1887

Microbiology Society logo

Volume 137, Issue 3

Identification of four unique clones encoding 10 kDa proteins from that cause phenotypic complementation of a mutant strain of Free

Abstract

A number of clones have been isolated from two species which complement the PhoA phenotype of mutants under conditions that induce the expression of alkaline phosphatase (APase). These clones were initially thought to carry XPases because the transformed host could hydrolyse a common APase substrate, XP (5-bromo-4-chloro-3-indolyl-phosphate). The sequences of the open reading frames responsible for the phenotypic complementation showed no sequence similarity to APases of , human (bone-liver-kidney, intestinal or placental) or . Therefore, these clones were designated as XPA (for X Phosphatase Activity) clones. Four of the clones encoded small (10 kDa), basic, hydrophobic proteins. Two of these, from 168 and from MC14, shared 62% identity at both the DNA and the predicted amino acid sequence level. The fact that homologues from two strains were cloned indicated that the screen was specific, but not for APase genes. It is clear that phenotypic complementation with cloned DNA from another genus does not ensure the identification of an APase gene. Possible mechanisms for the abnormal phenotypic complementation are discussed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology 1991
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-3-667
1991-03-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/3/mic-137-3-667.html?itemId=/content/journal/micro/10.1099/00221287-137-3-667&mimeType=html&fmt=ahah

References

  1. Berg P. 1981; Cloning and characterization of the Escherichia coligene coding for alkaline phosphatase. Journal of Bacteriology 146:660–667
     [Google Scholar]
  2. Bookstein C., Edwards C. W., Kapp N., Hulett F. M. 1990; The Bacillus subtilis168 alkaline phosphatase III gene : the impact of a phoAmutation on total alkaline phosphatase synthesis. Journal of Bacteriology 172:3730–3737
     [Google Scholar]
  3. Castilho B. A., Olfson P., Casadaban M. J. 1984; Plasmid insertion mutagenesis and lacgene fusion with mini-Mu bacteriophage transposons. Journal of Bacteriology 158:488–495
     [Google Scholar]
  4. Cohen S. N., Chang A. C. Y., Hsu L. 1972; Non-chromosomal antibiotic resistance in bacteria. Genetic transformation of Escherichia coliby R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America 69:2110–2114
     [Google Scholar]
  5. Dideberg O., Charlier P., Dive G., Joris B., Frere J. M., Ghuysen J. M. 1982; Structure of a Zn++-containing D-alanyl-D- alanine-cleaving carboxypeptidase at 2·5 À resolution. Nature London: 299469–470
     [Google Scholar]
  6. Dubnau D., Davidoff-Abelson R., Smith I. 1969; Transformation and transduction in Bacillus subtilis: evidence for separate modes of recombinant formation. Journal of Molecular Biology 45:155–179
     [Google Scholar]
  7. Filloux A., Bally M., Soscia C., Murgier M., Lazdunski A. 1988; Phosphate regulation in Pseudomonas aeruginosa.Cloning of the alkaline phosphatase gene and identification of phoB-and phoR-like genes. Molecular and General Genetics 212:510–513
     [Google Scholar]
  8. Garen A., Levinthal C. 1960; A fine structure genetic and chemical study of the enzyme alkaline phosphatase in E. coli.I. Purification and characterization of alkaline phosphatase. Biochimica et Biophysica Acta 38:470–476
     [Google Scholar]
  9. Ghosh B. K., Wouters J. T. M., Lampen J. O. 1971; Distribution of the sites of alkaline phosphatase(s) activity in vegetative cells of Bacillus subtilis . Journal of Bacteriology 108:928–937
     [Google Scholar]
  10. Ghosh R., Ghosh A., Ghosh B. K. 1977; Properties of the membrane bound alkaline phosphatase from glucose- and lactate- grown cells of Bacillus subtilisSB5. Journal of Biological Chemistry 252:6813–6822
     [Google Scholar]
  11. Glenn A. R. 1975; Alkaline phosphatase mutants of Bacillus subtilis . Australian Journal of Biological Science 28:323–330
     [Google Scholar]
  12. Glenn A. R., Mandelstam J. 1971; Sporulation in Bacillus subtilis168. Comparison of alkaline phosphatase from sporulating and vegetative cells. Biochemical Journal 123:129–138
     [Google Scholar]
  13. Grant W. D. 1974; Sporulation in Bacillus subtilis168. Control of synthesis of alkaline phosphatase. Journal of General Microbiology 82:363–369
     [Google Scholar]
  14. Groisman E. A., Casadaban M. J. 1986; Mini-Mu bacteriophage with plasmid replicons for in vivocloning and lacgene fusion. Journal of Bacteriology 168:357–364
     [Google Scholar]
  15. Hansa J. G., Laporta M., Kuna M. A., Reimschuessel R., Hulett F. M. 1981; A soluble alkaline phosphatase from Bacillus licheniformisMC 14 : histochemical localization, purification and characterization and comparison with the membrane-associated alkaline phosphatase. Biochimica et Biophysica Acta 675:340–401
     [Google Scholar]
  16. Hulett F. M. 1984; Cloning and characterization of the Bacillus licheniformisgene coding for alkaline phosphatase. Journal of Bacteriology 158:978–982
     [Google Scholar]
  17. Hulett F. M., Campbell L. L. 1971a; Purification and properties of an alkaline phosphatase of Bacillus licheniformis . Biochemistry 10:1364–1370
     [Google Scholar]
  18. Hulett F. M., Campbell L. L. 1971b; Molecular weight and subunits of alkaline phosphatase of Bacillus licheniformis . Biochemistry 10:1371–1376
     [Google Scholar]
  19. Hulett F. M., Jensen K. 1988; Critical roles of spoOAand spoOHin vegetative alkaline phosphatase production in Bacillus subtilis . Journal of Bacteriology 170:3765–3768
     [Google Scholar]
  20. Hulett F. M., Schaffel S. D., Campbell L. L. 1976; Subunits of alkaline phosphatase of Bacillus licheniformis: chemical, physicochemical, and dissociation studies. Journal of Bacteriology 128:651–757
     [Google Scholar]
  21. Hulett F. M., Wang P.-Z., Sussman M., Lee J.-W. 1985; Two alkaline phosphatase genes positioned in tandem in B. licheniformisMC 14 which require different RNA polymerase holoenzymes for transcription. Proceedings of the National Academy of Sciences of the United States of America 82:1035–1039
     [Google Scholar]
  22. Hulett F. M., Stuckmann K., Spencer D. B., Sanopoulou T. 1986; Purification and characterization of the secreted alkaline phosphatase of Bacillus licheniformisMC 14: identification of a possible precursor. Journal of General Microbiology 132:2387–2395
     [Google Scholar]
  23. Hulett F. M., Jensen K., Bookstein C., Owen D., Edwards C., Kapp N. 1988; In vitroderived phosphatase mutants of Bacillus . In Genetics and Biotechnology of Bacilli 2325–329 Ganesan A. T., Hoch. J. New York: Academic Press;
     [Google Scholar]
  24. Hulett F. M., Bookstein C., Jensen K. 1990; Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis . Journal of Bacteriology 172:735–740
     [Google Scholar]
  25. Inouye H., Michaelis S., Wright A., Beckwith J. 1981; Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coliand generation of deletion mutants in vitro . Journal of Bacteriology 146:668–675
     [Google Scholar]
  26. Joris B., Beeumen J. V., Casagrande F., Gerday C., Frere J. M., Ghuysen J. M. 1983; The complete amino acid sequence of the Zn++-containing D-alanyl-D-alanine-cleaving carboxypeptidase of Streptomyces albusG. European Journal of Biochemistry 130:53–69
     [Google Scholar]
  27. Kam W., Clauser E., Kim Y. S., Kan Y. W., Rutter W. 1985; Cloning, sequencing, and chromosomal localization of human term placental alkaline phosphatase cDNA. Proceedings of the National Academy of Sciences of the United States of America 82:8715–8719
     [Google Scholar]
  28. Kapp V. K., Edwards C. W., Chesnut R. S., Hulett F. M. 1990; The Bacillus subtilis phoAIVgene: effects of in vitroinactivation on total alkaline phosphatase production. Gene 96:95–100
     [Google Scholar]
  29. Kim E. E., Wyckoff H. W. 1989; Structure and function of alkaline phosphatases: structure of alkaline phosphatases. Clinica Chimica Acta 186:175–188
     [Google Scholar]
  30. Klein P., Kanehisa M., Delisi C. 1985; The detection and classification of membrane-spanning proteins. Biochimica et Biophysica Acta 815:468–476
     [Google Scholar]
  31. Le Hegarat J. C., Anagnostopoulos C. 1973; Purification, subunit structure and properties of two repressible phosphohydro- lases of Bacillus subtilis . European Journal of Biochemistry 39:525–539
     [Google Scholar]
  32. Makino K., Shinagawa H., Amemura M., Kawamoto T., Yamada M., Nakata A. 1989; Signal transduction in the phosphate regulon of Escherichia coliinvolves phosphotransfer between PhoR and PhoB proteins. Journal of Molecular Biology 210:551–559
     [Google Scholar]
  33. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning . A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  34. McCarter L. L., ilverman. M. 1987; Phosphate regulation of gene expression in Vibrio parahaemolyticus . Journal of Bacteriology 169:3441–3449
     [Google Scholar]
  35. Mcnicholas J. M., Hulett F. M. 1977; Electron microscope histochemical localization of alkaline phosphatase(s) in Bacillus licheniformis . Journal of Bacteriology 129:501–515
     [Google Scholar]
  36. Miki T., Minimi Z., Ikeda Y. 1965; The genetics of alkaline phosphatase formation in Bacillus subtilis . Genetics 52:1093–1100
     [Google Scholar]
  37. Neidhardt F. C., Bloch P. L., Smith D. F. 1974; Culture medium for enterobacteria. Journal of Bacteriology 119:736–747
     [Google Scholar]
  38. Piggot P. J., Taylor S. Y. 1977; New types of mutations affecting formation of alkaline phosphatase by Bacillus subtilisin sporulation conditions. Journal of General Microbiology 102:69–80
     [Google Scholar]
  39. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
     [Google Scholar]
  40. Schaeffer P., Millet J., Aubert J. 1965; Catabolite repression of bacterial sporulation. Proceedings of the National Academy of Sciences of the United States of America 54:704–711
     [Google Scholar]
  41. Seki T., Yoshikawa H., Takahashi H., Saito H. 1988; Nucleotide sequence of the Bacillus subtilis phoRgene. Journal of Bacteriology 170:5935–5938
     [Google Scholar]
  42. Shimotsu H., Henner D. J. 1986; Construction of a single-copy integration vector and its use in analysis of regulation of the trpoperon of Bacillus subtilis . Gene 43:85–94
     [Google Scholar]
  43. Shine J., Dalgarno L. 1974; The 3’-terminal sequence of Escherichia coli16S ribosomal RNA : complementarity to nonsense triplets and ribosome binding sites. Proceedings of the National Academy of Sciences of the United States of America 71:1342–1346
     [Google Scholar]
  44. Silhavy T., Berman J., Michael L., Enquist L. W. 1984 Experiments with Gene Fusions205–207 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  45. Spencer D. B., Hulett F. M. 1981; Lactoperoxidase–125I localization of salt-extractable alkaline phosphatase on the cytoplasmic membrane of Bacillus licheniformis . Journal of Bacteriology 145:934–945
     [Google Scholar]
  46. Spencer D. B., Chen C.-P., Hulett F. M. 1981; Effect of cobalt on synthesis and activation of Bacillus licheniformisphosphatase. Journal of Bacteriology 145:926–933
     [Google Scholar]
  47. Spencer D. B., Hansa J., Stuckmann K., Hulett F. M. 1982; A membrane associated alkaline phosphatase from Bacillus licheniformisMC 14 requiring detergent for solubilization : lactoperoxidase125I and molecular weight determination. Journal of Bacteriology 150:826–834
     [Google Scholar]
  48. Stark M. J. R. 1987; Multicopy expression vectors carrying the lacrepressor gene for regulated high-level expression of genes in E. coli . Gene 51:255–267
     [Google Scholar]
  49. Xie W., Whitton B. A., Simon J. W., Jager K., Reed D., Potts M. 1989; Nostoc communeUTEX 584 gene expressing indole phosphate hydrolase activity in E. coli . Journal of Bacteriology 171:708–713
     [Google Scholar]
  50. Yamane K., Maruo B. 1978; Alkaline phosphatase possessing alkaline phosphodiesterase activity and other phosphodiesterases in Bacillus subtilis . Journal of Bacteriology 134:108–114
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-3-667
Loading
Identification of four unique clones encoding 10 kDa proteins from Bacillus that cause phenotypic complementation of a phoA mutant strain of Escherichia coli
Microbiology 137, 667 (1991); https://doi.org/10.1099/00221287-137-3-667
/content/journal/micro/10.1099/00221287-137-3-667
/content/journal/micro/10.1099/00221287-137-3-667
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error