1887

Abstract

SUMMARY: Parallel physiological and cytological investigations of a chlortetra-cycline-producing culture—, strain 536—indicate some intracellular causes of the dependence of chlortetracycline biosynthesis upon the inorganic phosphorus content of the nutrient medium.

Increase of inorganic phosphorus in the fermentation medium led to a formation of large nuclear elements containing much DNA (in corn steep medium) or ribonucleo-proteid complexes in the shape of large volutin granules (corn steep liquor ash medium). As compared with the control, the nuclear substance/cytoplasm ratio in the hyphae is increased. The ability of mycelium overloaded with nuclear elements or volutin granules for chlortetracycline biosynthesis is markedly decreased. The iufluence of phosphorus on the microscopical structure of mycelium and biosynthesis of antibiotic depends on the composition of medium. The poor quality of certain batches of corn steep liquor used for the production of chlortetracycline is largely due to their high content of inorganic phosphorus.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-20-3-462
1959-06-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/20/3/mic-20-3-462.html?itemId=/content/journal/micro/10.1099/00221287-20-3-462&mimeType=html&fmt=ahah

References

  1. Belozersky A. N. 1945; On the chemical nature of volutin. Microbiology, Moscow 14:129
    [Google Scholar]
  2. Berill N., Huskins C. 1936; The ‘resting’ nucleus. Amer. Nat 70:257
    [Google Scholar]
  3. Biffi G., Boretti G., Di Marco A., Penella P. 1954; Metabolic behaviour and chlortetracyclin production by Streptomyces aureofaciens in liquid culture. Appl. Microbiol 2:288
    [Google Scholar]
  4. Boretti G., Di Marco A., Scotti T., Zocchi P. 1955; Variazioni morphologiche e biochimiche dello Streptomyces aureofaciens in relazione alia produzione di chlorotetracyclina. G. Microbiol 1:97
    [Google Scholar]
  5. Brinberg S. L., Grabovskaya O. B. 1958; The significance of phosphorus for the biosynthesis of streptomycin. Microbiology, Moscow 27:407
    [Google Scholar]
  6. Bringmann G. 1951; Elektromikroskopische und Lichtmikroskopische Studien zur Morphologie und Entwicklung von Streptomyces griseus Krainsky. Zbl. Bakt 157:349
    [Google Scholar]
  7. Carvajal F. 1947; The production of spores in submerged cultures by some Streptomyces . Mycologia 39:425
    [Google Scholar]
  8. Demianovskaya N. S., Belozersky A. N. 1954; Desoxyribonucleic acid of Actinomyces globisporus streptomycini Kras, in the process of its development. Biochem. Leningrad 19:688
    [Google Scholar]
  9. Guberniev M. A., Ugoleva N. A., Torbochkina L. M. 1956; Nucleic acids and phosphorus compounds in the mycelium of Actinomyces aureofaciens at different stages of development. Antibiotiki, Moscow 3:8
    [Google Scholar]
  10. Hagedorn H. 1955a; Untersuchungen über den isoelektrischen Punkt bei Actinomyceten. Protoplasma 45:1115
    [Google Scholar]
  11. Hagedorn H. 1955b; Beiträge zur Cytologie und Morphologie der Actinomyceten. Z. Bakt II. Abt 108:353
    [Google Scholar]
  12. Kelley E. 1939; Reactions of dyes with cell substances. J. biol. Chem 127:55
    [Google Scholar]
  13. Marco A. Di. 1956; Metabolism of Streptomyces aureofaciens and biosynthesis of chlortetracyclin. G. microbiol 2:285
    [Google Scholar]
  14. Peshkov M. A., Rautenstein J. I., Sorokina M. M., Cherednichenko A. F., Sharkova A. S. 1952; Cytological changes of the Actinomyces globisporus streptomycini mycelium under the influence of actinophage. Microbiology, Moscow 21:665
    [Google Scholar]
  15. Peter K. 1940; Die indirekte Teilung der Zelle in ihren Beziehungen zu Tätigkeit, Differenzierung und Wachstum. Z. Zellforsch 30:721
    [Google Scholar]
  16. Prokofieva-Belgovskaya A. A., Demianovskaya N. S. 1957; Cytological and biochemical changes in Actinomyces globisporus streptomycini Kras, in the process of antibiotic production in submerged culture. Microbiology, Moscow 26:22
    [Google Scholar]
  17. Prokofieva-Belgovskaya A. A., Kapitonova O. N. 1959; Isoelectric point of cytoplasm and nuclear elements of Actinomyces streptomycini Kras. Bull. Acad. Sci., U.S.S.R in the Press
    [Google Scholar]
  18. Prokofieva-Belgovskaya A. A., Orlova N. V. 1956; The peculiarities of growth and development of actinomycetes producing streptomycin, biomycin, and terramycin in submerged cultures. Bull. Acad. Sci., U.S.S.R 5:59
    [Google Scholar]
  19. Prokofieva-Belgovskaya A. A., Pestereva G. D., Rudaya S. M. 1956; Peculiarities of growth and development of Actinomyces rimosus under the conditions of bottom fermentation. Microbiology, Moscow 25:668
    [Google Scholar]
  20. Prokofieva-Belgovskaya A. A., Popova L. A., Orlova N. V., Severin V. A., Gorskaya S. Y., Kapitonova O. N. 1957; Dependence of the antibiotic activity of actinomycetes on the peculiarities of their growth and development. Abstracts of reports presented at the Second All-Union Conference on Antibiotics Russian 52:
    [Google Scholar]
  21. Scotti T., Zocchi P. 1955; Studio della struttura del micelio di Streptomyces aureofaciens. G. microbiol 1:35
    [Google Scholar]
  22. Shen S. C., Soohg H. Y., Chen J. P., Hung M. M., Yin H. C. 1956; III. The effect of phosphate on the utilization of carbohydrates by Streptomyces aureofaciens and the production of aureomycin. Acta Exp. Biol. Sinica 5:2
    [Google Scholar]
  23. Vendrely R., Tulasne R., Minck R. 1948; Premières observations sur les modifications chimiques qu’apporte la penicilline chez le Staphylocoque. C.R. Soc. Biol., Paris 142:239
    [Google Scholar]
  24. Wiame J. 1954; Presence of a polyphosphoric acid nucleic derivative in yeast. C.R. Soc. Biol., Paris 140:897
    [Google Scholar]
  25. Zalkind S. J. 1952; Mitosis and the functional activity of the cell. Advanc. Modern Biol 33:431 Russian
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-20-3-462
Loading
/content/journal/micro/10.1099/00221287-20-3-462
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error