1887

Abstract

SUMMARY: Strains of of serotype K21 are frequently involved in outbreaks of nosocomial infections. The type strain of K21 (which we have renamed K21a) produces capsular polysaccharide that contains mannose, galactose and glucuronic acid in the ratio 2:2:1. In contrast, all eight of the randomly chosen isolates of that were initially typed as K21 were shown by paper chromatography and NMR spectroscopy to produce a different capsular polysaccharide. We have designated this new polysaccharide K21b. The K21b capsular material appears to have a closely similar immunodominant side chain to K21a. However, K21b polysaccharide has two molecules of rhamnose in the polysaccharide backbone replacing the two molecules of mannose found in the K21a capsule. Our results suggest that the K21b serotype may be more frequently distributed than the classical K21a type.

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/content/journal/micro/10.1099/00221287-133-5-1365
1987-05-01
2024-04-26
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References

  1. Anonymous 1974; Dyeing reagents for thin layer chromatography. Darmstadt:: E.Merck;
    [Google Scholar]
  2. Barr J. G. 1981; Spontaneous loss and transfer of plasmid-mediated K21 antigen synthesis in Klebsiella pneumoniae. Journal of Clinical Pathology 34:434–438
    [Google Scholar]
  3. Bock K., Thøgersen H. 1982; Nuclear magnetic resonance spectroscopy in the study of mono-and oligosaccharides. Annual Reports on NMR Spectroscopy 13:1–57
    [Google Scholar]
  4. Casewell M. W., Talsania H. G. 1979; Predominance of certain Klebsiella capsular types in hospitals in the United Kingdom. Journalof Injection 1:77–79
    [Google Scholar]
  5. Choy Y. M., Dutton G. G. S. 1973; The structure of the capsular polysaccharide from Klebsiella K-type 21. Canadian Journal of Chemistry 51:198–207
    [Google Scholar]
  6. De bruyn A., Anteunis M., De gussem R., Dutton G. G. S. 1976; 1H N.M.R. study of l-rhamnose, methyl α-l-rhamnopyranoside, and 4-Oβ-d-galactopyranosyl-l-rhamnose in deuterium oxide. Carbohydrate Research 47:158–163
    [Google Scholar]
  7. Dutton G. G. S., Lim A. V. S. 1985; Structure of the capsular polysaccharide of Klebsiella serotype K79. Carbohydrate Research 144:263–276
    [Google Scholar]
  8. Gotschlich E. C., Liu T. Y., Artenstein M. S. 1969; Human immunity to the meningococcus. III. Preparation and immunochemical properties of the group A, group B and group C meningococcal polysaccharides. Journal of Experimental Medicine 129:1349–1356
    [Google Scholar]
  9. Luderitz O., Staub A. M. 1966; Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriological Reviews 30:192–255
    [Google Scholar]
  10. Meers P. D., Ayliffe G. A. J., Emmerson A. M., Leigh D. A., Mayon-White R. T., Mackingtosh C. A., Strange J. L. 1981; National Survey of Infection in Hospitals. Journal of Hospital Infections 2: (supplement) 1–39
    [Google Scholar]
  11. Nimmich W. 1968; Zur Isolierung und qualitativen Bausteinanalyse der K-antigene von Klebsiellen. Zeitschrift fur medizinische Mikrobiologie und Im-munologie 154:117–131
    [Google Scholar]
  12. Pritchard D. G., Furner R. L. 1985; Structure of the group specific polysaccharide of group E Streptococcus. Carbohydrate Research 144:289–296
    [Google Scholar]
  13. Sutherland I. W. 1977; Bacterial exopolysaccharides -their nature and production. In Surface Carbohydrates of the Prokaryotic Cell pp. 27–96 Sutherland I. W. Edited by London & New York: Academic Press;
    [Google Scholar]
  14. Vliegenhart J. F. G., Dorland L., Vanhalbeck H. 1983; High resolution, 1H n.m.r. spectroscopy as a tool in the structural analysis of carbohydrates related to glycoproteins. Advances in Carbohydrate Chemistry and Biochemistry 41:221–225
    [Google Scholar]
  15. Wilkinson J. F., Dudman W. F., Aspinall G. D. 1955; The extracellular polysaccharide of Aero-bacter aerogenes A3 (SI) (Klebsiella Type 54). Biochemical Journal 59:446–451
    [Google Scholar]
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