1887

Abstract

SUMMARY: A purified cellular polysaccharide was obtained from a non-capsulated pneumococcus ( strain R19, derived from a Type II organism). The main purification steps were peptic, tryptic and ribonuclease digestions, removal of protein by the Sevag technique and ethanol precipitation of the polysaccharide. Ribonuclease digestion was an essential step in the purification procedure. The constituent sugars of the isolated polysaccharide were galactose, mannose and glucosamine. The acetyl content of the polysaccharide indicated that the glucosamine was fully acetylated on the N position and that some O-acetyl was also present. The phosphorus content was not entirely explicable in terms of the known ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) contamination. Residual amino acid was consistently present which may have been due to bound amino acids or to protein contamination.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-17-2-437
1957-10-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/17/2/mic-17-2-437.html?itemId=/content/journal/micro/10.1099/00221287-17-2-437&mimeType=html&fmt=ahah

References

  1. Ceriotti G. 1955; Determination of nucleic acids in animal tissues. J. biol. Chem 214:59
    [Google Scholar]
  2. Consden R., Stanier W. M. 1952; Ionophoresis of sugars on paper and some applications to the analysis of protein-polysaccharide complexes. Nature; Land: 169783
    [Google Scholar]
  3. Conway E. J. 1947 Micro-Diffusion Analysis and Volumetric Error, 2nd ed. London: Crosby-Lockwood;
    [Google Scholar]
  4. Enders J. F. 1930; A type specific substance distinct from the specific carbohydrate in pneumococcus Type I. J. exp. Med 52:235
    [Google Scholar]
  5. Griswold B. L., Humoller F. L., McIntyre A. R. 1951; Inorganic phosphates and phosphate esters in tissue extracts. Analyt. Chem 23:192
    [Google Scholar]
  6. Hagedorn H. C., Jensen B. N. 1923; Zur Mikrobestimmung des Blutzuckers mittels Ferricyanid. Biochem. Z 135:46
    [Google Scholar]
  7. Heidelberger M., Kendall F. E. 1931; Specific and non-specific polysaccharides of Type IV pneumococcus. J. exp. Med 53:625
    [Google Scholar]
  8. Heidelberger M., Kendall F. E., Scherp H. W. 1936; The specific polysaccharides of Types I, II and III pneumococcus. A review of methods and data. J. exp. Med 64:559
    [Google Scholar]
  9. Ma T. S., Zuazaga G. 1942; Micro-Kjeldahl determination of nitrogen. A new indicator and an improved rapid method. Industr. Engng Chem. (Anal.) 14:280
    [Google Scholar]
  10. Partridge S. M. 1948; Filter paper partition chromatography of sugars. Biochem. J 42:238
    [Google Scholar]
  11. Partridge S. M. 1949; Aniline hydrogen phthalate as a spraying reagent for chromatography of sugars. Nature; Lond: 164443
    [Google Scholar]
  12. Paul J. 1956; The chemical determination of deoxyribonucleic acid in tissue cultures. J. Biophysic. Biochem. Cytol 2:797
    [Google Scholar]
  13. Rondle C. J. M., Morgan W. T. J. 1955; The determination of glucosamine and galactosamine. Biochem. J 61:586
    [Google Scholar]
  14. Sabin A. B. 1931; On the presence in antipneumococcus serum of type-specific protective antibody not neutralized by homologous specific soluble substance. J. exp. Med 53:93
    [Google Scholar]
  15. Sevag M. G. 1934; Eine neue physikalischeEnteiweisungsmethodezurDarstellung biologisch wirksamer Substanzen. Biochem. Z 273:419
    [Google Scholar]
  16. Smith E. E. B., Mills G. T., Harper E. M. 1957a; A comparison of the uridine pyrophosphoglycosyl metabolism of capsulated and non-capsulated pneumococci. J. gen. Microbiol 16:426
    [Google Scholar]
  17. Smith E. E. B., Mills G. T., Harper E. M. 1957b; The isolation of uridine pyrophosphogalacturonic acid from a Type I pneumococcus. Biochim. biophys. Acta 23:662
    [Google Scholar]
  18. Smith H., Zwartouw H. T. 1956; The polysaccharide from Bacillus anthracis grown in vivo. Biochem. J 63:447
    [Google Scholar]
  19. Tillett W. S., Francis T. Jun 1930; Serological reactions in pneumonia with a non-protein somatic fraction of pneumococcus. J. exp. Med 52:561
    [Google Scholar]
  20. Tillett W. S., Goebel W. F., Avery O. T. 1930; Chemical and immunological properties of a species-specific carbohydrate of pneumococci. J. exp. Med 52:895
    [Google Scholar]
  21. Wadsworth A., Brown R. 1931; Chemical and immunological studies of the pneumococcus. I. A specific antigenic carbohydrate of Type I pneumococcus. J. Immunol 21:245
    [Google Scholar]
  22. Wadsworth A., Brown R. 1933; Chemical and immunological studies of the pneumococcus. III. Cellular carbohydrate fractions. J. Immunol 24:349
    [Google Scholar]
  23. Wadsworth A., Crowe M. O’L., Smith L. A. 1934; Absorption spectra of the carbohydrates of the pneumococcus. A preliminary note. J. Immunol 26:481
    [Google Scholar]
  24. Ward H. K. 1932; An examination of the mechanism of pneumococcus immunity by means of bactericidal measurements. II. The reaction between the anticarbohydrate antibody and type-specific products of the organism. J. exp. Med 55:519
    [Google Scholar]
  25. White B. 1938 Biology of the Pneumococcus New York: Commonwealth Fund;
    [Google Scholar]
  26. Wyatt G. R. 1951; Recognition and estimation of 5-methyl cytosine in nucleic acids. Biochem. J 48:581
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-17-2-437
Loading
/content/journal/micro/10.1099/00221287-17-2-437
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error