1887

Microbiology Society logo

Volume 143, Issue 6

Identification of a phenolic 3-methyltransferase in the lignin-degrading fungus Free

Abstract

A methyl transferase enzyme catalysing the 3--methylation of isovanillic acid (3-hydroxy-4-methoxy!benzoic acid) by -adenosylmethionine (SAM) was identified in and purified. Gel filtration indicated an of 71000 and SDS-PAGE showed that the enzyme was composed of two sublimits of M approximately 36000. Substrate utilization studies demonstrated that the enzyme was highly specific, displaying an exclusive preference for the methylation of the 3-hydroxyl group of several substituted benzoic acids. 3-Hydroxybenzoic acids with a methoxyl or hydroxyl substituent in the 2 or 4 position were the best substrates with isovanillic and 3,4-dihydroxybenzoic acids showing the highest rates of methylation. The 3--methyltransferase enzyme was induced later in the growth cycle than the 4--methyltransferase previously isolated from this fungus, which is believed to have a role in the 4--methylation of lignin degradation products. However the function of this -specific enzyme, the first phenolic 3--methyltransferase isolated from a fungus, remains unclear. The combined activities of the 3- and 4--methyltransferase enzymes satisfactorily account for the pattern of SAM-dependent methylating activity shown by whole mycelia to phenolic substrates.

  • Published Online:
Keyword(s): lignin degradation , O-methyltransferase , Phanerochaete chrysosporium , phenolic acids and S-adenosylmethionine
© Society Society for General Microbiology 1997
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-6-1975
1997-06-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/6/mic-143-6-1975.html?itemId=/content/journal/micro/10.1099/00221287-143-6-1975&mimeType=html&fmt=ahah

References

  1. Chen C.L., Chang H.M., Kirk T.K. 1982; Aromatic acids produced during degradation of lignin in spruce wood by Phanerochaete chrysosporium.. Holzforschung 36:3–9
     [Google Scholar]
  2. Coulter C., Hamilton J.T.G., Harper D.B. 1983a; Evidence for the existence of independent chloromethane and S-adenosylmethionine- utilizing systems for methylation in hanerochaete chrysosporium.. Appl Environ Microbiol 59:1461–1466
     [Google Scholar]
  3. Coulter C., Kennedy J.T., McRoberts D.B., Harper D.B. 1983a; Purification and properties of an S-adenosylmethionine 2,4-disubstituted phenol O-methyltransferase from Phanerochaete chrysosporium . Appl Environ Microbiol 59:706–711
     [Google Scholar]
  4. Dixon M. 1953; Determination of enzyme inhibitor constants. Biochem J 55:170–171
     [Google Scholar]
  5. Dixon M., Webb E.C. 1979; The Enzymes, 3rd edn. London. Longman.82–87
     [Google Scholar]
  6. Eriksson K.-E., Gupta J.K., Nishida A., Rao R. 1984; Syringic acid metabolism by some white rot, soft rot and brown rot fungi.. J Gen Microbiol 130:2457–2464
     [Google Scholar]
  7. Harper D.B., Hamilton J.T.G., Kennedy J.T., McNally K.J. 1989; Chloromethane, a novel methyl donor for biosynthesis of esters and anisoles in Phellinus pomaceus . J Gen Microbiol 134:2831–2839
     [Google Scholar]
  8. Harper D.B., Buswell J.A., Kennedy J.T., Hamilton J.T.G. 1990; Chloromethane, methyl donor in veratryl alcohol biosynthesis in Phanerochaete chrysosporium and other lignin degrading fungi. Appl Environ Microbiol 56:3450–3457
     [Google Scholar]
  9. Harper D.B., Buswell J.A., Kennedy J.T. 1991; Effect of chloromethane on veratryl alcohol and lignin peroxidase production by the fungus Phanerochaete chrysosporium.. J Gen Microbiol 137:2867–2872
     [Google Scholar]
  10. Harper D.B., McRoberts W.C., Kennedy J.T. 1996; Comparison of the efficacies of chloromethane, methionine and Sadenosylmethionine as methyl precursors in the biosynthesis of veratryl alcohol and related compounds in Phanerochaete chrysosporium.. Appl Environ Microbiol 62:3366–3370
     [Google Scholar]
  11. Jensen K. A. Jr, Evans K. M. C., Kirk T.K., Hammel K.E. 1994; Biosynthetic pathway for veratryl alcohol in the ligninolytic fungus Phanerochaete chrysosporium.. Appl Environ Microbiol 60:709–714
     [Google Scholar]
  12. Poulton J.E. 1981; Transmethylation and demethylation reactions in the metabolism of secondary plant products. In The Biochemistry of Plants. Edited by P. K. Stumpf % E. E. Conn. London. Academic Press. 7:667–723
     [Google Scholar]
  13. Valli K., Gold M.H. 1991; Degradation of 2,4-dichlorophenol by the lignin-degrading fungus Phanerochaete chrysosporium.. J Bacteriol 173:345–352
     [Google Scholar]
  14. Valli K., Wariishi H., Gold M.H. 1992; Degradation of 2,7- dichlorodibenzo-P-dioxin by the lignin-degrading basidiomycete Phanerochnete chrysosporium.. J Bacteriol 174:2131–2137
     [Google Scholar]
  15. Wat C.K., Towers G. H. N. 1975; Phenolic O-methyltransferases from Lentinus lepideus (Basidiomycete). Phytochemistry 14:663–666
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-143-6-1975
Loading
Identification of a phenolic 3-O-methyltransferase in the lignin-degrading fungus Phanerochaete chrysosporium
Microbiology 143, 1975 (1997); https://doi.org/10.1099/00221287-143-6-1975
/content/journal/micro/10.1099/00221287-143-6-1975
/content/journal/micro/10.1099/00221287-143-6-1975
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error