1887

Abstract

SUMMARY: A number of proteins can be separated from purified preparations of tobacco mosaic virus; they differ from each other antigenically and all differ from the intact virus by not possessing all antigenic determinant groups possessed by the virus. Some of these proteins are easily detached from the virus by such mild treatments as placing it in a protein solution or in agar gel. These are antigenically identical with the ‘ -protein ’ which remains in the supernatant fluid when the virus is sedimented by ultracentrifugation from sap of infected plants. More of these proteins are detached from the virus by placing it in borate buffer at pH 8·7. When a more drastic treatment is applied, such as incubation at . pH 10, which disintegrates a proportion of the virus, still more of these proteins are released, but then some proteins antigenically different from those of ‘ -protein ’ are also released.

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/content/journal/micro/10.1099/00221287-16-2-405
1957-04-01
2024-05-04
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References

  1. Bawden F. C., Pirie N. W. 1956; Observations on the anomalous proteins occurring in extracts from plants infected with strains of tobacco mosaic virus. J. gen. Microbiol 14:460
    [Google Scholar]
  2. Commoner B., Rodenberg S. D. 1955; Relationships between tobacco mosaic virus and the non-virus proteins. J. gen. Physiol 38:475
    [Google Scholar]
  3. Commoner B., Yamada M., Rodenberg S. D., Wang T., Basler E. 1953; The proteins synthesized in tissue infected with tobacco mosaic virus. Science 118:529
    [Google Scholar]
  4. Franklin R. E., Holmes K. C. 1956; The helical arrangement of the protein subunits in tobacco mosaic virus. Biochim. biophys. Acta 21:405
    [Google Scholar]
  5. Jeener R., Lemoine P., Lavand’Homme C. 1954; Détection et propriétés de formes du virus de la mosaique du tabac dépourvue d’acide ribonucléique et non infectieuses. Biochim. biophys. Acta 14:321
    [Google Scholar]
  6. Oakley C. L., Fulthorpe A. J. 1953; Antigenic analysis by diffusion. J. Path. Bact 65:49
    [Google Scholar]
  7. Ouchterlony O. 1949; Antigen-antibody reactions in gels. Ark. Kemi Min. Geol 26B:14
    [Google Scholar]
  8. Sang J. H., Sobey W. R. 1954; The genetic control of response to antigenic stimuli. J. Immunol 72:52
    [Google Scholar]
  9. Schramm G. 1943; Über die Spaltung des Tabakmosaikvirus in niedermolekulare Proteine und die Rückbildung hochmolekularen Proteins aus den Spaltstücken. Naturwissenschaften 31:94
    [Google Scholar]
  10. Schramm G., Schumacher G., Zillig W. 1955; Über die Struktur des Tabakmosaikvirus. III. Mitt.: Der Zerfall in alkalischer Lösung. Z. Naturf 10b:481
    [Google Scholar]
  11. Schramm G., Zillig W. 1955; Über die Struktur des Tabakmosaikvirus. IV. Mitt.; Die Reaggregation des nucleinsäurefreien Proteins. Z. Naturf 10b:493
    [Google Scholar]
  12. Slogteren D. H. M. van. 1955; Gel diffusion of tobacco mosaic virus, demonstrated by serological analysis of its components and by electron-microscopy. Acta bot. Neerl 4:472
    [Google Scholar]
  13. Starlinger P. 1955; Vergleich der serologischen Spezifität des Tabakmosaikvirus mit nucleinsäure-freien und -haltigen Abbauprodukten des Virus. Z. Naturf 10b:339
    [Google Scholar]
  14. Takahashi W. N., Ishii M. 1952; An abnormal protein associated with tobacco mosaic virus infection. Nature; Lond: 169419
    [Google Scholar]
  15. Takahashi W. N., Ishii M. 1953; A macromolecular protein associated with tobacco mosaic virus infection: its isolation and properties. Amer. J. Bot 40:85
    [Google Scholar]
  16. Wilson M. W., Pringle B. H. 1954; Experimental studies of the agar-plate precipitin test of Ouchterlony. J. Immunol 73:232
    [Google Scholar]
  17. Wilson M. W., Pringle B. H. 1955; Interpretation of the Ouchterlony precipitin test. J. Immunol 75:460
    [Google Scholar]
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