1887

Abstract

An LH2 strain of , DBC1, has been constructed by deleting the operon, which encodes the LH2 α and polypeptides, from the chromosome and replacing it with a kanamycin resistance gene. Southern blot analysis indicates that the 950 bp HI restriction fragment which contains the operon has been lost and has been replaced by the 1·25 kb Km cassette derived from Tn Strain DBC1 lacked the LH2 complex, as shown by loss of the characteristic absorbance bands at 800 and 850 nm. The LH2 polypeptides were also found to be absent after SDS-PAGE. The wild-type phenotype was restored to DBC1 by the transfer of a 3·8 kb I fragment containing the operon in plasmid pMA81. Transconjugants possessed a wild-type absorbance spectrum and LH2 polypeptides.

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1989-07-01
2024-05-04
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References

  1. Aagaard J., Sistrom W.R. 1972; Control of synthesis of reaction center bacteriochlorophyll in photosynthetic bacteria. Photochemistry and Photobiology 15:209–225
    [Google Scholar]
  2. Ashby M.K., Coomber S.A., Hunter C.N. 1987; Cloning, nucleotide sequence and transfer of genes for the B800850 light harvesting complex of Rhodobacter sphaeroides. FEBS Letters 213:245–248
    [Google Scholar]
  3. Broglie R.M., Hunter C.N., Delepelaire P., Niederman R.A., Chua N.H., Clayton R.K. 1979; Isolation and characterisation of the pigment protein complexes of Rhodopseudomonas sphaeroidesby lithium dodecyl sulphate polyacrylamide gel electrophoresis. Proceedings of the National Academy of Sciences of the United States of America 77:87–91
    [Google Scholar]
  4. Brunisholz R.A., Zuber H. 1988; Primary structure analysis of bacterial antenna polypeptides. In Photosynthetic Light Harvesting Systems: Organisation and Function,, 1st. pp 103–114 Scheer H., Schneider S. Edited by Berlin & New York: Walter de Gruyter;
    [Google Scholar]
  5. Donohue TJ., Hoger J.H., Kaplan S. 1986; Cloning and expression of the Rhodobacter sphaeroides reaction centre H gene. Journal of Bacteriology 168:953–961
    [Google Scholar]
  6. VanDorssen R.J., Hunter C.N., VanGrondelle R., Korenhof A.H., Amesz J. 1988; Spectroscopic properties of antenna complexes of Rhodobacter sphaeroides in vivo. Biochimica et biophysica acta 932:179–188
    [Google Scholar]
  7. Feinberg A.P., Vogelstein B. 1983; A technique for radiolabelling DNA restriction fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  8. Hanahan D. 1985; Techniques for the transformation of E. coli. . In DNA Cloning, 1, 1st. pp. 109–135 Glover D. M. Edited by Oxford: IRL Press;
    [Google Scholar]
  9. Hunter C.N., Turner G. 1988; Transfer of genes coding for apoproteins of reaction centre and lightharvesting LH1 complexes to Rhodobacter sphaeroides. Journal of General Microbiology 134:1471–1480
    [Google Scholar]
  10. Hunter C.N., VanGrondelle R. 1988; The use of mutants to investigate the organisation of the photosynthetic apparatus of Rhodobacter sphaeroides. In Structure and Function in Photosynthetic Light Harvesting Systems, pp 247–260 Scheer H., Schneider S. Edited by Berlin: Walter De Gruyter;
    [Google Scholar]
  11. Hunter C.N., VanGrondelle R., Olsen J.D. 1989; Photosynthetic antenna proteins: lOOps before photochemistry starts. Trends in Biochemical Sciences 14:72–76
    [Google Scholar]
  12. Kiley P.J., Kaplan S. 1987; Cloning, DNA sequence, and expression of the Rhodobacter sphaeroides light-harvesting B800-850α and B800-850β-genes. Journal of Bacteriology 169:3268–3275
    [Google Scholar]
  13. Kiley P.J., Kaplan S. 1988; Molecular genetics of photosynthetic membrane biosynthesis in Rhodobacter sphaeroides. Microbiological Reviews 52:50–69
    [Google Scholar]
  14. Kiley P.J., Donohue T.J., Havelka W.A., Kaplan S. 1987; DNA sequence and in vitro expression of the B875 light-harvesting polypeptides of Rhodobacter sphaeroides. Journal of Bacteriology 169:742–750
    [Google Scholar]
  15. Kramer H.J.M., VanGrondelle R., Hunter C.N., Westerhuis W.H.J., Amesz J. 1984; Pigment organisation of the B800-850 antenna complex of Rhodopseudomonas sphaeroides. Biochimica et biophysica acta 765:156–165
    [Google Scholar]
  16. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Messing J., Grea R., Seeburg P.H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  18. Niaudet B., Goze A., Ehrlich S.D. 1982; Insertional mutagenesis in Bacillus subtilis: mechanism and use in gene cloning. Gene 19:277–284
    [Google Scholar]
  19. Niederman R.A., Mallon D.E., Langan J.J. 1976; Membranes of Rhodopseudomonas sphaer-oides. IV. Assembly of chromatophores in low aeration cell suspensions. Biochimica et biophysica acta 440:429–447
    [Google Scholar]
  20. Sanger F., Nicklen S., Coulsen A.R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 80:6505–6509
    [Google Scholar]
  21. Schumacher A., Drews G. 1979; The effects of light intensity on membrane differentiation in Rhodopseudomonas capsulata. Biochimica et biophysica acta 547:417–428
    [Google Scholar]
  22. Simon R., Priefer U., Puhler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gramnegative bacteria. Bio/Technology 1:784–791
    [Google Scholar]
  23. Tadros M.H., Zuber H., Drews G. 1982; The polypeptide components from the light-harvesting pigment-protein complex II (B800-850) of Rhodopseudomonas capsulata. European Journal of Biochemistry 127:315–318
    [Google Scholar]
  24. Theiler R., Suter F., Zuber H., Cogdell R.J. 1984; A comparison of the primary structures of the two B800-850-apoproteins from wild-type Rhodopseudomonas sphaeroides strain 2.4.1 and a carotenoid-less mutant strain R26.1. FEBS Letters 175:231–237
    [Google Scholar]
  25. Theiler R., Suter F., Pennoyer J.D., Zuber H., Niederman R.A. 1985; Complete amino acid sequence of the B875 light-harvesting protein of Rhodopseudomonas sphaeroides strain 2.4.1. FEBS Letters 184:231–236
    [Google Scholar]
  26. Vos M., vangrondelle R., vandeKooij F.W., vandePoll D., Amesz J., Duysens L.N.M. 1986; Singlet-singlet annihilation at low temperatures in the antenna of purple bacteria. Biochimica et biophysica acta 850:501–512
    [Google Scholar]
  27. Vos M., vanDorssen R.J., Amesz J., vanGrondelle R., Hunter C.N. 1988; The organisation of the photosynthetic apparatus of Rhodobacter sphaeroides: studies of antenna mutants using singlet-singlet quenching. Biochimica et biophysica acta 933:132–140
    [Google Scholar]
  28. Williams J.C., Steiner L.A., Ogden R.C., Simon M.I., Feher G. 1983; Primary structure of the M subunit of the reaction centre from Rhodopseudomonas sphaeroides. Proceedings of the National Academy of Sciences of the United States of America 80:6505–6509
    [Google Scholar]
  29. Williams J.C., Steiner L.A., Feher G., Simon M.I. 1984; Primary structure of the L subunit of the reaction centre from Rhodopseudomonas sphaeroides. Proceedings of the National Academy of Sciences of the United States of America 81:7303–7308
    [Google Scholar]
  30. Woods D. 1984; Oligonucleotide screening of cDNA Libraries. Focus (Bethesda Research Laboratories) 6(3):1–3
    [Google Scholar]
  31. Youvan D.C., Ismail S., Bylina E.J. 1985; Chromosomal deletion and plasmid complementation of the photosynthetic reaction centre and light harvesting genes from Rhodopseudomonas sphaeroides. Gene 38:19–30
    [Google Scholar]
  32. Zuber H. 1986; Structure of the light-harvesting antenna complexes of photosynthetic bacteria, cyanobacteria and red algae. Trends in Biochemical Sciences 11:414–419
    [Google Scholar]
  33. Zucconi A.P., Beatty J.T. 1988; Posttranscriptional regulation by light of the steady-state levels of mature B800-850 light-harvesting complexes in Rhodobacter capsulatus. Journal of Bacteriology 170:877–882
    [Google Scholar]
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