar]
  6. Izard D., Ferragut C., Gavini F., Kersters K., de Ley J., Leclerc H. 1981; Klebsiella terrigena, a new species from soil and water. International Journal of Systematic Bacteriology 31:116–127
    [Google Scholar]
  7. Jackman P. J. H. 1983; A program in BASIC for numerical taxonomy of micro-organisms based on electrophoretic band positions. Microbio Letters 23:119–124
    [Google Scholar]
  8. Jackman P. J. H. 1985; Bacterial taxonomy based on electrophoretic whole-cell protein patterns. In Chemical Methods in Bacterial Systematics pp 115–129
    [Google Scholar]
  9. Jackman P. J. H., Feltham R. K. A., Sneath P. H. A. I983; A program in BASIC for numerical taxonomy of electrophoretic protein patterns. Microbios Letters 23:87–98
    [Google Scholar]
  10. Kersters K. 1985; Numerical methods in the classification of bacteria by protein electrophoresis. In Computassisted Bacterial Systematics
    [Google Scholar]
  11. Kersters K., de Ley J. 1975; Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns. Journal of General Microbiology 87:333–342
    [Google Scholar]
  12. Kersters K., de Ley J. 1980; Classification and identification of bacteria by electrophoresis of their proteins. In Microbiological Classification and Identification. The Society for Applied Bacteriology Symposium Series, no 8:273–297
    [Google Scholar]
  13. Markwell M. A. K., Haas S. M., Bieber L. L., Tolbert N. E. 1978; A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical Biochemistry 87:206–210
    [Google Scholar]
  14. Morrissey J. H. 1981; Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Analytical Biochemistry 117:307–310
    [Google Scholar]
  15. Neville D. M., Glossman H. 1974; Molecular weight determination of membrane protein and glycoprotein subunits by discontinuous gel electrophoresis in dodecyl sulfate. Methods in Enzymology 32:92–102
    [Google Scholar]
  16. Plikaytis B. D., Carlone G. M., Edmonds P., Mayer L. W. 1986; Robust estimation of standard curves for protein molecular weight and linear-duplex DNA base pair number after gel electrophoresis. Analytical Biochemistry 152:346–364
    [Google Scholar]
  17. Rosner B. 1982; Fundamentals of Biostatistics, pp Boston, Mass. Duxbury Press26–27
    [Google Scholar]
  18. Seiter J. A., Jay J. M. 1980; Application of polyacrylamide gel electrophoresis to the characterization and identification of Arthrobacter species. International Journal of Systemaitc Bacteriology 30:460–465
    [Google Scholar]
  19. Swings J., Kersters K., de Ley J. 1976; Numerical analysis of electrophoretic protein patterns of Zymomonas strains. Journal of General Microbiology 93:266–271
    [Google Scholar]
  20. Tsang V. C. W., Peralta J. M., Simons A. R. 1983; Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods in Enzymology 92:377–391
    [Google Scholar]
  21. Vera Cruz C. M., Gossele F., Kersters K., Segers P., van den Mooter M., Swings J., de Ley J. 1984; Differentiation between Xanthomonas campestris pv oryzae, Xanthomonas campestris pv. oryzicola and the bacterial 'brown blotch' pathogen on rice by numerical analysis of phenotypic features and protein gel electrophoregrams. Journal of General Microbiology 130:2983–2999
    [Google Scholar]
  22. Wishart D. 1978 Clustan User Manual, version 1C, release 2, 3rd edn. Edinburgh: Program Library Unit, Edinburgh University, UK;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-9-2653
Loading
/content/journal/micro/10.1099/00221287-132-9-2653
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error