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Volume 146, Issue 1

An unusual pectate lyase from a sp. with high activity on pectin: cloning and characterization

The EMBL accession number for the nucleotide sequence determined in this work is AJ237980.

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Abstract

The gene encoding a pectate lyase from the strain sp. BP-23 was cloned and expressed in . The nucleotide sequence of a 1214 bp DNA fragment containing gene was determined, revealing an ORF of 666 nucleotides that encoded a protein of 23233 Da. The deduced amino acid sequence of the encoded enzyme showed homology to pectate lyases A, B, C and D from , Pel-3 and PelB from and PelI from . Homology was also found to the protein deduced from the gene, the function of which is unknown. The heterologous expressed enzyme depolymerized polygalacturonate and pectins of methyl esterification degree from 22 to 89%, and exhibited similar activity on polygalacturonate and on 89% esterified citrus pectin. Optimum temperature and pH for enzymic activity were 50 °C and pH 10, respectively. Ca was required for activity on pectic substrates, while the enzyme was strongly inhibited by Ba.

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Keyword(s): lyase , pectate and pectin
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2000-01-01
2024-05-02
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An unusual pectate lyase from a Bacillus sp. with high activity on pectin: cloning and characterizationThe EMBL accession number for the nucleotide sequence determined in this work is AJ237980.
Microbiology 146, 89 (2000); https://doi.org/10.1099/00221287-146-1-89
/content/journal/micro/10.1099/00221287-146-1-89
/content/journal/micro/10.1099/00221287-146-1-89
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