1887

Abstract

Thermotolerance in is induced by exposing cells to a brief heat shock (42 °C for 15 min). This results in resistance to the lethal effect of exposure to a higher temperature (50 °C). Mutants defective in the and genes are more sensitive to heat than the wild-type. However, after development of thermotolerance these mutants are like the wild-type in their heat sensitivity. This suggests that thermotolerance is an inducible response capable of protecting cells from the lethal effects of heat, independently of and Thermotolerance does not develop in a mutant. In addition, the mutant is sensitive to heat and HO, but is resistant to UV irradiation. This implies that the heat-shock response includes a mechanism that protects cells from heat and HO, but not from UV.

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1990-10-01
2024-04-30
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References

  1. Adams M.H. 1959 Bacteriophages. New York: : Interscience;
    [Google Scholar]
  2. Ames B., Saul N., Schwiers E., Adelman R., Cathcart R. 1985; Oxidative DNA damage as related to cancer and aging: assay of thymine glycol, thymidine glycol, and hydroxymethyluracil in human and rat urine. In Molecular Biology of Aging: Gene Stability and Gene Expression p. 137 Sohoal R.S. Edited by New York: Raven Press;
    [Google Scholar]
  3. Ananthaswamy H.N., Eisenstark A. 1977; Repair of hydrogen peroxide-induced single strand breaks in Escherichia coli deoxyribonucleic acid. Journal of Bacteriology 130:187–191
    [Google Scholar]
  4. Brandi G., Sestili P., Pedrini M.A., Salvaggio L., Cattabeni F., Cantoni O. 1987; The effect of temperature or anoxia on Escherichia coli killing induced by hydrogen peroxide. Mutation Research 190:237–240
    [Google Scholar]
  5. Bridges B.A., Ashwood-smith M.J., Munson R.J. 1969a; Correlation of bacterial sensitivities to ionizing radiation and mild heating. Journal of General Microbiology 58:115–124
    [Google Scholar]
  6. Bridges B.A., Ashwood-smith M.J., Munson R.J. 1969b; Susceptibility of mild thermal and of ionizing radiation damage to the same recovery mechanisms in Escherichia coli. Biochemical and Biophysical Research Communications 35:193–196
    [Google Scholar]
  7. Bukau B., walker G.C. 1989; Cellular defects caused by deletion of the Escherichia coli dnaK gene indicate roles for heat shock proteins in normal metabolism. Journal of Bacteriology 171:2337–2346
    [Google Scholar]
  8. Demple B., Halbrock J., Linn S. 1983; Escherichia coli xth mutants are hypersensitive to hydrogen peroxide. Journal of Bacteriology 153:1079–1082
    [Google Scholar]
  9. Demple B., Johnson A., Fung D. 1986; Exonuclease III and endonuclease IV remove 3ʹ blocks from DNA synthesis primers in H2O2 damaged Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 83:7731–7735
    [Google Scholar]
  10. Dodson M., Echols H., Wickner S., Alfano C., Mensa-Wilmot K., Gomes H., LeBowitz J., Roberts J.D., McMacken R. 1987; Specialized nucleo-protein structures at the origin of replication of bacteriophage λ: localized unwinding of duplex DNA by a six-protein reaction. Proceedings of the National Academy of Sciences of the United States of America 83:7638–7642
    [Google Scholar]
  11. Hagensee M.E., Moses R.E. 1986; Repair response of Escherichia coli to hydrogen peroxide DNA damage. Journal of Bacteriology 168:1059–1065
    [Google Scholar]
  12. Howard-flanders P., Theriot L. 1966; Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics 53:1137–1150
    [Google Scholar]
  13. Howard-Flanders P., Theriot L., Stedford J.B. 1969; Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12. Journal of Bacteriology 97:1134–1141
    [Google Scholar]
  14. Itikawa H., Ryu J.-I. 1979; Isolation and characterization of a temperature-sensitive dnaK mutant of Escherichia coli B. Journal of Bacteriology 138:339–344
    [Google Scholar]
  15. Krueger J.H., Walker G.C. 1984; groEL and dnaK genes of Escherichia coli are induced by UV irradiation and nalidixic acid in an htpR+-dependent fashion. Proceedings of the National Academy of Sciences of the United States of America 81:1499–1503
    [Google Scholar]
  16. Lindquist S. 1986; The heat shock response. Annual Review of Biochemistry 55:1151–1191
    [Google Scholar]
  17. Massie H.R., Somis H.V., Baird M.B. 1972; The kinetics of degradation of DNA and RNA by H2O2. Biochimica et Biophysica Acta 111:539–548
    [Google Scholar]
  18. Mccord J.M., Day D.D. 1978; Superoxide-dependent production of hydroxyl radical catalyzed by iron-EDTA complex. FEBS Letters 86:139–142
    [Google Scholar]
  19. Neidhardt F.C., VanBogelen R.A., Vaughn V. 1984; The genetics and regulation of the heat-shock proteins. Annual Review of Genetics 18:295–329
    [Google Scholar]
  20. Ipaek H.-H., Walker G. 1987; Escherichia coli dnaK null mutants are inviable at high temperature. Journal of Bacteriology 169:283–290
    [Google Scholar]
  21. Ripley L.S. 1988; Estimation of in vivo miscoding rates. Journal of Bacteriology 202:17–34
    [Google Scholar]
  22. Saito H., Uchida H. 1977; Initiation of the DNA replication of bacteriophage lambda in Escherichia coli K-12. Molecular and General Genetics 164:1–8
    [Google Scholar]
  23. Sakakibara Y. 1988; The dnaK gene of Escherichia coli functions in initiation of chromosome replication. Journal of Bacteriology 170:972–979
    [Google Scholar]
  24. Schlesinger M.J., Ashburner M., Tlssieres A. 1982 Heat Shock from Bacteria to Man Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Setlow B.B. 1987; Theory presentation and background summary. In Modern Biological Theories of Aging p. 177 Warner H.R. Edited by New York: Raven Press;
    [Google Scholar]
  26. Steinberg C.M., Edgar R.S. 1962; A critical test of a current theory of genetic recombination in bacteriophage. Genetics 47:187–208
    [Google Scholar]
  27. Subjeck J.R., sciandra J.J. 1982; Coexpression of thermotolerance and heat shock proteins in mammalian cells. In Heat Shock from Bacteria to Man pp 405–411 Schlesinger M.J., Ashbumer M., Tissieres A. Edited by Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Tilly K., Mckittrick N., Zylicz M., Georgopoulos C. 1983; The dnaK protein modulates the heat-shock response of Escherichia coli. Cell 34:641–646
    [Google Scholar]
  29. VanBogelen R.A., Kelly P.M., Neidhardt F.C. 1987; Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli. Journal of Bacteriology 169:26–32
    [Google Scholar]
  30. Woodcock E., Grigg G.W. 1972; Repair of thermally induced DNA breakage in Escherichia coli. Nature New Biology 237:76–79
    [Google Scholar]
  31. Yamamori T., Yura T. 1982; Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12. Proceedings of the National Academy of Sciences of the United States of America 79:860–864
    [Google Scholar]
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