1887

Abstract

SUMMARY: The distribution of adapted particles of Vi-phage II has been examined. Fluctuation tests in which phage A was grown for a single cycle in type A of showed that phage E1 particles conformed to a Poisson distribution in a series of small samples and cannot, therefore, be spontaneous mutants of phage A. Particles of phage D1, on the other hand, showed a clonal distribution in a similar series of samples and are thus spontaneous host-range mutants of phage A. Phage E1 reverted to phage A during a single cycle of growth in type A organisms, which confirms that phage E1 is a phenotypic modification of phage A. In contrast, phage D1 was unaltered by a single cycle of propagation in type A organisms and its mutant nature is thus verified. Phage 29 has also been shown to be a host-range mutant of Vi-phage II. The adsorption of phage A to type E1 is lethal to the bacteria, of which only a small proportion support phage growth. The general applicability of these findings to the numerous adaptations of Vi-phage II is discussed.

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1956-08-01
2024-05-17
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References

  1. Adams M.H. 1950; Bacterial viruses. In Methods in Medical Research 2 Chicago: Year Book Publishers;
    [Google Scholar]
  2. Anderson E.S. 1951; The significance of Vi-phage types F1 and F2 of Salmonella typhi. . J. Hyg., Camb. 49:458
    [Google Scholar]
  3. Anderson E.S. 1955; Consideration of the Vi-phage types of Salmonella typhi on a structural basis. Nature; Lond.: 175171
    [Google Scholar]
  4. Anderson E.S. 1956; A new Vi-phage type of Salmonella typhi; with a discussion of methods of preparation of typing phages for new Vi-types. J. gen. Microbiol. 14:676
    [Google Scholar]
  5. Anderson E.S., Felix A. 1952; Variation in Vi-phage II of Salmonella typhi. . Nature; Lond.: 170492
    [Google Scholar]
  6. Anderson E.S., Felix A. 1953a; ‘Degraded Vi strains’ and variation in Vi- phage II of Salmonella typhi. . J. gen. Microbiol. 8:408
    [Google Scholar]
  7. Anderson E.S., Felix A. 1953b; The Vi type-determining phages carried by Salmonella typhi. . J. gen. Microbiol. 9:65
    [Google Scholar]
  8. Anderson E.S. 1953c; Vi-phage-type specificity and degradation in Salmonella typhi. . Proc. Vlth Congr. int. Microbiol.Rome 3:462
    [Google Scholar]
  9. Anderson E.S., Fraser A. 1955; The influence of the factors determining Vi- type specificity in Salmonella typhi on the adaptation of Vi-phage II. J. gen. Microbiol. 13:519
    [Google Scholar]
  10. Baron L.S., Formal S.B., Spilman W. 1955; Vi-phage-host interaction in Salmonella typhosa. . J. Bact. 69:177
    [Google Scholar]
  11. Bertani G. 1951; Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. . J. Bact. 62:293
    [Google Scholar]
  12. Bertani G., Weigle J.J. 1953; Host controlled variation in bacterial viruses. J. Bact. 65:113
    [Google Scholar]
  13. Craigie J., Yen C.H. 1938; The demonstration of types of B. typhosus by means of preparations of Type II Vi phage. Canad. publ. Hlth J. 29:448–484
    [Google Scholar]
  14. Delbrück M. 1940; The growth of bacteriophage and lysis of the host. J. gen. Physiol. 23:643
    [Google Scholar]
  15. Ellis E.L., Delbrück M. 1939; The growth of bacteriophage. J. gen. Physiol. 22:365
    [Google Scholar]
  16. Felix A. 1949; The nature of bacteriophages. Proc. IVth Congr. int. Microbiol., Copenhagen p. 355
    [Google Scholar]
  17. Felix A., Anderson E.S. 1951; Bacteriophages carried by the Vi-phage types of Salmonella typhi. . Nature; Lond.: 167603
    [Google Scholar]
  18. Luria S.E. 1951; The frequency distribution of spontaneous bacteriophage mutants as evidence for the exponential rate of phage reproduction. Cold Spr. Harb. Symp. quant. Biol. 16:463
    [Google Scholar]
  19. Luria S.E. 1953; Host-induced modifications of viruses. Cold Spr. Harb. Symp. quant. Biol. 18:237
    [Google Scholar]
  20. Luria S.E., Delbrück M. 1943; Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28:491
    [Google Scholar]
  21. Luria S.E., Human M.L. 1952; A non-hereditary, host-induced variation of bacterial viruses. J. Bact. 64:557
    [Google Scholar]
  22. Miles A.A., Misra S.S. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb. 38:732
    [Google Scholar]
  23. Weigle J.J., Bertani G. 1953; Variations des bactériophages conditionnées par les bactéries htes. Ann. Inst.Pasteur 84:175
    [Google Scholar]
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