1887

Abstract

Summary: 569B was found to be highly sensitive to a wide range of chemicals, particularly hydrophobic compounds and neutral and anionic detergents. The phospholipid profile of the outer membrane was similar to that reported for other Gram-negative bacteria. The lipopolysaccharide (LPS) contained -antigenic sugars and exhibited heterogeneity. In addition, the LPS moiety was characterized by a relatively low negative charge. Analysis by topological probes revealed the presence of a significant amount of exposed phospholipids in the outer membrane. The reduced negative charge of LPS molecules and the exposed phospholipids present in the outer membrane could be important in the increased permeation of exogenous compounds in

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-4-755
1992-04-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/4/mic-138-4-755.html?itemId=/content/journal/micro/10.1099/00221287-138-4-755&mimeType=html&fmt=ahah

References

  1. CANNON J. G. , SPARLING P. F. . 1984; The genetics of Gonococcus. Annual Review of Microbiology 38:111–133
    [Google Scholar]
  2. CHAKRABARTI D. , CHATTERJEE Α. Ν. . 1984; Studies on heterogeneous lipopolysaccharide fractions of Vibrio cholerae 569B. Journal of General Microbiology 130:2023–2026
    [Google Scholar]
  3. CHATTERJEE S. N. , DAS J. . 1967; Electron microscopic observations on the excretion of cell wall material by Vibrio cholerae. Journal of General Microbiology 49:1–11
    [Google Scholar]
  4. FILIP C. , FLETCHER G. , WULFF J. L. , EARHART C. F. . 1973; Solubilization of cytoplasmic membrane of E. coli by the ionic detergent sodium lauryl sarcosinate. Journal of Bacteriology 115:717–722
    [Google Scholar]
  5. GHUYSEN J. M. , STROMINGER J. L. . 1963; Structure of the cell wall of Staphylococcus aureus, strain Copenhagen. 1. Preparation of fragments by enzymatic hydrolysis. Biochemistry 2:1110–1119
    [Google Scholar]
  6. GUSTAFSSON B. , HOLME T. . 1985; Immunological characterization of Vibrio cholerae 0:1 lipopolysaccharide, O-side chain and core with monoclonal antibodies. Infection and Immunity 49:275–280
    [Google Scholar]
  7. HITCHCOCK P. J. , LEIVE L. , MAKELA P. H. , RIETSCHEL E. TH. , STRITMATTER W. , MORRISON D. C. . 1986; Lipopolysaccharide nomenclature - past, present and future. Journal of Bacteriology 166:699–705
    [Google Scholar]
  8. INUKAI M. , TAKEUCHI M. , SHIMIZU K. , ARAI M. . 1978; Mechanism of action of globomycin. Journal of Antibiotics 31:1203–1205
    [Google Scholar]
  9. KAMIO Y. , NIKAIDO H. . 1976; Accessibility of phospholipid head groups to phospholipase C and cyanogen bromide activated dextran in the external medium. Biochemistry 15:2561–2570
    [Google Scholar]
  10. KINOSHITA T. , IINUMA F. , TSUJI A. . 1974; Fluorescent labelling of proteins and a plasma membrane using cycloheptaamylose-dansyl chloride complex. Analytical Biochemistry 61:632–637
    [Google Scholar]
  11. KOPLOW J. , GOLDFINE H. . 1974; Alterations in the outer membrane of the cell envelope of heptose-deficient mutants of Escherichia coli. Journal of Bacteriology 117:525–542
    [Google Scholar]
  12. LOHIA Α. , CHATTERJEE A. N. , DAS J. . 1984; Lysis of Vibrio cholerae cells: direct isolation of the outer membrane from whole cells by treatment with urea. Journal of General Microbiology 130:2027–2033
    [Google Scholar]
  13. LOHIA Α. , MAJUMDAR S. , CHATTERJEE A. N. , DAS J. . 1985; Effect of changes in the osmolarity of the growth medium on Vibrio cholerae cells. Journal of Bacteriology 163:1158–1166
    [Google Scholar]
  14. LUGTENBERG B. , VAN ALPHEN L. . 1983; Molecular architecture of the outer membrane of Escherichia coli and other Gram-negative bacteria. Biochimica et Biophysica Acta 737:51–115
    [Google Scholar]
  15. LYSKO P. A. , MORSE S. A. . 1981; Neisseria gonorrhoeae cell envelope: permeability to hydrophobic molecules. Journal of Bacteriology 145:946–952
    [Google Scholar]
  16. MARKWELL Μ. Α. K. , HAAS S. M. , BIEBER L. L. , TOLBERT Ν. E. . 1978; A modification of the Lowry procedure to simplify protein determination in the membrane and lipoprotein samples. Analytical Biochemistry 87:206–210
    [Google Scholar]
  17. NEILL R. J. , IVINS B. E. , HOLMES R. K. . 1983; Synthesis and secretion of the plasmid-coded heat-labile enterotoxin of Escherichia coli in Vibrio cholerae. Science 221:289–291
    [Google Scholar]
  18. NEU C. H. , HEPPEL L. A. . 1965; The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. Journal of Biological Chemistry 240:3685–3692
    [Google Scholar]
  19. NIKAIDO H. , VAARA M. . 1985; Molecular basis of bacterial outer membrane permeability. Microbiological Reviews 49:1–32
    [Google Scholar]
  20. OSBORN M. J. , GANDER J. E. , PARISI E. , CARSON J. . 1972; Mechanism of assembly of the outer membrane of Salmonella typhimurium. Journal of Biological Chemistry 247:3962–3972
    [Google Scholar]
  21. PAUL S. , SEN A. K. , BANERJEE N. , CHATTERJEE A. N. , DAS J. . 1990; Lipid A mutants of Vibrio cholerae: isolation and partial characterization. Biochemical and Biophysical Research Communications 169:116–122
    [Google Scholar]
  22. RAY P. , SENGUPTA A. , DAS J. . 1984; Phosphate repression of phage protein synthesis during infection by choleraphage ϕ149. Virology 136:110–124
    [Google Scholar]
  23. ROY N. K. , GHOSH R. K. , DAS J. . 1982; Monomeric alkaline phosphatase of Vibrio cholerae. Journal of Bacteriology 150:1033–1039
    [Google Scholar]
  24. SCHINDLER P. R. G. , TEUBER M. . 1978; Ultrastructural study of Salmonella typhimurium treated with membrane active agents: specific reaction of dansyl chloride with cell envelope components. Journal of Bacteriology 135:198–206
    [Google Scholar]
  25. SCHLECHT S. , WESTPHAL O. . 1970; Untersuchungen zur typisierung von Salmonella-R-formen. Zentralblatt für Bakteriologie Parasitenkunde Infektionskrandheiten und Hygiene Abteilung 1 Originale 213:356–381
    [Google Scholar]
  26. SCHMIDT-ULLRICH R. , KNUFERMANN H. , WALLACH D. F. H. . 1973; The reaction of 1-dimethylaminonaphthalene-5-sulfonyl chloride with erythrocyte membranes. Biochimica et Biophysica Acta 307:353–365
    [Google Scholar]
  27. SMIT J. , KAMIO Y. , NIKAIDO H. . 1975; Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants. Journal of Bacteriology 124:942–958
    [Google Scholar]
  28. STAN-LOTTER H. , GUPTA M. , SANDERSON K. E. . 1979; The influence of cations on the permeability of the outer membrane of Salmonella typhimurium and other Gram-negative bacteria. Canadian Journal of Microbiology 25:475–485
    [Google Scholar]
  29. SUKUPOLVI S. , VAARA M. . 1989; Salmonella typhimurium and Escherichia coli mutants with increased outer membrane permeability to hydrophobic compounds. Biochimica et Biophysica Acta 988:377–387
    [Google Scholar]
  30. VAARA M. , VAARA T. , JENSON M. , HELANDER L. , NURIMEN M. , RIETSCHEL Ε. TH. , MAKELA P. M. . 1981; Characterization of the lipopolysaccharide from the polymyxin-resistant pmrA mutants of Salmonella typhimurium. FEBS Letters 129:145–149
    [Google Scholar]
  31. VAARA M. , NIKAIDO H. . 1984 In Handbook of Endotoxins 11–45 E. Th. Rietschel . Amsterdam: Elsevier;
    [Google Scholar]
  32. WOLF-WATZ H. , ELMROS T. , NORMARK S. , BLOOM G. D. . 1975; Cell envelope of Neisseria gonorrhoeae: composition of penicillin-sensitive and resistant strains. Infection and Immunity 11:1332–1341
    [Google Scholar]
  33. WRIGHT Β. E. , REBERS P. A. . 1972; Procedure for determining heptose and hexose in lipopolysaccharide: modification of the cysteine-sulphuric acid method. Analytical Biochemistry 49:307–319
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-4-755
Loading
/content/journal/micro/10.1099/00221287-138-4-755
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error