One-step purification of cholesterol oxidase from culture broth of a Pseudomonas sp. using a novel affinity chromatography method

References

1. Allain C. C, Poon L. S., Chan C. S. G., Richmond W., Fu P. C. 1974; Enzymatic determination of total serum cholesterol. Clinical Chemistry 20:470–475
   [Google Scholar]
2. Fukuyama M., Miyake Y. 1979; Purification and some properties of cholesterol oxidase from Schizophyllum commune with covalently bound flavin. Journal of Biochemistry 85:1183–1193
   [Google Scholar]
3. Inoue Y., Taguchi K., Fujii A., Ishimaru K., Nakamura S., Nomi R. 1982; Purification and characterization of extracellular 3β-hydroxy steroid oxidase produced by Streptoverticillium cholesterol icum. Chemical Pharmacology Bulletin 30:951–958
   [Google Scholar]
4. Kamei T., Takiguchi Y., Suzuki H., Matsuzaki M., Nakamura S. 1978; Purification of 3β-hydroxysteroid oxidase of Streptomyces violescens origin by affinity chromatography on cholesterol. Chemical Pharmacology Bulletin 26:2799–2804
   [Google Scholar]
5. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 111680–685
   [Google Scholar]
6. Lee S. Y., Rhee H. I., Tae W. C, Shin J. C., Park B. K. 1989; Purification and characterization of cholesterol oxidase from Pseudomonas sp. and taxonomic study of the stain. Applied Microbiology and Biotechnology 31:542–546
   [Google Scholar]
7. Richmond W. 1973; Preparation and properties of bacterial cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum. Clinical Chemistry 19:1350–1356
   [Google Scholar]
8. Tomioka H., Kagawa M., Nakamura S. 1976; Some enzymatic properties of 3β-hydroxysteroid oxidase produced by Streptomyces violescens. Journal of Biochemistry 79:903–915
   [Google Scholar]
9. Uwajima T., Yagi H., Nakamura S., Terada O. 1973; Isolation and crystallization of extracellular 3/J-hydroxysteroid oxidase of Brevibacterium sterolicum nov. sp. Agricultural and Biological Chemistry 37:2345–2350
   [Google Scholar]