f A DNA-binding protein from Candida albicans that binds to the RPG box of Saccharomyces cerevisiae and the telomeric repeat sequence of C. albicans
- Authors: Nobuya Ishii, Mutumi Yamamoto, Hans-Werner Lahm, Shinnji Lizumi, Fumie Yoshihara, Hironobu Nakayama, Mikio Arisawa, Yuhko Aoki3
- 3Author for correspondence: Yuhko Aoki. Tel: +81 467 47 6773. Fax: +81 467 46 5320. e-mail: email@example.com
- Microbiology, February 1997 143: 417-427, doi: 10.1099/00221287-143-2-417
- Subject: Candida Albicans
- Published Online:
Electromobility shift assays with a DNA probe containing the Saccharomyces cerevisiae ENO1 RPG box identified a specific DNA-binding protein in total protein extracts of Candida albicans. The protein, named Rbf1p (RPG-box-binding protein 1), bound to other S. cerevisiae RPG boxes, although the nucleotide recognition profile was not completely the same as that of S. cerevisiae Rap1p (repressor-activator protein 1), an RPG-box-binding protein. The repetitive sequence of the C. albicans chromosomal telomere also competed with RPG-box binding to Rbf1p. For further analysis, we purified Rbf1p 57600-fold from C. albicans total protein extracts, raised mAbs against the purified protein and immunologically cloned the gene, whose ORF specified a protein of 527 aa. The bacterially expressed protein showed RPG-box-binding activity with the same profile as that of the purified one. The Rbf1p, containing two glutamine-rich regions that are found in many transcription factors, showed transcriptional activation capability in S. cerevisiae and was predominantly observed in nuclei. These results suggest that Rbf1p is a transcription factor with telomere-binding activity in C. albicans.
© Society for General Microbiology 1997 | Published by the Microbiology Society
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