1887

Microbiology Society logo

Volume 146, Issue 9

Inhibition of chromosome replication in : effect of the promoter region Free

Abstract

In a previous study a functional mycobacterial origin of replication, , was isolated on a plasmid. However, it was found that origin function was inhibited by the presence of the adjacent gene or its regulatory region, so that plasmids containing both of these regions next to the origin did not yield transformants. This inhibition could be due either to overexpression of on a plasmid being toxic, the transcription of into the downstream origin topologically inhibiting its function, or to the DnaA boxes upstream of somehow interacting with the DnaA boxes in the origin to prevent its function. To distinguish between these possibilities, plasmids were constructed lacking different parts of the gene: the promoter, the DnaA boxes, or both. Additionally, the putative promoter region was replaced by mycobacterial sequences that exhibit weaker or null promoter activity. The results indicate that the promoter region, but not the DnaA boxes, is the principal cause of the incompatibility observed and suggest that this region could be playing a role in the inhibition of chromosome replication.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): dnaA promoter , FIS, factor for inversion stimulation , GFP, green fluorescent protein , IHF, integration host factor protein , incompatibility , Mycobacterium , OADC, oleic acid albumin dextrose complex and oriC/replication
© Microbiology Society
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-146-9-2199
2000-09-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/146/9/1462199a.html?itemId=/content/journal/micro/10.1099/00221287-146-9-2199&mimeType=html&fmt=ahah

References

  1. Asai T., Chen C. P., Nagata T., Takanami M., Imai M. 1992; Transcription in vivo within the replication origin of the Escherichia coli chromosome: a mechanism for activating initiation of replication. Mol Gen Genet 231:169–178
     [Google Scholar]
  2. Atlung T., Clausen E., Hansen F. G. 1985; Autoregulation of the dnaA gene of Escherichia coli. Mol Gen Genet 200:442–450 [CrossRef]
     [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Siedman J. G., Smith J. A., Sthrul K. 1995 Current Protocols in Molecular Biology New York: Green Publishing Associates/Wiley-Interscience;
     [Google Scholar]
  4. Baker T. A., Funnell B. E., Kornberg A. 1987; Helicase action of DnaB protein during replication from the Escherichia coli chromosomal origin in vitro. J Biol Chem 262:6877–6885
     [Google Scholar]
  5. Bramhill D., Kornberg A. 1988; A model for initiation at origins of DNA replication. Cell 54:915–918 [CrossRef]
     [Google Scholar]
  6. Casart Y. 1996 Efecto del relajamiento del ADN sobre la expresión del promotor de los genes de la ADN girasa de Mycobacterium tuberculosis Pre-doctorate thesis IVIC; Venezuela: pp. 36–40
     [Google Scholar]
  7. Crook E., Hwanmg D. S., Sharstad K., Thöny B., Kornberg A. 1993; E. coli minichromosome replication: regulation of initiation at oriC. Res Microbiol 142:127–130
     [Google Scholar]
  8. Dolin P. J., Raviglione M. C., Kochi A. 1994; Global tuberculosis incidence and mortality during 1990–2000. Bull WHO 72:213–220
     [Google Scholar]
  9. Jacobs W. R., Kalpana G. V., Cirillo J. D., Pascospella L., Snapper S. B., Udani R. A., Jones W., Barletta R. G., Bloom B. R. 1991; Genetic systems in mycobacteria. Methods Enzymol 204:537–555
     [Google Scholar]
  10. Jakimowicz D., Majka J., Messer W.8 other authors 1998; Structural elements of the Streptomyces oriC region and their interactions with DnaA protein. Microbiology 144:1281–1290 [CrossRef]
     [Google Scholar]
  11. Krause M., Rückert B., Lurz R., Messer W. 1997; Complexes at the replication origin of Bacillus subtilis with homologous and heterologous DnaA protein. J Mol Biol 274:365–380 [CrossRef]
     [Google Scholar]
  12. Madiraju M., Qin M. H., Yamamoto K., Atkinson M., Rajagopalan M. 1999; The dnaA gene region of Mycobacterium avium and the autonomous replication activities of its 5′ and 3′ flanking regions. Microbiology 145:2913–2921
     [Google Scholar]
  13. Marszalek J., Kaguni J. M. 1994; DnaA protein directs the binding of DnaB protein in initiation of DNA replication in Escherichia coli. J Biol Chem 269:4883–4890
     [Google Scholar]
  14. von Meyenburg K., Hansen F. G. 1987; Regulation of chromosome replication. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp 1555–1577Edited by Neidhardt F. C.others Washington, DC: American Society for Microbiology;
     [Google Scholar]
  15. Moriya S., Fukuoka T., Ogasawara N., Yoshikawa H. 1988; Regulation of initiation of the chromosomal replication by DnaA-boxes in the origin of the Bacillus subtilis chromosome. EMBO J 7:2911–2917
     [Google Scholar]
  16. Moriya S., Kato K., Yoshikawa H., Ogasawara N. 1990; Isolation of a dnaA mutant of Bacillus subtilis defective in initiation of replication: amount of DnaA protein determines cells’ initiation potential. EMBO J 9:2905–2910
     [Google Scholar]
  17. Moriya S., Atlung T., Hansen F. G., Yoshikawa H., Ogasawara N. 1992; Cloning of an autonomously replicating sequence (ars) from Bacillus subtilis chromosome. Mol Microbiol 6:309–315 [CrossRef]
     [Google Scholar]
  18. Moriya S., Imai Y., Hassan A. K. M., Ogasawara N. 1999; Regulation of initiation of Bacillus subtilis chromosome replication. Plasmid 41:17–29 [CrossRef]
     [Google Scholar]
  19. Ogasawara N., Moriya S., Yoshikawa H. 1991; Initiation of chromosome replication: structure and function of oriC and DnaA protein in eubacteria. Res Microbiol 142:851–859 [CrossRef]
     [Google Scholar]
  20. Polaczek P. 1998; Is the dnaA promoter region in Escherichia coli an evolutionary junkyard of physiologically insignificant regulatory elements?. Mol Microbiol 27:1089–1090 [CrossRef]
     [Google Scholar]
  21. Polaczek P., Wright A. 1990; Regulation of expression of the dnaA gene in Escherichia coli: role of the two promoters and the DnaA box. New Biol 2:574–582
     [Google Scholar]
  22. Roth A., Urmoneit B., Messer A. 1994; Functions of histone-like proteins in initiation of DNA replication at oriC of Escherichia coli. Biochimie 76:917–923 [CrossRef]
     [Google Scholar]
  23. Qin M. H., Madiraju M., Zachariah S., Rajagopalan M. 1997; Characterization of the oriC region of Mycobacterium smegmatis. J Bacteriol 179:6311–6317
     [Google Scholar]
  24. Qin M. H., Madiraju M., Rajagopalan M. 1999; Characterization of the functional replication origin of Mycobacterium tuberculosis. Gene 233:121–130 [CrossRef]
     [Google Scholar]
  25. Salazar L., Fsihi H., de Rossi E., Riccardi G., Rios C., Cole S. T., Takiff H. E. 1996; Organization of the origins of replication of the chromosome of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatis. Mol Microbiol 20:283–293 [CrossRef]
     [Google Scholar]
  26. Sekimizu K., Yung B. Y., Kornberg A. 1988; The DnaA protein of Escherichia coli: abundant, improved purification and membrane binding. J Biol Chem 263:7136–7140
     [Google Scholar]
  27. Speck C., Weigel C., Messer W. 1999; ATP- and ADP-DnaA protein, a molecular switch in gene regulation. EMBO J 18:6169–6176 [CrossRef]
     [Google Scholar]
  28. Timm J., Lim E. M., Gicquel B. 1994; Escherichia coli–mycobacteria shuttle vectors for operon and gene fusions to lacZ: the pJEM series. J Bacteriol 176:6749–6753
     [Google Scholar]
  29. Valdivia R. H., Hromockyj A. E., Monack D., Ramakrishnan L., Falkow S. 1996; Applications for the green fluorescent protein (GFP) in the study of host–pathogen interactions. Gene 173:47–52 [CrossRef]
     [Google Scholar]
  30. Yasuda S., Hirota Y. 1977; Cloning and mapping of the replication origin of Escherichia coli. Proc Natl Acad Sci USA 74:5458–5462 [CrossRef]
     [Google Scholar]
  31. Ye F., Renaudin J., Bové J.-M., Laigret F. 1994; Cloning and sequencing of the replication origin (oriC) of the Spiroplasma citri chromosome and construction of autonomously replicating artificial plasmids. Curr Microbiol 29:23–29 [CrossRef]
     [Google Scholar]
  32. Yee T. W., Smith D. W. 1990; Pseudomonas chromosomal replication origins: a bacterial class distinct from Escherichia coli-type origins. Proc Natl Acad Sci USA 87:1278–1282 [CrossRef]
     [Google Scholar]
  33. Zakrzewska-Czerwinska J., Schrempf H. 1992; Characterization of an autonomously replicating region from the Streptomyces lividians chromosome. J Bacteriol 174:2688–2693
     [Google Scholar]
  34. Zakrzewska-Czerwinska J., Nardmann J., Schrempf H. 1994; Inducible transcription of the dnaA gene from Streptomyces lividians 66. Mol Gen Genet 242:440–447 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-146-9-2199
Loading
Inhibition of chromosome replication in Mycobacterium smegmatis: effect of the rpmH–dnaA promoter region
Microbiology 146, 2199 (2000); https://doi.org/10.1099/00221287-146-9-2199
/content/journal/micro/10.1099/00221287-146-9-2199
/content/journal/micro/10.1099/00221287-146-9-2199
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error