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Volume 144, Issue 4

The -acetylmuramoyl-L-alanine amidase encoded by the 168 prophage SPβ Free

Abstract

Heat shock of CU1147, a strain lysogenic for SPβc2, a prophage with a thermosensitive repressor, results in phage induction and subsequent cell lysis. Cloning in and sequencing of a DNA fragment of prophage SPβ led to the identification of , the gene encoding a 367 amino acid polypetide with a molecular mass of 39.6 kDa. Purified BlyA obtained from the clone exhibited an -acetylmuramoyl-L-alanine amidase activity. Insertional mutagenesis confirmed that the latter enzyme was associated with SPβ-phage-mediated ceil lysis. Analysis of the neighbouring sequence suggested that the two ORFs immediately downstream of and belonging to the same operon encode polypeptides which may be involved in the release of the endolysin. The presence on the chromosomes of B. subtilis or related . of other, similar genes, and their possible relationship, is discussed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Bacillus subtilis phage , endolysin , holin , N-acetylmuramoyl-L-alanine amidase and prophage SPβ
© Society for General Microbiology 1998
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1998-04-01
2024-04-28
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The N-acetylmuramoyl-L-alanine amidase encoded by the Bacillus subtilis 168 prophage SPβ
Microbiology 144, 885 (1998); https://doi.org/10.1099/00221287-144-4-885
/content/journal/micro/10.1099/00221287-144-4-885
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