1887

Abstract

Summary: A novel calcium-binding protein has been purified from the dormant spores of T. Purity of this protein was verified by SDS-PAGE and reversed-phase HPLC. Its calcium-binding ability was verified by a competitive calcium-binding assay using Chelex-100 resin and Ca autoradiography. The protein is heat-stable and is retained by hydrophobic matrices (phenyl-Sepharose) in a calcium-dependent manner. SDS-PAGE and amino acid composition indicate the molecular mass of the protein to be 24 kDa.

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/content/journal/micro/10.1099/00221287-137-7-1619
1991-07-01
2024-04-30
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References

  1. Barrantes F. J. 1975; The nicotinic cholinergic receptor: different compositions evidenced by statistical analysis. Biochemical and Biophysical Research Communications 62:407–414
    [Google Scholar]
  2. Bidlingmeyer B. A., Cohen S. A., Tarvin T. L. 1984; Rapid analysis of amino acids using pre-column derivatization. Journal of Chromatography 336:93–104
    [Google Scholar]
  3. Bigelow C. C. 1967; On the average hydrophobicity of proteins and the relation between it and protein structure. Journal of Theoretical Biology 16:187–211
    [Google Scholar]
  4. Burgess W. H., Schleicher M., Van Eldik L. J., Watterson D. M. 1983; Comparative studies of calmodulin. Calmodulin and Cell Function IV209–262 Cheung Y. W. New York: Academic Press;
    [Google Scholar]
  5. Douthit H. A., Preston R. A. 1985; Activation of Bacillus cereus spores with calcium. Fundamental and Applied Aspects of Bacterial Spores297–307 Dring G. J., Ellar D. J., Gould G. W. Orlando, FL: Academic Press;
    [Google Scholar]
  6. Inouye S., Harada W., Zusman D., Inouye M. 1981; Development-specific proteins S of Myxococcus xanthus: purification and characterization. Journal of Bacteriology 148:678–683
    [Google Scholar]
  7. Johnson K. M., Nelson C., Busta F. F. 1982; Germination and heat resistance of Bacillus cereus spores from strains associated with diarrheal and emetic food-borne illness. Journal of Food Science 47:1268–1271
    [Google Scholar]
  8. Johnson W. C., Tipper D. J. 1981; Acid-soluble spore proteins of Bacillus subtilis . Journal of Bacteriology 146:972–982
    [Google Scholar]
  9. Kamat A. S., Lewis N. F., Pradhan D. S. 1985; Mechanism of Ca2+ and dipicolinic acid requirement for l-alanine induced germination of Bacillus cereus BIS-59 spores. Microbios 44:33–44
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  11. Leadlay P. F., Roberts G., Walker J. E. 1984; Isolation of a novel calcium-binding protein from Streptomyces erythreus . FEBS Letters 178:157–160
    [Google Scholar]
  12. Maruyama K., Mikawa T., Ebashi S. 1984; Detection of calcium binding protein by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis. Journal of Biochemistry 95:511–519
    [Google Scholar]
  13. Montelaro R. C. 1987; Protein antigen purification by preparative protein blotting. Electrophoresis 8:432–438
    [Google Scholar]
  14. Nakatani T., Tani K., Imagawa M., Nishihara N., Kondo M. 1985; Germination of coat-lacking spores of Bacillus megaterium . Biochemical and Biophysical Research Communications 128:728–732
    [Google Scholar]
  15. Randolph A., Strothkamp K. G. 1988; Instability of purified 9- kDa porcine intestinal calcium-binding protein in the absence of calcium. Archives of Biochemistry and Biophysics 267:46–53
    [Google Scholar]
  16. Rode L. J., Foster J. W. 1966; Quantitative aspects of exchangeable calcium in spores of Bacillus megaterium . Journal of Bacteriology 91:1589–1593
    [Google Scholar]
  17. Rowley D. B., Levinson H. S. 1967; Changes in spores of Bacillus megaterium treated with thioglycolate at a low pH and restoration of germinability and resistance by cations. Journal of Bacteriology 93:1017–1022
    [Google Scholar]
  18. Sacks L. E. 1972; Influence of intra- and extracellular cations on the germination of bacterial spores. Spores V437–443 Halvorson H. O., Hanson R., >Campbell L. L. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Setlow P. 1985; Protein degradation during bacterial spore germination. Fundamental and Applied Aspects of Bacterial Spores285–296 Ellar D. J. London: Academic Press;
    [Google Scholar]
  20. Tokuda M., Khanna N. C., Waisman D. M. 1987; Purification of novel calcium-binding proteins from bovine brain. Methods in Enzymology 139:68–79
    [Google Scholar]
  21. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 764350–4354
    [Google Scholar]
  22. Watterson D. M., Sharief F. S., Vanamam T. C. 1980; The complete amino acid sequence of the Ca2+-dependent modulator protein of bovine brain. Journal of Biological Chemistry 255:962–975
    [Google Scholar]
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