1887

Abstract

Summary: was grown in a chemostat under conditions of glutamate limitation. The effects of growth rate on production of the antibiotic granaticin, extracellular protein and protease activity as components of secondary metabolism were studied at 37, 45 and 50 °C. The amount of each secondary metabolite synthesized was highly dependent on growth rate and temperature. Granaticin yields were highest at growth rates of 0·1 to 0·15 h at 37 °C, 0·175 h at 45 °C and 0·045 h at 50 °C. Protease activity of culture supernatants responded to low nutrient concentration and/or low growth rate. Measurements of extracellular protein revealed complex changes in amount which were dependent on growth rate and temperature. At 45 °C and a growth rate of 0·15 h, biomass yield was highest between pH 5·5 to 6·5 whereas granaticin synthesis was low at pH 5·5 and rose to highest values at between pH 6·5 and 7·5.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-7-1715
1991-07-01
2024-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/7/mic-137-7-1715.html?itemId=/content/journal/micro/10.1099/00221287-137-7-1715&mimeType=html&fmt=ahah

References

  1. Amner W., McCarthy A. J., Edwards C. 1988; Quantitative assessment of factors affecting the recovery of indigenous and released thermophilic bacteria from compost.. Applied and Environmental Microbiology 54:3107–3112
    [Google Scholar]
  2. Cimburkova E., Zima J., Novak J., Vanek Z. 1988; Nitrogen regulation of avermectins biosynthesis in Streptomyces avermitilis in a defined medium.. Journal of Basic Microbiology 28:491–499
    [Google Scholar]
  3. Dekleva M. L., Titus J. A., Strohl W. R. 1985; Nutrient effects on anthracycline production by Streptomyces peuticus in a defined medium.. Canadian Journal of Microbiology 31:287–294
    [Google Scholar]
  4. Demain A. L., Kennel Y. M., Aharonowitz Y. 1979; Carbon catabolite regulation of secondary metabolism.. Symposia of the Society for General Microbiology 29:163–185
    [Google Scholar]
  5. Demain A. L. 1982; A new era of exploitation of microbial metabolites.. Biochemical Society Symposia 48:117–132
    [Google Scholar]
  6. Edwards C., Ball A. S. 1987; Respiratory chain composition and activity in some thermotolerant streptomycetes.. FEMS Microbiology Letters 40:61–66
    [Google Scholar]
  7. Gibb G. D., Ordaz D. E, Strohl W. R. 1989; Over production of extracellular protease activity by Streptomyces C5-A13 in fed-batch fermentation.. Applied Microbiology and Biotechnology 31:119–124
    [Google Scholar]
  8. Goodfellow M., Lacey J., Todd C. 1987; Numerical classification of thermophilic Streptomyces . Journal of General Microbiology 133:3135–3149
    [Google Scholar]
  9. Hu W. S., Brana A. F., Demain A. L. 1984; Carbon source regulation of cephem antibiotic production by resting cells of Streptomyces clavuligerus and its reversal by protein inhibitors.. Enzyme and Microbial Technology 6:155–160
    [Google Scholar]
  10. James P. D. A., Edwards C. 1988; The effects of cultural conditions on growth and secondary metabolism in Streptomyces thermoviolaceus . FEMS Microbiology Letters 52:1–6
    [Google Scholar]
  11. James P. D. A., Edwards C. 1989; The effects of temperature on growth and production of the antibiotic granaticin by a thermotolerant streptomycete.. Journal of General Microbiology 135:1997–2003
    [Google Scholar]
  12. James P. D. A., Edwards C. 1991; Protease activity in antibiotic- producing Streptomyces thermoviolaceus . Current Microbiology (in the Press)
    [Google Scholar]
  13. Jones C. W., Morgan H. W., Daniel R. M. 1988; Aspects of protease production by a Thermus strain Ok6 and other New Zealand isolates.. Journal of General Microbiology 134:191–198
    [Google Scholar]
  14. Matsue M. J., Drent W. J., Gottschal J. S. 1987; Fermentation of glutamate by Selenomonas acidamiophila sp. nov. Archives of Microbiology 147:152–157
    [Google Scholar]
  15. Payne G. F., Wang H. Y. 1989; The effect of feedback regulation and in situ product removal on the conversion of sugar to cycloheximide.. Archives of Microbiology 151:331–335
    [Google Scholar]
  16. Renko M., Vitale L., Kokalj M., Pokorny M. 1989; Streptomyces rimosus extracellular proteases.. Applied Microbiology and Biotechnology 31:38–44
    [Google Scholar]
  17. Rhodes P. M. 1984; The production of Oxytetracycline in chemostat culture.. Biotechnology Bioengineering 26:382–385
    [Google Scholar]
  18. Shapiro S., Vining L. C. 1983; Nitrogen metabolism and chloramphenicol production in Streptomyces venezuelae . Canadian Journal of Microbiology 29:1706–1714
    [Google Scholar]
  19. Tanaka Y., Taki A., Masuma R., Omura S. 1985; Mechanism of nitrogen regulation of protylonolide biosynthesis in Streptomyces fradiae . Journal of Antibiotics 39:813–821
    [Google Scholar]
  20. Trilli A., Crossley M. V., Kontaku M. 1987; Relation between growth rate and erythromycin production in Streptomyces erythraeus . Biotechnology Letters 9:765–770
    [Google Scholar]
  21. Vu-trong K., Gray P. P. 1987; Influence of ammonium on the biosynthesis of the macrolide antibiotic tylosin.. Enzyme and Microbial Technology 9:590–593
    [Google Scholar]
  22. Zhang J., Wolfle S., Demain A. L. 1989; Ammonium ions repress δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase in Streptomyces clavuligerus NRRL 3585.. Canadian Journal of Microbiology 35:399–402
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-7-1715
Loading
/content/journal/micro/10.1099/00221287-137-7-1715
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error