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Volume 144, Issue 4

A 35.7 kb DNA fragment from the chromosome containing a putative 12.3 kb operon involved in hexuronate catabolism and a perfectly symmetrical hypothetical catabolite-responsive element Free

Abstract

The strain 168 chromosomal region extending from 109† to 112° has been sequenced. Among the 35 ORFs identified, and were the only genes that had been previously mapped and sequenced. Out of ten ORFs belonging to a single putative transcription unit, seven are probably involved in hexuronate catabolism. Their sequences are homologous to genes and , which are all required for the uptake of free -glucuronate, -galacturonate and β-glucuronide, and their transformation into glyceraldehyde 3-phosphate and pyruvate via 2-keto-3-deoxygluconate. The remaining three ORFs encode two dehydrogenases and a transcriptional regulator. The operon is preceded by a putative catabolite-responsive element (CRE), located between a hypothetical promoter and the RBS of the first gene. This element, the longest and the only so far described that is fully symmetrical, consists of a 26 bp palindrome matching the theoretical CRE sequence. The remaining predicted amino acid sequences that share homologies with other proteins comprise: a cytochrome P-450, a glycosyltransferase, an ATP-binding cassette transporter, a protein similar to the formate dehydrogenase α-subunit (FdhA), a protein similar to NADH dehydrogenases, and three homologues of polypeptides that have undefined functions.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Bacillus subtilis , catabolite repression , genome sequencing and hexuronate catabolism
© Society for General Microbiology 1998
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1998-04-01
2024-04-26
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A 35.7 kb DNA fragment from the Bacillus subtilis chromosome containing a putative 12.3 kb operon involved in hexuronate catabolism and a perfectly symmetrical hypothetical catabolite-responsive element
Microbiology 144, 877 (1998); https://doi.org/10.1099/00221287-144-4-877
/content/journal/micro/10.1099/00221287-144-4-877
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