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Volume 143, Issue 11

Characterization of a gene encoding dihydrolipoamide dehydrogenase of the cyanobacterium sp. strain PCC 6803 Free

Abstract

The authors previously reported the isolation and partial characterization of a periplasmically located dihydrolipoamide dehydrogenase (LPD) from the cyanobacterium sp. strain PCC 6803. In the present work the gene ( database accession number Z48564) encoding the apoprotein of this LPD in PCC 6803 has been identified, sequenced and analysed. The gene codes for a protein starting with methionine, which is post-translationally removed. The mature protein contains an N-terminal serine and consists of 473 amino acids with a deduced molecular mass of 51421 Da (including one FAD). The LPD is an acidic protein with a calculated isoelectric point of 5.17. Comparison of the amino acid sequence of the LPD with protein sequences in the databases revealed that the enzyme shares identities of 31-35% with all 18 LPDs so far sequenced and published. As a first step in determining the role of this cyanobacterial LPD, attempts were made to generate an LPD-free mutant by insertionally inactivating the gene with a kanamycin-resistance cassette. However, the selected transformants appeared to be heteroallelic, containing both the intact gene and the gene inactivated by the drug-resistance cassette. The heteroallelic mutant studied, which had about 50% of the wild-type LPD activity, caused acidification of the growth medium. Growth over a prolonged time was only possible after an increased buffering of the medium. Since it is reported in the literature that inactivation of the pyruvate dehydrogenase complex (PDC) leads to acidosis, a function of the LPD in a cytoplasmic-membrane-associated PDC is conceivable.

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Keyword(s): cyanobacteria , dihydrolipoamide dehydrogenase , IpdA gene and Synechocystis
Society for General Microbiology 1997
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1997-11-01
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Characterization of a gene encoding dihydrolipoamide dehydrogenase of the cyanobacterium Synechocystis sp. strain PCC 6803
Microbiology 143, 3543 (1997); https://doi.org/10.1099/00221287-143-11-3543
/content/journal/micro/10.1099/00221287-143-11-3543
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