1887

Abstract

By using competition between glucose and its analogue -glucosamine, we have produced a system in which it is possible to vary the steady-state growth rate of populations of cells without otherwise altering the composition of the medium or significantly affecting catabolite repression. We demonstrate that -glucosamine inhibits the accumulation of glucose derived label and the phosphorylation of glucose by hexokinase (EC 2.7.1.1).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-9-2407
1989-09-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/9/mic-135-9-2407.html?itemId=/content/journal/micro/10.1099/00221287-135-9-2407&mimeType=html&fmt=ahah

References

  1. Bailey R.G., Woodward P. 1984; Isolation and characterisation of pleiotropic glucose repression resistant mutant of Saccharomyces cerevisiae.. Molecular and General Genetics 193:507–512
    [Google Scholar]
  2. Beck C., Von Meyenburg H.K. 1968; Enzyme pattern and aerobic growth of Saccharomyces cerevisiae under various degrees of glucose limitation.. Journal of Bacteriology 96:479–486
    [Google Scholar]
  3. Bisson L. 1988; High-affinity glucose transport in Saccharomyces cerevisiae is under general glucose repression control.. Journal of Bacteriology 170:4838–4845
    [Google Scholar]
  4. Bisson L., Fraenkel D.G. 1983a; Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae.. Proceedings of the National Academy of Sciences of the United States of America 80:1730–1734
    [Google Scholar]
  5. Bisson L., Fraenkel D.G. 1983b; Transport of 6-deoxyglucose in Saccharomyces cerevisiae.. Journal of Bacteriology 155:995–1000
    [Google Scholar]
  6. Bisson L., Fraenkel D.G. 1984; Expression of kinase dependent glucose uptake in Saccharomyces cerevisiae.. Journal of Bacteriology 159:1013–1017
    [Google Scholar]
  7. Burger M., Hejmova J. 1961; Uptake of metabolisable sugars by Saccharomyces cerevisiae.. Folia microbiologica 6:80–85
    [Google Scholar]
  8. Dixon M. 1953; The determination of enzyme inhibitor constants.. Biochemical Journal 55:170–171
    [Google Scholar]
  9. Dodyk F., Rothstein A. 1964; Factors influencing the appearance of invertase in Saccharomyces cerevisiae.. Archives of Biochemistry and Biophysics 104:478–486
    [Google Scholar]
  10. Entian K.D., Kopetzki E., Frohlich K.U., Mecke D. 1984; Cloning of hexokinase isoenzyme PI from Saccharomyces cerevisiae : PI transformants confirm the unique role of hexokinase isoenzyme PII for glucose repression in yeasts.. Molecular and General Genetics 198:50–54
    [Google Scholar]
  11. Furst A., Michels C.A. 1977; An evaluation of d-glucosamine as a gratuitous catabolite repressor of Saccharomyces cerevisiae.. Molecular and General Genetics 155:309–314
    [Google Scholar]
  12. Hockney R.C., Freeman R.F. 1980; Gratuitous catabolite repression by glucosamine of maltose utilization in Saccharomyces cerevisiae.. Journal of General Microbioloev 121:479–482
    [Google Scholar]
  13. Lang J.M., Cirillo V.P. 1987; Glucose transport in kinaseless Saccharomyces cerevisiae mutant.. Journal of Bacteriology 169:2932–2937
    [Google Scholar]
  14. Lobo Z., Maitra P.K. 1977; Resistance of 2-deoxyglucose in yeast: a direct selection of mutants lacking glucose phosphorylating enzymes.. Molecular and General Genetics 157:297–300
    [Google Scholar]
  15. Lineweaver H., Burk D. 1934; Determination of enzyme dissociation constants.. Journal of the American Chemical Society 56:658–666
    [Google Scholar]
  16. Magasanik B. 1961; Catabolite repression.. Cold Spring Harbor Symposia on Quantitative Biology 26:249–256
    [Google Scholar]
  17. Matsumoto K., Uno I., Ishikawa T., Oshima Y. 1983; Cyclic AMP may be involved in catabolite repression in Saccharomyces cerevisiae: evidence from mutants unable to synthesize it.. Journal of Bacteriology 156:898–900
    [Google Scholar]
  18. Michels C.A., Romanski A. 1980; Pleiotropic glucose repression resistant mutants in Saccharomyces carlsbergertsis.. Journal of Bacteriology 143:674–679
    [Google Scholar]
  19. Miller G.L. 1959; Use of dinitrosalicylic acid reagent for detection of reducing sugar.. Analytical Chemistry 31:426–428
    [Google Scholar]
  20. Pirt S.J. 1971; The diffusion capsule.. Biochemical Journal 121:293–297
    [Google Scholar]
  21. Pirt S.J. 1975 Principles of Microbe and Cell Cultivation. Oxford: Blackwells;
    [Google Scholar]
  22. Polakis E.S., Bartley W. 1965; Changes in the enzyme activity of Saccharomyces cerevisiae during growth on different carbon sources.. Biochemical Journal 97:284–297
    [Google Scholar]
  23. Ramos J., Szkutnicka K., Cirillo V.P. 1988; Relationship between low- and high-affinity glucose transport systems of Saccharomyces cerevisiae.. Journal of Bacteriology 170:5375–5377
    [Google Scholar]
  24. Woodward G.E., Hudson M.T. 1953; d-Glucosamine as an antagonist of glucose in carbohydrate metabolism of yeast.. Journal of the Franklin Institute 255:556–560
    [Google Scholar]
  25. Woodward G.E., Cramer F.B., Hudson M.T. 1953; Carbohydrate analogues as antagonists of glucose in carbohydrate metabolism of yeast.. Journal of the Franklin Institute 256:577–587
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-9-2407
Loading
/content/journal/micro/10.1099/00221287-135-9-2407
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error