1887

Abstract

A 6·1 kb DNA probe for the human enteric pathogen has been isolated from a genomic library constructed in the plasmid vector pBR322 in . The DNA sequence used as a probe was identified from recombinant plasmids following immunological screening of transformants using polyclonal antisera to whole cells and to membrane antigens of . Restriction endonuclease fragment mapping of DNA inserts from three of the recombinant plasmids showed an overlapping DNA fragment. One of these recombinant plasmids, when used as a DNA probe in Southern hybridization, specifically hybridized with chromosomal DNA from all of the strains tested. Hybridization was not detected at high stringency between the DNA probe and chromosomal DNA from any other species tested except weakly with the chromosomal DNA of strains of . Hybridization was also not detected with chromosomal DNA from a range of other enteric bacteria likely to be encountered in faecal material. The intensity of hybridization with could be increased by reducing the stringency of hybridization.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-2-521
1988-02-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/2/mic-134-2-521.html?itemId=/content/journal/micro/10.1099/00221287-134-2-521&mimeType=html&fmt=ahah

References

  1. Bachmann B.J. 1983; Linkage map of Escherichia coli. Edition 7. Microbiological Reviews 47:180–230
    [Google Scholar]
  2. Blaser M.J., Reller L.B. 1981; Campylobacter enteritis. New England Journal of Medicine 305:1444–1452
    [Google Scholar]
  3. Blaser M.J., Hopkins J.A., Berka R.M., Vasil M.L., Wang W.L. 1983; Identification and characterization of Campylobacter jejuni outer membrane proteins. Infection and Immunity 42:276–284
    [Google Scholar]
  4. Bolivar F., Rodriguez R.C., Greene P.J., Betlach M.C., Heynecker H.C., Boyer H.W., Crosa J.H., Falkow S. 1977; Construction and characterization of new cloning vehicles II.A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  5. Bradbury W.C., Pearson A.D., Marko M.A., Congi R.V., Penner J.L. 1984; Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis. Journal of Clinical Microbiology 19:343–346
    [Google Scholar]
  6. Claus P., Moseley S.L., Falkow S. 1982; The use of gene-specific DNA probes for the identification of enteric pathogens. Progress in Food and Nutrition Science 7:139–142
    [Google Scholar]
  7. Collins D.M., Ross D.E. 1984; Restriction endonuclease analysis of Campylobacter strains with particular reference to Campylobacter fetus ss. fetus. Journal of Medical Microbiology 18:117–124
    [Google Scholar]
  8. Ferguson D.A., Lambe D.W. 1984; Differentiation of Campylobacter species by protein banding patterns in polyacrylamide slab gels. Journal of Clinical Microbiology 20:453–460
    [Google Scholar]
  9. Frey J., Bagdasarian M., Feiss D., Franklin F.C.H., Deshusses J. 1983; Stable cosmid vectors that enable the introduction of cloned fragments into a wide range of Gram-negative bacteria. Gene 24:299–308
    [Google Scholar]
  10. Grimont P.A.D., Grimont F., Desplaces N., Tchen P. 1985; DNA probe specific for Legionella pneumophila. Journal of Clinical Microbiology 21:431–437
    [Google Scholar]
  11. Hébert G.A., Hollis D.G., Weaver R.E., Lambert M.A., Blaser M.J., Moss C.W. 1982; 30 years of Campylobacters, biochemical characteristics and a biotyping proposal for Campylobacter jejuni. Journal of Clinical Microbiology 15:1065–1073
    [Google Scholar]
  12. Hébert G.A., Edmonds P., Brenner D.J. 1984; DNA relatedness among strains of Campylobacter jejuni and Campylobacter coli with divergent sero-group and hippurate reactions. Journal of Clinical Microbiology 20:138–140
    [Google Scholar]
  13. Krishnapillai V., Nash J., Lanka E. 1984; Insertion mutations in the promiscuous IncP-1 plasmid R18 which affect its host range between Pseudomonas species. Plasmid 12:170–180
    [Google Scholar]
  14. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Logan S.M., Trust T.J. 1982; Outer membrane characteristics of Campylobacter jejuni. Infection and Immunity 38:898–906
    [Google Scholar]
  16. Logan S.M., Trust T.J. 1983; Molecular identification of surface protein antigens of Campylobacter jejuni. Infection and Immunity 42:675–682
    [Google Scholar]
  17. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning, A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Mills D.S., Bradbury W.C. 1984; Human antibody response to outer membrane proteins of Campylobacter jejuni during infection. Infection and Immunity 43:739–743
    [Google Scholar]
  19. Owen R.J., Dawson C. 1986; DNA base compositions and base sequence relatedness of atypical Campylobacter jejuni strains from clinical material. FEMS Microbiology Letters 35:283–287
    [Google Scholar]
  20. Penner J.L., Hennessy J.N., Congi R.V. 1983; Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. European Journal of Clinical Microbiology 2:278–283
    [Google Scholar]
  21. Rashtchian A., Abbott M., Lovern D.H., Mock G.A., Shaffer M.M. 1986; Use of synthetic oligonucleotides complementary to rRNA for detection of Campylobacter. Abstracts of the Annual Meeting of the American Society for Microbiology abstract no. C-90 p. 343
    [Google Scholar]
  22. Rubin F.A., Kopecko D.J., Noon K.F., Baron L.S. 1985; Development of a DNA probe to detect Salmonella typhi. Journal of Clinical Microbiology 22:600–605
    [Google Scholar]
  23. Skirrow M.B. 1982; Campylobacter enteritis - the first five years. Journal of Hygiene 89:175–184
    [Google Scholar]
  24. Skirrow M.B., Benjamin J., Razi M.H.H., Waterman S. 1982; Isolation, cultivation and identification of Campylobacter jejuni and C.coli. In Isolation and Identification Methods for Food Poisoning Organisms, Society for Applied Bacteriology Technical Series no. 17 pp. 313–323 Edited by Corry J. E. L., Roberts D., Skinner F. A. London: Academic Press;
    [Google Scholar]
  25. Smith G.E., Summers M.D. 1980; The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. Analytical Biochemistry 109:123–129
    [Google Scholar]
  26. Steele T.W., Sangster N., Lanser J.A. 1985; DNA relatedness and biochemical features of Campylobacter spp. isolated in Central and South Australia. Journal of Clinical Microbiology 22:71–74
    [Google Scholar]
  27. Tauxe R.V., Patton C.M., Edmonds P., Barrett T.J., Brenner D.J., Blake P.A. 1985; Illness associated with Campylobacter laridis, a newly recognized Campylobacter species. Journal of Clinical Microbiology 21:222–225
    [Google Scholar]
  28. Tompkins L.S., Troup N., Labigne-Rousel A., Cohen M.L. 1986; Cloned, random chromosomal sequences as probes to identify Salmonella species. Journal of Infectious Diseases 154:156–162
    [Google Scholar]
  29. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets, procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-2-521
Loading
/content/journal/micro/10.1099/00221287-134-2-521
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error