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Volume 147, Issue 10

Differentiation of species by AFLP fingerprinting Free

Abstract

The fluorescent amplified fragment length polymorphism (AFLP) fingerprinting method was tested for its ability to identify and subtype the most important species found in veterinary infections. Sixty-nine reference strains and 19 clinical isolates of subsp. , subsp. , , , , subsp. , subsp. , subsp. , subsp. , , and were subjected to analysis. The topology of the dendrogram obtained by numerical analysis of the AFLP profiles did not reflect the phylogenetic relationships as derived from 16S rDNA sequence comparison. However, except for , AFLP analysis grouped the strains that belonged to the same genomic species into distinct clusters. strains were separated into two distinct AFLP groups, which corresponded with nalidixic-acid-sensitive and -resistant variants of . These results correlated with data from whole-cell protein profiling. Within , and , strains could be identified at the subspecies level. AFLP analysis also allowed the subtyping of most species at the strain level. It is concluded that AFLP analysis is a valuable tool for concurrent identification of campylobacters at the species, subspecies and strain levels. In addition, the data confirm and extend previous reports showing that is a heterogeneous species that may comprise multiple taxa.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): AFLP, amplified fragment length polymorphism , epidemiology , taxonomy , typing , UPGMA, unweighted pair-group method using arithmetic averages and veterinary infections
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2001-10-01
2024-04-30
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Differentiation of Campylobacter species by AFLP fingerprinting
Microbiology 147, 2729 (2001); https://doi.org/10.1099/00221287-147-10-2729
/content/journal/micro/10.1099/00221287-147-10-2729
/content/journal/micro/10.1099/00221287-147-10-2729
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