f A neutral trehalase gene from Candida albicans: molecular cloning, characterization and disruption
- Authors: Raimund Eck, Clemens Bergmann, Karl Ziegelbauer, Wolfgang Schönfeld, Waldemer Künkel
- Author for correspondence: Raimund Eck. Tel: + 49 3641 656852. Fax: +49 3641 656652. e-mail: firstname.lastname@example.org
- Microbiology, December 1997 143: 3747-3756, doi: 10.1099/00221287-143-12-3747
- Subject: Genetics And Molecular Biology
- Published Online:
A neutral trehalase gene, NTC1, from the human pathogenic yeast Candida albicans was isolated and characterized. An ORF of 2724 bp was identified encoding a predicted protein of 907 amino acids and a molecular mass of 104 kDa. A single transcript of approximately 3.2 kb was detected by Northern blot analysis. Comparison of the deduced amino acid sequence of the C. albicans NTC1 gene product with that of the Saccharomyces cerevisiae NTH1 gene product revealed 57% identity. The NTC1 gene was localized on chromosome 1 or R. A null mutant (Δntc1/Δntc1) was constructed by sequential gene disruption. Extracts from mutants homozygous for neutral trehalase deletion had only marginal neutral trehalase activity. Extracts from heterozygous mutants showed intermediate activities between extracts from the wild-type strain and from the homozygous mutants. The null mutant showed no significant differences in pathogenicity as compared to the wild-type strain in a mouse model of systemic candidiasis. This result indicates that the neutral trehalase of C. albicans is not a potential target for antifungal drugs.
© Society For General Microbiology 1997 | Published by the Microbiology Society
Article metrics loading...
Full text loading...
Author and Article Information
/content/journal/micro/10.1099/00221287-143-12-3747dcterms_title,dcterms_subject,pub_serialTitlepub_serialIdent:journal/micro AND -contentType:BlogPost64
/content/journal/micro/10.1099/00221287-143-12-3747dcterms_title,dcterms_subject-pub_serialIdent:journal/micro AND -contentType:BlogPost64
Figure data loading....