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Volume 144, Issue 6

Phytase activity of anaerobic ruminal bacteria Free

Abstract

Phytase catalyses the release of phosphate from phytate (-inositol hexakisphosphate), the predominant form of phosphorus in cereal grains, oilseeds and legumes. The presence of phytase activity was investigated in 334 strains of 22 species of obligately anaerobic ruminal bacteria. Measurable activities were demonstrated in strains of spp. Strains isolated from fermentations with cereal grains proved to have high activity, and activity was particularly prevalent in with over 96% of the tested strains being positive. The measured phytase activity was found exclusively associated with the bacterial cells and was produced in the presence of approximately 14 mM phosphate. The most highly active strains were all , with the exception of the one strain examined. Phytase activity varied greatly among positive strains but activities as high as 703 nmol phosphate released (ml culture) were measured for a strain and 387 nmol phosphate released (ml culture) for the strain.

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  • Published Online:
Keyword(s): anaerobic , phosphate , phytase , rumen and Selenomonas,
© Society Society for General Microbiology, 1998
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1998-06-01
2024-04-30
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References

  1. Al-Asheh, S., Duvnjak, Z. (1995); The effect of phosphate concentration on phytase production and the reduction of phytic acid content in canola meal by Aspergillus carbonarius during a solid-state fermentation process.. Appl Microbiol Biotecbnol 43,:25–30
     [Google Scholar]
  2. Bonhomme, A. (1990); Rumen ciliates: their metabolism and relationships with bacteria and their hosts.. Anim Feed Sci Technol, 30,:203–266
     [Google Scholar]
  3. Broz, J., Oldale, P., Perrin-Voltz, A. H., Rychen, G., Schulze, J., Simoes Nunes, C. (1994); Effects of supplemental phytase on performance and phosphorus utilisation in broiler chickens fed a low phosphorus diet without addition of inorganic phosphates.. Br J Poultry Sci, 35,:273–280
     [Google Scholar]
  4. Bryant, M. P., Burkey, L. (1953); Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen.. Journal of Dairy Science, 36,:205–217
     [Google Scholar]
  5. Cheng, E. W., Hall, G., Burroughs, W. (1955); A method for the study of cellulose digestion by washed suspensions of rumen microorganisms.. Journal of Dairy Science, 38,:1255–1230
     [Google Scholar]
  6. Common, F. H. (1989); Biological availability of phosphorus for μlgs.. Nature, 143,:370–380
     [Google Scholar]
  7. Czerkawski, J. W., Breckenridge, G. (1977); Design and development of a long-term rumen simulation technique (Rusitec).. Br J Nutr, 38,:371–384
     [Google Scholar]
  8. Durand, M., Kawashima R. (1980) Influence of minerals in rumen microbial digestion.. Edited by Ruckebusch Y., Thivend. P. Digestive Physiology and Metabolism in Ruminants. Lancaster, UK:: MTP Press,;375M08
     [Google Scholar]
  9. Gibson, D. M., Ullah A. B. J. (1988); Purification and characterization of phytase from cotyledons of germinating soybean seeds.. Archives of Biochemistry and Biophysics, 260,:503–513
     [Google Scholar]
  10. Greiner, R., Konietzny, U., Jany, K.-D. (1993); Purification and characterization of two phytases from Escherichia coli.. Archives of Biochemistry and Biophysics, 303,:107–113
     [Google Scholar]
  11. Hubei, F., Beck, E. (1996); Maize root phytase: purification, characterization, and localization of enzyme activity and its putative substrate.. Plant Physiology, 112,:1429–1436
     [Google Scholar]
  12. Hungate, R. E. (1950); The anaerobic mesophilic cellulolytic bacteria.. Bacteriological Reviews, 14,:1–49
     [Google Scholar]
  13. McAllister, T. A., Rode, L. M., Cheng, K.-J., Buchanan-Smith, J. G. (1992); Effect of formaldehyde-treated barley or escape protein on the ruminal environment and digestion in steers.. Can ] Anim Sci, 72,:317–328
     [Google Scholar]
  14. McAllister, T. A., Phillippe, R. C., Rode, L. M., Cheng, K.-J. (1993); Effect of the protein matrix on the digestion of cereal grains by ruminal microorganisms.. Journal of Animal Science, 71,:205–212
     [Google Scholar]
  15. McAllister, T. A., Bae, H. D., Jones, G. A., Cheng K.-J. (1994); Microbial attachment and feed digestion in the rumen.. ] Anim Sci, 72,:3004–3018
     [Google Scholar]
  16. Maga, J. A. (1982); Phytate: its chemistry, occurrence, food interactions, nutritional significance, and methods of analysis.. J Agric Food Chem, 30,:1–9
     [Google Scholar]
  17. Mitchell, D. B., Vogel, K., Weimann, B. J., Pasamontes, L., Van Loon A. P. G. M. (1997); The phytase subfamily of histidine phosphatases: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila.. Microbiology, 143,:242–252
     [Google Scholar]
  18. Pen, J., Verwoerd, T. C., Van Paridon, P. A., Beudeker, R. F. (1993) 8c 7 other authors, Phytase-containing transgenic seeds as a novel feed additive for improved phosphorus utilization.. Bio/ Technology, 11,:811–814
     [Google Scholar]
  19. Raun, A., Cheng, E., Burroughs, W. (1956); Phytate phosphorus hydrolysis and availability to rumen microorganisms.. Agric Food Chem, 4,:869–871
     [Google Scholar]
  20. Reddy, N. R., Sathe, S. K., Salunkhe D. K. (1982) Phytates in legumes and cereals.. Edited by Chichester, C. O., Mrak, E. M., Stewart, G. F. Advances in Food Chemistry. New York:: Academic Press,;1–92
     [Google Scholar]
  21. Ricke, S. C., Martin, S. A., Nisbet D. J. (1996); Ecology, metabolism, and genetics of ruminal selenomonads.. Crc Critical Reviews in Microbiology, 22,:27–65
     [Google Scholar]
  22. Satoh, E., Niimura, Y., Uchimura, T., Kozaki, M., Komagata K. (1993); Molecular cloning and expression of two a-amylases from Streptococcus bovis 148 in Escherichia coli.. Appl Environ Microbiol, 59,:3669–3673
     [Google Scholar]
  23. Scott, H. W., Dehority B. A. (1965); Vitamin requirements of several cellulolytic bacteria.. Journal of Bacteriology, 89,:1169–1175
     [Google Scholar]
  24. Segueilha, L., Moulin, G., Galzy P. (1993); Reduction of phytate content in wheat bran and glandless cotton flour by Schwan- niomyces castellii.. J Agric Food Chem, 41,:2451–2454
     [Google Scholar]
  25. Shimizu, M. (1992); Purification and characterization of phytase from Bacillus subtilis (natto) N-77.. Biosci Biotechnol Biochem, 56,:1266–1269
     [Google Scholar]
  26. Shimizu, M. (1993); Purification and characterization of phytase and acid phosphatase produced by Aspergillus oryzae Kl.. Biosci Biotechnol Biochem, 57,:1364–1365
     [Google Scholar]
  27. Stewart, C. S., Bryant M. P. (1988) The rumen bacteria.. Edited by Hobson, P. N. The Rumen Microbial Ecosystem. London:: Elsevier Applied Science,;21–76
     [Google Scholar]
  28. Teunissen, M. J., Op den Camp H. J. M. (1993); Anaerobic fungi and their cellulolytic and xylanolytic enzymes.. Antonie Leeuwenhoek, 63,:63–76
     [Google Scholar]
  29. Ullah, A. H. J. (1988a); Production, raμld purification and catalytic characterization of extracellular phytase from Aspergillus ficuum.. Preparative Biochemistry, 18,:443–458
     [Google Scholar]
  30. Ullah, A. H. J. (1988b); Aspergillus ficuum phytase: partial primary structure, substrate selectivity, and kinetic characterization.. Preparative Biochemistry, 18,:459–471
     [Google Scholar]
  31. Van der Kaay J., Van Haastert P. J. M. (1995); Stereospecificity of inositol hexakisphosphate dephosphorylation by Paramecium phytase.. Biochemical Journal, 312,:907–910
     [Google Scholar]
  32. Van Hartingsveldt, W., Van Zeijl, C. M. J., Harteveld, G. M. (1993) 8 other authors, Cloning, characterization and over- expression of the phytase-encoding gene (phyA) of Aspergillus niger.. Gene, 127,:87–94
     [Google Scholar]
  33. Volfova, 0., Dvorakova, J., Hanzlfkova, A., Jandera, A. (1994); Phytase from Aspergillus niger.. Folia Microbiologica, 39,:481–484
     [Google Scholar]
  34. Wallace, R. J., Cotta, M. A. (1988) Metabolism of nitrogen- containing compounds.. Edited by Hobson, P. N. The Rumen Microbial Ecosystem. London:: Elsevier Applied Science,;217–249
     [Google Scholar]
  35. Yoon, S. J., Choi, Y. J., Min, H. K., Cho, K. K., Kim, J. W., Lee, S. C., Jung, Y. H. (1996); Isolation and identification of phytase- producing bacterium, Enterobacter sp. 4, and enzymatic properties of phytase enzyme.. Enzyme Microb Technol, 18,:449–154
     [Google Scholar]
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Phytase activity of anaerobic ruminal bacteria
Microbiology 144, 1565 (1998); https://doi.org/10.1099/00221287-144-6-1565
/content/journal/micro/10.1099/00221287-144-6-1565
/content/journal/micro/10.1099/00221287-144-6-1565
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