1887

Abstract

SUMMARY: It has been long known that the migrating slugs of the cellular slime moulds are highly sensitive to their environment and orient towards light and in temperature and chemical gradients. There is considerable evidence from past work that these orientations are governed by NH which affects the rate of movement of cells within the slug with such precision that orientation to the external stimuli is achieved. In order to test this hypothesis further, various ways to alter the internal NH concentration were devised. Substances that either increased or decreased proteolysis were applied to one side of the tip of a slug, thereby affecting its orientation. Some of the treatments strongly support the role of internally produced NH in orientation, and all the treatments produce results that are consistent with the hypothesis.

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1993-10-01
2024-04-26
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References

  1. Bonner J.T., Dodd M.R. 1962; Evidence for gas induced orientation in the cellular slime molds.. Developmental Biology 5:344–361
    [Google Scholar]
  2. Bonner J.T., Suthers H.B., Odell G.M. 1986; Ammonia orients cell masses and speeds up aggregating cells of slime molds.. Nature, London 323:630–632
    [Google Scholar]
  3. Bonner J.T., Chiang A., Lee L., Suthers H.B. 1988; The possible role of ammonia in phototaxis of migrating slugs of Dictyostelium discoideum.. Proceedings of the National Academy of Sciences of the United States of America 853885–3887
    [Google Scholar]
  4. Bonner J. T., Har D., Suthers H. B. 1989; Ammonia and thermotaxis - further evidence for a central role of ammonia in the directed cell mass movements of Dictyostelium discoideum.. Proceedings of the National Academy of Sciences of the United States of America 862733–2736
    [Google Scholar]
  5. Feit L.N., Sollitto R.B. 1987; Ammonia is the gas used for the spacing of fruiting bodies in the cellular slime mold.. Dictyostelium discoideum. Differentiation 33:193–196
    [Google Scholar]
  6. Kalant H. 1971; Absorption, diffusion, distribution, and elimination of ethanol.. In The Biology of Alcoholism,vol. 1,Biochemistry pp. 1–62 Edited by Kessin B., Begleiter H. New York: Plenum Press;
    [Google Scholar]
  7. Kay R.R., Gadian D.G., Williams S.R. 1986; Intracellular pH in Dictyostelium: a 31P nuclear magnetic resonance study of its regulation and possible role in controlling cell differentiation.. Journal of Cell Science 83:165–179
    [Google Scholar]
  8. Kosugi T., Inouye K. 1989; Negative chemotaxis to ammonia and other weak bases by migrating slugs of the cellular slime moulds.. Journal of General Microbiology 135:1589–1598
    [Google Scholar]
  9. North M.J. 1982; A study of the proteinase activity released by Dictyostelium discoideum during starvation.. Journal of General Microbiology 128:1653–1660
    [Google Scholar]
  10. North M.J., Harwood J.M. 1979; Multiple acid proteinases in the cellular slime mould Dictyostelium discoideum.. Biochimica et Biophysica Acta 566:222–233
    [Google Scholar]
  11. Orton J.M., Sardesai V.M. 1971; Protein, nucleotide, and porphyrin metabolism.. In The Biology of Alcoholism,vol. 1,Biochemistry pp. 229–261 Edited by Kessin B., Begleiter H. New York: Plenum Press;
    [Google Scholar]
  12. Raekallio J. 1970 Enzyme Histochemistry of Wound Healing pp. 90–94 Portland: Gustav Fischer Verlag;
    [Google Scholar]
  13. Schindler J., Sussman M. 1977; Ammonia determines the choice of morphogenetic pathways in Dictyostelium discoideum.. Journal of Molecular Biology 116:161–169
    [Google Scholar]
  14. Smith E., Williams K.L. 1979; Preparation of slime sheath from Dictyostelium discoideum.. FEMS Microbiology Letters 6:119–122
    [Google Scholar]
  15. Takeuchi I., Yabuno K. 1970; Disaggregation of slime mold pseudoplasmodia using EDTA and various proteolytic enzymes.. Experimental Cell Research 61:183–190
    [Google Scholar]
  16. Van DUIJN B., Inouye K. 1991; Regulation of movement speed by intracellular pH during Dictyostelium discoideum chemotaxis.. Proceedings of the National Academy of Sciences of the United States of America 884951–4955
    [Google Scholar]
  17. Wallgren H. 1971; Effect of ethanol on intracellular respiration and cerebral function.. In The Biology of Alcoholism, vol. 1, Biochemistry, pp. 103–125 Edited by Kessin B., Begleiter H. New York: Plenum Press;
    [Google Scholar]
  18. Whitaker B.D., Poff K.L. 1980; Thermal adaptation of thermosensing and negative thermotaxis in Dictyostelium.. Experimental Cell Research 128:87–93
    [Google Scholar]
  19. Whitfield F.E. 1964; The use of proteolytic and other enzymes in the separation of slime mould grex.. Experimental Cell Research 36:62–72
    [Google Scholar]
  20. Zackson S.J., Steinberg M.S. 1989; Axolotl pronephric duct cell migration is sensitive to phosphatidylinositol-specific phospholipase C.. Development 105:1–7
    [Google Scholar]
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