1887

Abstract

synthesized only -type and -type cytochromes under the wide range of growth conditions tested, and reaction with CO revealed two potential oxidases. The -type oxidase was produced only in the presence of O and appeared to be repressed by glucose. The -type oxidase was, by contrast, produced only in the absence of measurable O (< 1μ), and was the only oxidase expressed in nitrogen-fixing conditions. It was extracted from the membrane, purified and shown to be similar to that from in being a heterodimer (subunits of 52000 and 35000), in containing two distinguishable haems and haem (one or two molecules per molecule of oxidase), and in being able to react with O to give a stableoxygenated intermediate. The purified -type cytochrome oxidase had a very high affinity for O ( 20 n; measured by the spectral properties of leghaemoglobin). It is proposed that this provides a role for this oxidase in lowering the O concentration to allow nitrogenase synthesis and function, and to provide a terminal oxidase to permit electron-transport-coupled ATP synthesis which supports the increase in efficiency of nitrogen fixation observed under microaerobic conditions.

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1990-01-01
2024-05-02
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References

  1. Ames G. F. 1968; Lipids of Salmonella typhimurium and Escherichia coli : structure and metabolism. Journal of Bacteriology 95:833–843
    [Google Scholar]
  2. Anraku Y., Gennis R. B. 1987; The aerobic respiratory chain of Escherichia coli. Trends in Biochemical Sciences 139:262–266
    [Google Scholar]
  3. Appleby C. A., Bergersen F. J. 1980; Preparation and the experimental use of leghaemoglobin. In Methods for Evaluating Biological Nitrogen Fixation, pp 315–335 Bergersen F. J. Edited by Chichester: J. Wiley;
    [Google Scholar]
  4. Au D.C.T., Lorence R. M., Gennis R. B. 1985; Isolation and characterization of an Escherichia coli mutant lacking the cytochrome o terminal oxidase. Journal of Bacteriology 161:123–127
    [Google Scholar]
  5. Barrett J. 1956; The prosthetic group of cytochrome a2 . Biochemical Journal 64:626–639
    [Google Scholar]
  6. Bergersen F. J., Turner G. L. 1979; Systems utilising oxygenated leghaemoglobin and myoglobin as sources of free dissolved oxygen at low concentrations for experiments with bacteria. Analytical Biochemistry 96:165–174
    [Google Scholar]
  7. Bergersen F. J., Kennedy C., Hill S. 1982; The influence of low oxygen concentrations on derepression of nitrogenase in Klebsiella pneumoniae. Journal of General Microbiology 128:909–915
    [Google Scholar]
  8. Bligh E. G., Dyer W. J. 1959; A rapid method for total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  9. Cannon F. C. 1980; Genetic studies with diazotrophs. In Methodsfor Evaluating Nitrogen Fixation, pp 367–413 Bergersen F. J. Edited by Chichester: J. Wiley;
    [Google Scholar]
  10. Finlayson S. D., Ingledew W. J. 1985; Cytochrome bd of Escherichia coli: its isolation and study by EPR. Biochemical Society Transactions 13:632–633
    [Google Scholar]
  11. Furhop J. H., Smith K. M. 1975; Laboratory methods. In Porphyrins and Metalloporphyrins, pp 757–869 Smith K. M. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  12. Georgiou C. D., Dueweke T. J., Gennis R. B. 1988; Regulation of expression of the cytochrome d terminal oxidase in Escherichia coli is transcriptional. Journal of Bacteriology 170:961–966
    [Google Scholar]
  13. Green G. N., Gennis R. B. 1983; Isolation acharacterization of an Escherichia coli mutant lacking cytochrome d terminal oxidase. Journal of Bacteriology 154:1269–1275
    [Google Scholar]
  14. Harrison D.E.F. 1972; Physiological effects of dissolved oxygen potential and redox potential on growing populations of microorganisms. Journal of Applied Chemistry and Biotechnology 22:417–446
    [Google Scholar]
  15. Hendrich J. L., Smith A. J. 1978; Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis. Archives of Biochemistry and Biophysics1 1126:155–164
    [Google Scholar]
  16. Hill S. 1976; Influence of atmospheric oxygen concentration on acetylene reduction and efficiency of nitrogen fixation in intact Klebsiella pneumoniae. Journal of General Microbiology 93:335–345
    [Google Scholar]
  17. Hill S. 1988; How is nitrogenase regulated by oxygen?. FEMS Microbiology Reviews 54:111–130
    [Google Scholar]
  18. Hill S., Turner G. L., Bergersen F. J. 1984; Synthesis and activity of nitrogenase in Klebsiella pneumoniae exposed to low concentrations of oxygen. Journal of General Microbiology 130:1061–1067
    [Google Scholar]
  19. Ingledew W. J., Poole R. K. 1984; The respiratory chains of Escherichia coli. Microbiological Revues 48:222–271
    [Google Scholar]
  20. Kita K., Konishi K., Anraku Y. 1984a; Terminal oxidases of the Escherichia coli aerobic respiratory chain: purification and properties of cytochrome b562o complex from cells in early exponential phase of aerobic growth. Journal of Biological Chemistry 259:3368–3374
    [Google Scholar]
  21. Kita K., Konishi K., Anraku Y. 1984b; Terminal oxidases of Escherichia coli respiratory chain. Purification and properties of the cytochrome b-558d complex from cells grown with limited oxygen, and evidence of a branched electron carrying system. Journal of Biological Chemistry 259:3375–3381
    [Google Scholar]
  22. Kranz G. R., Gennis R. B. 1985; Immunological investigation of the distribution of cytochromes related to the two terminal oxidases of Escherichia coli in other Gram-negative bacteria. Journal of Bacteriology 161:709–713
    [Google Scholar]
  23. Kranz R. G., Barassi C. J., Gennis R. B. 1984; Immunological analysis of the heme proteins present in aerobically-grown Escherichia coli. Journal of Bacteriology 158:1191–1194
    [Google Scholar]
  24. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  25. Lorence R. M., Koland J. G., Gennis R. B. 1986; Coulometric and spectroscopic analysis of the purified cytochrome d oxidase complex of Escherichia coli: evidence for the identification of ‘cytochrome a,’ as cytochrome b595 . Biochemistry 25:2314–2321
    [Google Scholar]
  26. Miller M. J., Gennis R. B. 1983; Purification and characterisation of the cytochrome d terminal oxidase complex from Escherichia coli. Journal of Biological Chemistry 248:9159–9165
    [Google Scholar]
  27. Miller M. J., Hermodson M., Gennis R. B. 1988; The active form of the cytochrome d terminal oxidase complex of Escherichia coli is a heterodimer containing one copy of each of the two subunits. Journal of Biological Chemistry 263:5235–5240
    [Google Scholar]
  28. Moss F. 1956; Adaptation of the cytochromes of Aerobacter aerogenes in response to environmental oxygen tension. Australian Journal of Experimental Biology 34:395–406
    [Google Scholar]
  29. Poole R. K. 1988; Bacterial cytochrome oxidases. In Bacterial Energy Transduction, pp 231–291 Anthony C. Edited by London: Academic Press;
    [Google Scholar]
  30. Poole R. K., Waring A. J., Chance B. 1979; The reaction of cytochrome o in Escherichia coli with oxygen. Biochemical Journal 184:379–389
    [Google Scholar]
  31. Poole R. K., Kumar C., Salmon I., Chance B. 1983; The 650 nm chromophore in Escherichia coli is an oxygenated compound, not the oxidized form of cytochrome d oxidase; an hypothesis. Journal of General Microbiology 129:1335–1344
    [Google Scholar]
  32. Rice C. W., Hempfling W. P. 1978; Oxygen-limited continuous culture and respiratory enzymes in Escherichia coli. Journal of Bacteriology 134:115–124
    [Google Scholar]
  33. Rothery R. A., Ingledew W. J. 1989; The cytochromes of anaerobically-grown Escherichia coli: an electron paramagnetic resonance study of the in situ cytochrome bd complex. Biochemical Journal 261:437–443
    [Google Scholar]
  34. Smith A., Hill S., Anthony C. 1988; A haemoprotein is not involved in the control by oxygen of enteric nitrogenase synthesis. Journal of General Microbiology 134:1499–1507
    [Google Scholar]
  35. Yates M. G. 1988; The role of oxygen and hydrogen in nitrogen fixation. Symposia of the Society for General Microbiology 42:383–416
    [Google Scholar]
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