1887

Microbiology Society logo

Volume 143, Issue 5

β-Glucosylated proteins in the cell wall of the black yeast Free

Abstract

Wild-type cells of the pathogenic black yeast grown in a low-pH ascorbate medium became less melanized and less resistant to Zymolyase. This was accompanied by increased staining with fluorescently labelled concanavalin A. The sugar composition of wild-type and mutant cell walls was, except for the presence of galactose, similar to that of Digestion of mutant cell walls with laminarinase released galactomannoproteins. In addition, the released cell wall proteins contained glucose and reacted with affinity-purified 1,6-β-glucan antiserum, indicating that they are linked to 1,6-β-glucan. It is proposed that 1,6-β-glucosylated cell wall proteins generally occur among ascomycetes.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 1,6-β-glucan , black yeast , cell wall proteins and galactomannoproteins
© Society Society for General Microbiology 1997
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-5-1673
1997-05-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/5/mic-143-5-1673.html?itemId=/content/journal/micro/10.1099/00221287-143-5-1673&mimeType=html&fmt=ahah

References

  1. Bahl P., Agrawal K. M. L. 1969; Glycosidases of Aspergillus niger. Purification and characterization of α- and β-galactosidases and β-N-acetylglucosaminidase. J Biol Chem 244:2970–2978
     [Google Scholar]
  2. Ballou C.E. 1982; Yeast cell wall and cell surface. In The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression, . Edited by J. N. Strathern, E. W. Jones & J. R. Broach. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. pp:335–360
     [Google Scholar]
  3. Ballou C.E. 1990; Isolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects. Methods Enzymol 185:440–470
     [Google Scholar]
  4. BlaschkeHellmessen R., Lauterbach I., Paul K.-D., Tintelnot K., WeiBbach G. 1994; Isolation of Exophiala dermatitidis (Kano) De Hoog 1977 from a child with septicaemia in acute lymphatic leukaemia and from patients with cystic fibrosis. Mycoses 37:Suppl. 189–96
     [Google Scholar]
  5. Butler M.J., Lachance M. A. 1987; Inhibition of melanin synthesis in the black yeast Phaeococcomyces sp. by growth on low pH ascorbate medium : production of spheroplasts from ‘albinized’ cells. Can J Microbiol 33:184–187
     [Google Scholar]
  6. Crosby J. H., O'Quinn M. H., Steele J. C. H, Rao R. N. 1989; Fine-needle aspiration of subcutaneous phaeohyphomycosis caused by Wangiella dermatitidis . Diagn Cytopathol 5:293–297
     [Google Scholar]
  7. De Hoog G. S., Guarro J.(editors). 1995; Atlas of Clinical Fungi. Baarn/Tarragona : Centraalbureau voor Schimmelcultures/ Universitat Rovira i Virgili..
    [Google Scholar]
  8. De Hoog G. S., Gerrits van den Ende A. H. G., Uijthof J. M. J., Untereiner W. A. 1995; Nutritional physiology of type isolates of currently accepted species of Exophiala and Phaeococcomyces. Antonie Leeuwenhoek 68:43–50
     [Google Scholar]
  9. De Nobel J. G., Dijkers C., Hooijberg E., Klis F. M. 1989; Increased cell wall porosity in Saccharomyces cerevisiae after treatment with dithiothreitol or EDTA. J Gen Microbiol 135:2077–2084
     [Google Scholar]
  10. Dixon D. M., Polak A., Szaniszlo P. J. 1987; Pathogenicity and virulence of wild-type and melanin-deficient Wangiella dermatitidis. J Med Vet Mycol 25:97–106
     [Google Scholar]
  11. Dixon D. M., Polak A., Conner G. W. 1989; mel - mutants of Wangiella dermatitidis in mice : evaluation of multiple mouse and fungal strains. J Med Vet Mycol 27:335–341
     [Google Scholar]
  12. Dixon D. M., Szaniszlo P. J., Polak A. 1991; Dihydroxynaphthalene (DHN) melanin and its relationship with virulence in the early stages of phaeohyphomycosis. In The Fungal Spore and Disease Initiation in Plants and Animals,. Edited by G. T. Cole & H. C. Hoch. New York: Plenum Press. pp:335–341
     [Google Scholar]
  13. Dixon D. M., Migliozzi J., Cooper C. R. Jr, Solis O., Breslin B., Szaniszlo P.J. 1992; Melanized and non-melanized multicellular form mutants of Wangiella dermatitidis in mice: mortality and histopathology studies. Mycoses pp:335–341
     [Google Scholar]
  14. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
     [Google Scholar]
  15. Fleet G.H. 1991; Cell walls. In The Yeasts, 2nd edn, vol. 4, Yeast Organelles,. Edited by A. H. Rose & J. S . Harrison. London : Academic Press. pp:199–277
     [Google Scholar]
  16. Frevert J., Ballou C. E. 1985; S. cerevisiae structural cell wall mannoproteins. Biochemistry 24:753–759
     [Google Scholar]
  17. Geis P. A. 1981; Chemical composition of the yeast and sclerotic cell walls of Wangiella dermatitidis. PhD thesis, University of Texas.
     [Google Scholar]
  18. Geis P. A., Szaniszlo P. J. 1984; Carotenoid pigments of the dematiaceous fungus Wangiella dermatitidis. Mycologia 76:268–273
     [Google Scholar]
  19. Haase G., Skopnik H., Groten T., Kusenbach G., Posselt H. G. 1991; Long-term fungal cultures from patients with cystic fibrosis. Mycoses 34:373–376
     [Google Scholar]
  20. Hard K., Bitter W., Kamerling J. P., Vliegenthart J. F. G. 1989; O-Mannosylation of recombinant human insulin-like growth factor I (IGF-I) produced in Saccharomyces cerevisiae. FEBS Lett 248:111–114
     [Google Scholar]
  21. Herscovics A., Orlean P. 1993; Glycoprotein biosynthesis in yeast. FASEB J 7:540–550
     [Google Scholar]
  22. Hiruma M., Kawada A., Ohata T., Ohnishi Y., Takahashi H., Yamazaki M., Ishibashi A., Hatsuse K., Kakihara M., Yoshida M. 1993; Systemic phaeohyphomycosis caused by Exophiala dermatitidis. Mycoses 36:1–7
     [Google Scholar]
  23. Hohl P.E., Holley H.P., Prevost E., Ajello L., Padhye A. A. 1983; Infections due to Wangiella dermatitidis in humans. Rev Infect Dis 5:854–864
     [Google Scholar]
  24. Kabel P. J., Illy K. E., Holl R. A., Buiting A. G. M., Wintermans R. G. F. 1994; Nosocomial intravascular infection with Exophiala dermatitidis. Lancet 344:1167–1168
     [Google Scholar]
  25. Kapteyn J. C., Montijn R. C., Dijkgraaf G. J. P., Klis F. M. 1994; Identification of β-1,6-glucosylated cell wall proteins in yeast and hyphal forms of Candida albicans. Eur J Cell Biol 65:402–407
     [Google Scholar]
  26. Kapteyn J. C., Montijn R. C., Dijkgraaf G. J. P., Van Den Ende H., Klis F. M. 1995; Covalent association of β-1,3-glucan with β- 1,6-glucosylated mannoproteins in cell walls of Candida albicans. J Bacteriol 177:3788–3792
     [Google Scholar]
  27. Kapteyn J. C., Montijn R. C., Vink E., Van Den Ende H., De La Cruz J., Llobell A., Douwes J. E., Shimoi H., Lipke P. N., Klis F. M. 1996; Retention of Saccharomyces cerevisiae cell wall proteins through a phosphodiester-linked β-1,3-/β-1,6-glucan heteropolymer. Glycobiology 6:337–345
     [Google Scholar]
  28. Klis F. M. 1994; Cell wall assembly in yeast. Yeast 10:851–869
     [Google Scholar]
  29. Kukuruzinska M. A., Bergh M. L E., Jackson B. J. 1987; Protein glycosylation in yeast. Annu Rev Biochem 56:915–944
     [Google Scholar]
  30. Kusenbach G., Skopnik H., Haase G., Friedrichs F., Doehmen H. 1992; Exophiala dermatitidis pneumonia in cystic fibrosis. Eur J Pediatr 151:344–346
     [Google Scholar]
  31. Kwon-Chung K. J., Bennett J. W. 1992; Philadelphia : Lea & Febiger. Medical Mycology
     [Google Scholar]
  32. Lu C. F., Kurjan J., Lipke P. N. 1994; A pathway for cell wall anchorage of Saccharomyces cerevisiae α-agglutinin. Mol Cell Biol 14:4825–4833
     [Google Scholar]
  33. Lu C. F., Montijn R. C., Brown J. L., Klis F. M., Kurjan J., Bussey H., Lipke P.N. 1995; Glycosyl phosphatidylinositol-dependent cross-linking of α-agglutinin and β-l,6-glucan in the Saccharomyces cerevisiae cell wall. J Cell Biol 128:333–340
     [Google Scholar]
  34. Matsumoto T., Padhye A.A., Ajello L., Standard P. G., McGinnis M. R. 1984; Critical review of human isolates of Wangiella dermatitidis. Mycologia 76:232–249
     [Google Scholar]
  35. Matsumoto T., Matsuda T., McGinnis M. R., Ajello L. 1993; Clinical and mycological spectra of Wangiella dermatitidis infections. Mycoses 36:145–155
     [Google Scholar]
  36. McMurrough I., Rose A. H. 1967; Effect of growth rate and substrate limitation on the composition and structure of the cell wall of Saccharomyces cerevisiae. Biochem J 105:189–203
     [Google Scholar]
  37. Montijn R. C., van Rinsum J., van Schagen F. A., Klis F. M. 1994; Glucomannoproteins in the cell wall of Saccharomyces cerevisiae contain a novel type of carbohydrate side chain. J Biol chem 269:19338–19342
     [Google Scholar]
  38. Pastor F. I. J., Valentin E., Herrero E., Sentandreu R. 1984; Structure of the S. cerevisiae cell wall : mannoproteins released by zymolyase and their contributions to wall architecture. Biochim Biophys Acta 802:292–300
     [Google Scholar]
  39. Polak A. 1989; Melanin as a virulence factor in pathogenic fungi. Mycoses 33:215–224
     [Google Scholar]
  40. Polak A., Dixon D. M. 1993; Pathogenicity of Wangiella dermatitidis. In Dimorphic Fungi in Biology and Medicine,. Edited by H. Van den Bossche, F. C. Odds & D. Kerridge. New York: Plenum Press. pp:307–312
     [Google Scholar]
  41. Pringle J. R., Preston R. A., Adams A. E. M, Stearns T., Drubin D. G., Haarer B. K., Jones E. W. 1989; Fluorescence microscopy methods for yeast. Methods Cell Bio 31:357–435
     [Google Scholar]
  42. Roemer T., Delaney S., Bussey H. 1993; SKN1 and KRE6 define a pair of functional homologs encoding putative membrane proteins involved in β-glucan synthesis. Mol Cell Biol 13:4039–4048
     [Google Scholar]
  43. Schoffelmeer E., Kapteyn J. C., Montijn R.C., Cornelissen B.C., Klis F.M. 1996; Glucosylation of fungal cell wall proteins as a potential target for novel anti-fungal agents. In Modern Fungicides and Antifungal Compounds. Edited by H. Lyr, P. E. Russel & H. D. Sisler. Hampshire, UK: Intercept..
    [Google Scholar]
  44. Schreuder M. P., Brekelmans S., Van Den Ende H., Klis F.M. 1993; Targeting of a heterologous protein to the cell wall of Saccharomyces cereuisiae. Yeast 9:399–409
     [Google Scholar]
  45. Szaniszlo P. J., Geis P. A., Jacobs C. W., Cooper C. R., Harris J.L. 1983; Cell wall changes associated with yeast-tomulticellular form conversion in Wangiella dermatitidis. In Microbiology - 1983, Edited by D. Schlessinger. Washington, DC : American Society for Microbiology.. pp:239–244
     [Google Scholar]
  46. Taylor B. E., Wheeler M. H., Szaniszlo P. J. 1987; Evidence for pentaketide melanin biosynthesis in dematiaceous human pathogenic fungi. Mycology 79:320–322
     [Google Scholar]
  47. Tkacz J. S., Cybulska B., Lampen J. O. 1971; Specific staining of wall mannan in yeast cells with fluorescein-conjugated concanavalin A. J Bacteriol 105:1–5
     [Google Scholar]
  48. Uijthof J. M. J., De Hoog G. S., De Cock A. W. A. M., Takeo K., Nishimura K. 1994; Pathogenicity of strains of the black yeast Exophiala (Wangiella) dermatitidis : an evaluation based on polymerase chain reaction. Mycoses 37:235–242
     [Google Scholar]
  49. Van Berkel M. A. A., Caro L. H. P., Montijn R. C., Klis F. M. 1994; Glucosylation of chimeric proteins in the cell wall of Saccharomyces cerevisiae. FEBS Lett 349:135–138
     [Google Scholar]
  50. Van Der Vaart J. M., Caro L. H. P., Chapman J. W., Klis F. M., Verrips C. T. 1995; Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae. J Bacteriol 177:3104–3110
     [Google Scholar]
  51. Van Rinsum J., Klis F. M., Van den Ende H. 1991; Cell wall glucomannoproteins of Saccharomyces cerevisiae mnn9. Yeast 7:717–726
     [Google Scholar]
  52. Zueco J., Mormeneo S., Sentandreu R. 1986; Temporal aspects of the O-glycosylation of S. cerevisiae mannoproteins. Biochim Biophys Acta 248:93–100
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-5-1673
Loading
β-Glucosylated proteins in the cell wall of the black yeast Exophiala (Wangiella) dermatitidis
Microbiology 143, 1673 (1997); https://doi.org/10.1099/00221287-143-5-1673
/content/journal/micro/10.1099/00221287-143-5-1673
/content/journal/micro/10.1099/00221287-143-5-1673
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error