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Volume 148, Issue 5

Transglutaminase activity is involved in wall construction Free

Abstract

Transglutaminase activity, which forms the interpeptidic cross-link -(γ-glutamyl)-lysine, was demonstrated in cell-free extracts of by incorporation of [C]lysine into an exogenous acceptor, ,′-dimethylcasein. Higher levels of the activity were present in the cell wall, which also contained endogenous acceptors. The enzyme activity in the wall was inhibited by cystamine, a known inhibitor of transglutaminase, and by EDTA, indicating a cation-dependent activity. After the endogenous wall acceptors were labelled radioactively by transglutaminase, extraction with SDS solubilized about 50% of the total radioactivity, while Zymolyase and chitinase each released a further 3%. The proteins solubilized by SDS had molecular masses less than 50 kDa, whereas the material released by Zymolyase or chitinase had molecular masses greater than 180 kDa, suggesting a precursor–product relationship. Cystamine inhibited the growth of several strains of Treated cells showed increased sensitivity to Zymolyase and appeared as protoplasts, indicating gross alterations in the cell wall. These data suggest that transglutaminase may be involved in the formation of covalent cross-links between wall proteins during wall construction.

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Keyword(s): activity inhibition , cell wall , cross-linking , TGase, transglutaminase and yeast
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2002-05-01
2024-04-27
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References

  1. Aeschlimann D., Paulsson M. 1994; Transglutaminases: protein cross-linking enzymes in tissues and body fluids. Thromb Haemostasis 71:402–415
     [Google Scholar]
  2. Barsigian C., Fellin F. M., Jain A., Martinez J. 1988; Dissociation of fibrinogen and fibronectin binding from transglutaminase-mediated cross-linking at the hepatocyte surface. J Biol Chem 263:14015–14022
     [Google Scholar]
  3. Chandrashekar R., Mehta K. 2000; Transglutaminase-catalyzed reactions in the growth, maturation and development of parasitic nematodes. Parasitol Today 16:11–17 [CrossRef]
     [Google Scholar]
  4. Chowdhury Z. A., Barsigian C., Chalupowicz G. D., Bach T. L., Garcia-Manero G., Martinez J. 1997; Colocalization of tissue transglutaminase and stress fibers in human vascular smooth muscle cells and human umbilical vein endothelial cells. Exp Cell Res 231:38–49 [CrossRef]
     [Google Scholar]
  5. de Nobel J. G., Klis F. M., Priem J., Munnik T., van den Ende H. 1990; The glucanase-soluble mannoproteins limit cell wall porosity in Saccharomyces cerevisiae . Yeast 6:491–499 [CrossRef]
     [Google Scholar]
  6. Fonzi W. A. 1999; PHR1 and PHR2 of Candida albicans encode putative glycosidases required for proper cross-linking of beta-1,3- and beta-1,6-glucans. J Bacteriol 181:7070–7079
     [Google Scholar]
  7. Folk J. E. 1980; Transglutaminases. Annu Rev Biochem 49:517–531 [CrossRef]
     [Google Scholar]
  8. Ichinose A., Bottenus R. E., Davie E. W. 1990; Structure of transglutaminases. J Biol Chem 265:13411–13414
     [Google Scholar]
  9. Kapteyn J. C., van Den Ende H., Klis F. M. 1999; The contribution of cell wall proteins to the organization of the yeast cell wall. Biochim Biophys Acta 1426:373–383 [CrossRef]
     [Google Scholar]
  10. Kapteyn J. C., Hoyer L. L., Hecht J. E., Muller W. H., Andel A., Verkleij A. J., Makarow M., van den Ende H., Klis F. M. 2000; The cell wall architecture of Candida albicans wild-type cells and cell wall-defective mutants. Mol Microbiol 35:601–611
     [Google Scholar]
  11. Klis F. M. 1994; Cell wall assembly in yeast. Yeast 10:851–869 [CrossRef]
     [Google Scholar]
  12. Kollar R., Reinhold B. B., Petrakova E., Yeh H. J., Ashwell G., Drgonova J., Kapteyn J. C., Klis F. M., Cabib E. 1997; Architecture of the yeast cell wall β(1,6)-glucan interconnects mannoprotein, β(1,3)-glucan, and chitin. J Biol Chem 272:17762–17775 [CrossRef]
     [Google Scholar]
  13. Lorand L., Conrad S. M. 1984; Transglutaminases. Mol Cell Biochem 58:9–35 [CrossRef]
     [Google Scholar]
  14. Makarova K. S., Aravind L., Koonin E. V. 1999; A superfamily of archaeal, bacterial, and eukaryotic proteins homologous to animal transglutaminases. Protein Sci 8:1714–1719 [CrossRef]
     [Google Scholar]
  15. Martinez J., Rich E., Barsigian C. 1989; Transglutaminase-mediated cross-linking of fibrinogen by human umbilical vein endothelial cells. J Biol Chem 264:20502–20508
     [Google Scholar]
  16. Mrsa V., Seidl T., Gentzsch M., Tanner W. 1997; Protein specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O -mannosylated proteins of Saccharomyces cerevisiae . Yeast 13:1145–1154 [CrossRef]
     [Google Scholar]
  17. Popolo L., Vai M. 1999; The Gas1 glycoprotein, a putative wall polymer cross-linker. Biochim Biophys Acta 1426:385–400 [CrossRef]
     [Google Scholar]
  18. Ram A. F., Wolters A., Ten Hoopen R., Klis F. M. 1994; A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to calcofluor white. Yeast 10:1019–1030 [CrossRef]
     [Google Scholar]
  19. Ruiz-Herrera J., Iranzo M., Elorza M. V., Sentandreu R., Mormeneo S. 1995; Involvement of transglutaminase in the formation of covalent cross-links in the cell wall of Candida albicans . Arch Microbiol 164:186–193 [CrossRef]
     [Google Scholar]
  20. Serafini-Fracassini D., Del Duca S., Beninati S. 1995; Plant transglutaminases. Phytochemistry 40:355–365 [CrossRef]
     [Google Scholar]
  21. Simon M., Green H. 1985; Enzymatic cross-linking of involucrin and other proteins by keratinocyte particulates in vitro. Cell 40:677–683 [CrossRef]
     [Google Scholar]
  22. Smits G. J., Kapteyn J. C., van den Ende H., Klis F. M. 1999; Cell wall dynamics in yeast. Curr Opin Microbiol 2:348–352 [CrossRef]
     [Google Scholar]
  23. Staab J. F., Bradway S. D., Fidel P. L., Sundstrom P. 1999; Adhesive and mammalian transglutaminase substrate properties of Candida albicans Hwp1. Science 283:1535–1538 [CrossRef]
     [Google Scholar]
  24. Sundstrom P. 1999; Adhesins in Candida albicans . Curr Opin Microbiol 2:353–357 [CrossRef]
     [Google Scholar]
  25. Suzuki T., Park H., Hollingsworth N. M., Sternglanz R., Lennarz W. J. 2000; PNG1 , a yeast gene encoding a highly conserved peptide: N -glycanase. J Cell Biol 149:1039–1052 [CrossRef]
     [Google Scholar]
  26. Thacher S. M., Rice R. H. 1985; Keratinocyte-specific transglutaminase of cultured human epidermal cells: relation to cross-linked envelope formation and terminal differentiation. Cell 40:685–695 [CrossRef]
     [Google Scholar]
  27. van der Vaart J. M., Caro L. H., Chapman J. W., Klis F. M., Verrips C. T. 1995; Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae . J Bacteriol 177:3104–3110
     [Google Scholar]
  28. Vossen J. H., Muller W. H., Lipke P. N., Klis F. M. 1997; Restrictive glycosylphosphatidylinositol anchor synthesis in cwh6 / gpi3 yeast cells causes aberrant biogenesis of cell wall proteins. J Bacteriol 179:2202–2209
     [Google Scholar]
  29. Waffenschmidt S., Kusch T., Woessner J. P. 1999; A transglutaminase immunologically related to tissue transglutaminase catalyzes cross-linking of cell wall proteins in Chlamydomonas reinhardtii . Plant Physiol 121:1003–1015 [CrossRef]
     [Google Scholar]
  30. Zhu G., Spellman P. T., Volpe T., Brown P. O., Botstein D., Davis T. N., Futcher B. 2000; Two yeast forkhead genes regulate the cell cycle and pseudohyphal growth. Nature 406:90–94 [CrossRef]
     [Google Scholar]
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Transglutaminase activity is involved in Saccharomyces cerevisiae wall construction
Microbiology 148, 1329 (2002); https://doi.org/10.1099/00221287-148-5-1329
/content/journal/micro/10.1099/00221287-148-5-1329
/content/journal/micro/10.1099/00221287-148-5-1329
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