1887

Abstract

Summary: mt-2, harbouring the TOL plasmid PWW0, was grown continuously on benzoate in a phauxostat at a non-limited rate. The gradual decrease in the population carrying the complete TOL plasmid was caused predominantly by a growth-rate advantage of spontaneous mutants carrying a partially deleted plasmid (TOL cells). The growth-rate difference () was quantified both by measuring the increase in the dilution rate (from 0.68 to 0.79 h; = 0.11 h) and by mathematical analysis of the ingrowth of TOL cells ( = 0.12 h). The latter procedure also established that the segregation rate was of the order of magnitude 10 h. Similar values for the growth-rate advantage and the segregation rate were found when both benzoate and succinate were present in non-limiting concentrations. It is suggested that the growth-rate disadvantage of the wild-type strain is caused by inhibitory effects of an intermediate in the degradation of benzoate via the plasmid-encoded -pathway.

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1991-06-01
2024-04-28
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References

  1. Assinder S. J., Williams P. A. 1990; The TOL plasmids: determinants of the catabolism of toluene and the xylenes. Advances in Microbial Physiology 31:1–70
    [Google Scholar]
  2. Bayley S. A., Duggleby C. J., Worsey M. J., Williams P. A., Hardy K. G., Broda P. 1977; Two modes of loss of the TOL function from Pseudomonas putida mt-2. Molecular and General Genetics 154:203–204
    [Google Scholar]
  3. Cooper N. S., Brown M. E., Caulcott C. A. 1987; A mathematical method for analysing plasmid stability in microorganisms. Journal of General Microbiology 133:1871–1880
    [Google Scholar]
  4. Davidson A. M., Dunn A., Day M. J., Randerson P. F. 1990; A nonlinear technique for the analysis of plasmid instability in microorganisms. Journal of General Microbiology 136:59–64
    [Google Scholar]
  5. Duetz W. A., van Andel J. G. 1991; Stability of TOL plasmid pW W0 in Pseudomonas putida mt-2 under non-selective conditions in continuous culture. Journal of General Microbiology 137:1369–1374
    [Google Scholar]
  6. Evans C. G. T., Herbert D., Tempest D. W. 1970; The continuous cultivation of microorganisms. 2. Construction of a chemostat. Methods in Microbiology 2:277–327
    [Google Scholar]
  7. Girvitz S. C., Bacchetti S., Rainbow A. J., Graham F. W. 1980; A rapid and efficient procedure for the purification of DNA from agarose gels. Analytical Biochemistry 106:492–496
    [Google Scholar]
  8. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  9. Kado C. I., Liu S. T. 1981; Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145:1365–1373
    [Google Scholar]
  10. Keil H., Williams P. A. 1985; A new class of TOL plasmid deletion mutants in Pseudomonas putida MT15 and their reversion by tandem gene amplification. Journal of General Microbiology 131:1023–1033
    [Google Scholar]
  11. Keshavarz T., Lilly M. D., Clarke P. H. 1985; Stability of a catabolic plasmid in continuous culture. Journal of General Microbiology 131:1193–1203
    [Google Scholar]
  12. Martin G. A., Hempfling W. P. 1976; A method for the regulation of microbial population density during continuous culture at high growth rates. Archives of Microbiology 107:41–47
    [Google Scholar]
  13. Meulien P., Downing R. G., Broda P. 1981; Excision of the 40 kb segment of the TOL plasmid from Pseudomonas putida mt-2 involves direct repeats. Molecular and General Genetics 184:97–101
    [Google Scholar]
  14. Murray K., Duggleby C. J., Sala-Trepat J. M., Williams P. A. 1972; The metabolism of benzoate and methylbenzoates via the meta-cleavage pathway by Pseudomonas arvilla mt-2. European Journal of Biochemistry 28:301–310
    [Google Scholar]
  15. Nakazawa T., Yokota T. 1973; Benzoate metabolism in Pseudomonas putida(arvilla) mt-2 : demonstration of two benzoate pathways. Journal of Bacteriology 115:262–267
    [Google Scholar]
  16. Noack D., Muller G., Roth M. 1984; Mathematical modelling of genetic segregation kinetics obtained with chemostat cultures of procaryotic microorganisms. Zeitschrift für Allgemeine Mikrobiologie 24:459–465
    [Google Scholar]
  17. Osborne D. J., Pickup R. W., Williams P. A. 1988; The presence of two complete homologous meta pathway operons on TOL plasmid pW W53. Journal of General Microbiology 134:2965–2975
    [Google Scholar]
  18. Stephens G. M., Dalton H. 1987; The effect of lipophilic weak acids on the segregational stability of TOL plasmids in Pseudomonas putida. Journal of General Microbiology 133:1891–1899
    [Google Scholar]
  19. Stephens G. M., Dalton H. 1988; Kinetics of benzoate-induced loss of the TOL plasmid from Pseudomonas putida MT15 during growth in chemostat culture. FEMS Microbiology Letters 55:175–180
    [Google Scholar]
  20. Wheatcroft R., Williams P. A. 1981; Rapid method for the study of both stable and unstable plasmids in Pseudomonas. Journal of General Microbiology 124:433–437
    [Google Scholar]
  21. Wigmore G. J., Bayly R. C., Di Berardino D. 1974; Pseudomonas putida mutants defective in the metabolism of the products oimeta fission of catechol and its methyl analogues. Journal of Bacteriology 120:31–37
    [Google Scholar]
  22. Williams P. A., Murray K. 1974; Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2 : evidence for the existence of a TOL plasmid. Journal of Bacteriology 120:416–423
    [Google Scholar]
  23. Williams P. A., Taylor S. D., Gibb L. E. 1988; Loss of the toluene-xylene catabolic genes of TOL plasmid pW W0 during growth of Pseudomonas putida on benzoate is due to a selective growth advantage of ‘cured’ segregants. Journal of General Microbiology 134:2039–2048
    [Google Scholar]
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