1887

Abstract

SUMMARY: The formation of invertase in was studied by the continuous culture technique. Invertase formation was markedly inhibited by glucose in the culture medium at concentrations greater than 0·001% (w/v), by galactose and lactose at all concentrations tested, and by several other carbon sources. The mean generation time of the organisms and the concentration of ammonia and growth factors had no significant effect on invertase formation. The pH value of the growth medium modified the amount of enzyme formed. The invertase activity, together with non-enzymic hydrolysis of sucrose by the acidic culture medium, was sufficient to account for the disappearance of the observed amount of sucrose from media containing sucrose.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-14-1-109
1956-02-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/14/1/mic-14-1-109.html?itemId=/content/journal/micro/10.1099/00221287-14-1-109&mimeType=html&fmt=ahah

References

  1. Bigger L.C. 1952; Amino-acid metabolism in Saccharomyces fragilis. . Ph.D.Thesis Cambridge.:
    [Google Scholar]
  2. Booth V.H., Green D.E. 1938; A wet crushing mill for micro-organisms. Biochem.J. 32:855
    [Google Scholar]
  3. Davies R. 1953; Enzyme formation in Saccharomyces fragilis. 1. Invertase and raffinase. Biochem.J. 55:484
    [Google Scholar]
  4. Davies R., Falkiner E.A., Wilkinson J.F., Peel J.L. 1951; Ester formation in yeasts. 1. Ethyl acetate formation by Hansenula species. Biochem.J. 49:58
    [Google Scholar]
  5. Folin O., Wu H. 1919; A system of blood analysis. J. Biol. Chem. 38:81
    [Google Scholar]
  6. Gale E.F. 1938; Factors influencing bacterial deamination. 3. Aspartase II. Its occurrence in and extraction from Bacterium coli and its activation by adenosine and related compounds. Biochem.J. 32:1583
    [Google Scholar]
  7. Gale E.F. 1940; Production of amines by bacteria. 1. Decarboxylation of amino- acids by strains of Bacterium coli. Biochem. J. 34:392
    [Google Scholar]
  8. Gale E.F. 1943; Factors influencing the enzymic activities of bacteria. Bact. Rex 7:139
    [Google Scholar]
  9. Gale E.F., Epps H.M.R. 1942; Effect of the pH of the medium during growth on the enzymic activities of bacteria (Escherichia coli and Micrococcus lyso- deikticus) and the biological significance of the changes produced. Biochem. J. 36:600
    [Google Scholar]
  10. Gale E.F., Stephenson M. 1938; Factors influencing bacterial deamination. 2. Factors influencing the activity of DL-serine deaminase in Bacterium coli. Biochem.J. 32:392
    [Google Scholar]
  11. Hestrin S., Lindegren C.C. 1952; Carbohydrases in Saccharomyces haploid stocks of defined genotype. II. Gene controlled induction of glucosidases by a-glucosides. Arch. Biochem. Biophys. 38:317
    [Google Scholar]
  12. Markham R. 1942; A stream distillation apparatus suitable for micro-Kjeldahl analysis. Biochem.J. 36:790
    [Google Scholar]
  13. Monod J. 1950; La technique de culture continue. Theorie et applications. Ann. Inst.Pasteur 79:390
    [Google Scholar]
  14. Novick A., Szilard L. 1950a; Description of the chemostat. Science 112:715
    [Google Scholar]
  15. Novick A., Szilard L. 1950b; Experiments with the chemostat on spontaneous mutations of bacteria. Proc. nat. Acad. Sci., Wash. 36:708
    [Google Scholar]
  16. Reithel F.J. 1951; Fermentation by yeasts in the presence of azide. Northw. Sci. 25:38
    [Google Scholar]
  17. Wooldridge W.R., Knox R., Glass V. 1936; Variability in the activity of bacterial enzymes. I. The effect of the age of the culture. Biochem.J. 30:926
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-14-1-109
Loading
/content/journal/micro/10.1099/00221287-14-1-109
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error