1887

Abstract

Pyrimidine base catabolism in ATCC 17588 was investigated and was found to occur by means of the reductive pathway. Pyrimidine bases and their respective reductive pathway catabolic products could serve as nitrogen sources for growth of . Activities of the three enzymes associated with the reductive pathway of pyrimidine catabolism were detected in cells of . The initial enzyme of the reductive pathway, dihydropyrimidine dehydrogenase, utilized NADH as its nicotinamide cofactor. Cells grown on pyrimidine bases as nitrogen sources contained elevated dehydrogenase activity relative to those grown on ammonium sulphate as nitrogen source. Activities of the second and third reductive pathway enzymes, dihydropyrimidinase and -carbamoyl-β-alanine amidohydrolase, respectively, were also affected by growth conditions. If pyrimidine or dihydropyrimidine bases served as nitrogen sources, increases in the levels of these enzymes were observed compared to their activities determined when the nitrogen source was ammonium sulphate.

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/content/journal/micro/10.1099/00221287-138-11-2459
1992-11-01
2024-05-04
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References

  1. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  2. Campbell L. L. Jr 1957; Reductive degradation of pyrimidines. II. Mechanism of uracil degradation by Clostridium uracilicum. Journal of Bacteriology 73:225–229
    [Google Scholar]
  3. Chevalier P., Roy D., Morin A. 1989; Hydantoinase activity of immobilized non-growing Pseudomonas putida cells. Applied Microbiology and Biotechnology 30:482–486
    [Google Scholar]
  4. Hayaishi O., Kornberg A. 1952; Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes. Journal of Biological Chemistry 197:717–732
    [Google Scholar]
  5. Hunninghake D., Grisolia S. 1965; Uracil and thymine reductases. Methods in Enzymology 12A:50–59
    [Google Scholar]
  6. Kaplan N. O. 1955; Pyridine nucleotide transhydrogenase. Methods in Enzymology 2:681–687
    [Google Scholar]
  7. Kim S., West T. P. 1991; Pyrimidine catabolism in Pseudomonas aeruginosa. FEMS Microbiology Letters 77:175–180
    [Google Scholar]
  8. Kramer J., Kaltwasser H. 1969; Verwertung von pyrimidinderivaten <lurch Hydrogenomonas facilis. I. Intermediarprodukte und enzyme des cytosinabbaues. Archiv for Mikrobiologie 68:227–235
    [Google Scholar]
  9. Lara F. J. S. 1952; On the decomposition of pyrimidines by bacteria. I. Studies by means of the technique of simultaneous adaption. Journal of Bacteriology 64:271–277
    [Google Scholar]
  10. Morin A., Hummel W., Kula M.-R. 1986; Production of hydantoinase from Pseudomonas fluorescens strain DSM 84. Applied Microbiology and Biotechnology 25:91–96
    [Google Scholar]
  11. Patel B. N., West T. P. 1987; Oxidative catabolism of uracil by Enterobacter aerogenes. FEMS Microbiology Letters 40:33–36
    [Google Scholar]
  12. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology 43:159–271
    [Google Scholar]
  13. Sun W. 1983; Screening of strains producing dihydropyrimidinase and fermentation conditions. Weishengwu Xuebao 23:133–142
    [Google Scholar]
  14. Takahashi S., Kn Y., Kumagai H., Yamada H. 1978; Purification, crystallization and properties of hydantoinase from Pseudomonas striata. Journal of Fermentation Technology 56:492–498
    [Google Scholar]
  15. Tamaki N., Mizutani N. 1987; Purification and properties of P-ureidopropionase from the rat liver. European Journal of Biochemistry 169:21–26
    [Google Scholar]
  16. Vogels G. D., Van Der Drift C. 1976; Degradation of purines and pyrimidines by microorganisms. Bacteriological Reviews 40:403–468
    [Google Scholar]
  17. West T. P. 1989; Isolation and characterization of thymidylate synthetase mutants of Xanthomomas maltophilia. Archives of Microbiology 151:220–222
    [Google Scholar]
  18. West T. P. 1991a; Pyrimidine base and ribonucleoside utilization of the Pseudomonas alcaligenes group. Antonie van Leeuwenhoek 59:263–268
    [Google Scholar]
  19. West T. P. 1991b; Isolation and characterization of a dihydropyrimidine dehydrogenase mutant of Pseudomonas chlororaphis. Archives of Microbiology 156:513–516
    [Google Scholar]
  20. West T. P., Shanley M. S., O’Donovan G. A. 1982; Improved colorimetric procedure for quantitating N-carbamoyl-P-alanine with minimum dihydrouracil interference. Analytical Biochemistry 122:345–347
    [Google Scholar]
  21. West T. P., Traut T. W., Shanley M. S., O’Donovan G. A. 1985; A Salmonella typhimurium strain defective in uracil catabolism and P-alanine synthesis. Journal of General Microbiology 131:1083–1090
    [Google Scholar]
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