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Volume 148, Issue 9

Molecular characterization of an operon, , encoding a putative P1-type ATPase and a MerR-type regulatory protein involved in copper homeostasis in

The GenBank accession number for the sequence reported in this paper is AF390440.

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Abstract

The authors have characterized a chromosomally localized two-gene operon, , which encodes a putative P1-type ATPase, CueA, and a MerR-type metalloregulatory protein, CueR, in PNL-MK25. Disruption of by the insertion of mini-Tn:: into the wild-type strain led to a mutant strain with a sixfold reduction in its tolerance to copper; however, the tolerance of this mutant strain to the other seven related transition metals tested was not affected. The sensitivity of the mutant strain was attributed to a higher level of accumulation of intracellular copper, suggesting the involvement of CueA in copper export. Insertion of the cloned operon into the copper-sensitive mutant strain fully restored its tolerance to copper. :: expression studies confirmed that the operon was transcriptionally regulated by copper and CueR. Studies done on the mutant strain complemented with and revealed partial functional redundancy of and , respectively, in copper tolerance. Thus, the results of this study clearly suggest the involvement of in copper homeostasis in .

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Keyword(s): copper tolerance , CPx-type ATPase , GFP, green fluorescent protein , MIC, minimal inhibitory concentration and mini-Tn5::gfp mutagenesis
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2002-09-01
2024-04-27
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Molecular characterization of an operon, cueAR, encoding a putative P1-type ATPase and a MerR-type regulatory protein involved in copper homeostasis in Pseudomonas putidaThe GenBank accession number for the sequence reported in this paper is AF390440.
Microbiology 148, 2857 (2002); https://doi.org/10.1099/00221287-148-9-2857
/content/journal/micro/10.1099/00221287-148-9-2857
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