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Volume 146, Issue 11

Identification of DNA-binding proteins involved in regulation of expression of the gene, which encodes sporulation sigma factor σ Free

Abstract

Expression of the gene encoding a sporulation-specific sigma factor, σ, in is restricted only to sporulation. Gel mobility-shift assays using protein fractions from different developmental stages of revealed two different putative proteins specifically bound to the promoter region: a protein (designated RsfA) present in young substrate mycelium, and a protein (designated RsfB) present in the course of sporulation. Based on the characteristic profiles of their appearance during differentiation, RsfA might be a repressor and RsfB an activator of expression. The location of a specific binding site of the repressor-like protein (RsfA) was determined by gel mobility-shift assays of promoter deletion fragments and by DNase I footprinting analysis. The binding site mapped from nucleotides −87 to −25 relative to the transcription start point of the promoter, and overlapped the −35 promoter region. Given the dependence of expression upon , the putative sporulation transcription factor WhiH was overproduced in and used in the mobility-shift assays with the promoter. However, no specific binding was detected, indicating an indirect dependence of upon .

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  • Published Online:
Keyword(s): differentiation , promoter , repressor , sigma factor , Streptomyces aureofaciens and tsp, transcription start point
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2000-11-01
2024-05-02
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Identification of DNA-binding proteins involved in regulation of expression of the Streptomyces aureofaciens sigF gene, which encodes sporulation sigma factor σF
Microbiology 146, 2919 (2000); https://doi.org/10.1099/00221287-146-11-2919
/content/journal/micro/10.1099/00221287-146-11-2919
/content/journal/micro/10.1099/00221287-146-11-2919
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