1887

Abstract

SUMMARY: The type and subtype specificities of the interactions between fractions of the virus system of foot-and-mouth disease and its antibodies were investigated by complement-fixation methods. Specificity is discussed in terms of a cross-fixation ratio. The 25 m infective component (D-fraction) combines homotypically with antibody. Thermal degradation of the 25 m component produces a smaller component of enhanced activity which combines heterotypically with antibody and resembles the naturally occurring 7 m component (U-fraction). The influence of heating upon the 7 m component is marked by a sharpened specificity. The specificity and activity of unfractionated starting materials may be interpreted in terms of those of the 25 and 7 m components present in the separable fractions. Significant differences between the reactions observed in the long and short incubation procedures emphasize the advantages of the latter in specificity studies. The composition and treatment of the initial antigens and the test procedures employed must be carefully defined in such studies of antigen-antibody combination.

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/content/journal/micro/10.1099/00221287-22-2-405
1960-04-01
2024-04-27
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References

  1. Bradish C. J., Brooksby J. B., Dillon J. F., Norambuena M. 1952; Ultracentrifugal studies on the infective and complement-fixing components in the virus system of foot-and-mouth disease. Proc. roy. Soc. B 140:107
    [Google Scholar]
  2. Bradish C. J., Brooksby J. B., Dillon J. F. Jun 1956; Biophysical studies of the virus system of vesicular stomatitis. J. gen. Microbiol 14:290
    [Google Scholar]
  3. Bradish C. J., Henderson W. M., Kirkham J. B. 1960; Concentration and electron microscopy of the characteristic particle of foot-and-mouth disease. J. gen. Microbiol 22:379
    [Google Scholar]
  4. Bradish C. J., Brooksby J. B., Tsubahara H. 1960; The complement-fixation test in studies of the components of the virus system of foot-and-mouth disease and its antibodies. J. gen. Microbiol 22:392
    [Google Scholar]
  5. Brooksby J. B. 1952; The technique of complement-fixation in foot-and-mouth disease research. A.R.C. Rep. Ser12
    [Google Scholar]
  6. Brooksby J. B. 1953 In The Nature of Virus Multiplication, Symp. Soc. gen. Microbiol 2:246
    [Google Scholar]
  7. Brooksby J. B. 1958; The virus of foot-and-mouth disease. Advanc. Virus Res 5:1
    [Google Scholar]
  8. Brown F., Crick J. 1958; Application of agar gel precipitin tests to the study of the virus of foot-and-mouth disease. Virology 5:133
    [Google Scholar]
  9. Galloway I. A., Henderson W. M., Brooksby J. B. 1948; Strains of the virus of foot-and-mouth disease recovered from outbreaks in Mexico. Proc. Soc. exp. Biol., N.Y 69:57
    [Google Scholar]
  10. Skinner H. H., Henderson W. M., Brooksby J. B. 1952; Use of unweaned white mice in foot-and-mouth disease research. Nature; Lond: 169794
    [Google Scholar]
  11. Traub E., Möhlmann H. 1946; Untersuchungen über immunologische Varianten der Typen A und B des Maul-und-Klauenseuchevirus. Berl. Münch. tierärztl. Wschr 1:1
    [Google Scholar]
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