1887

Abstract

SUMMARY: The uptake of iron by growing in complex medium was found to be rapid and quantitative, even when the iron concentration of the medium was high enough to abolish toxin production completely. The presence of this inhibitory concentration of iron (3 g./ml.) did not alter the duration of logarithmic growth nor did it affect the shape of the growth curve in any other way. The ferrous and ferric forms of iron were taken up equally well by the organism and appeared, weight for weight, to give the same final toxin titres. Ferrous iron, however, appeared to begin to exert its effect on toxin synthesis much earlier in the growth period than did ferric iron. It is suggested that perhaps only ferrous iron is inhibitory and that ferric iron requires to be converted to the ferrous form before exerting its effect.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-22-3-705
1960-06-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/22/3/mic-22-3-705.html?itemId=/content/journal/micro/10.1099/00221287-22-3-705&mimeType=html&fmt=ahah

References

  1. Clarke G. D. 1958; The effect of ferrous irons on the formation of toxin and porphyrin by a strain of Corynebacterium diphtheriae . J. gen. Microbiol 18:698
    [Google Scholar]
  2. Edwards D. C. 1960; The growth and toxin production of Corynebacterium diphtheriae in submerged culture. J. gen. Microbiol 22:699
    [Google Scholar]
  3. Glenny A. T., Okell C. C. 1924; The titration of diphtheria toxin and antitoxin by flocculation methods. J. Path. Bact 27:187
    [Google Scholar]
  4. Locke A., Main E. R. 1931; The relation of copper and iron to production of toxin and enzyme action. J. infect. Dis 48:419
    [Google Scholar]
  5. Pappenheimer A. M. Jun. 1947; Diphtheria toxin. III. A reinvestigation of the effect of iron on toxin and porphyrin production. J. biol. Chem 167:251
    [Google Scholar]
  6. Pappenheimer A. M. Jun. 1955; The pathogenesis of diphtheria. Symp. Soc. gen. Microbiol 5:40
    [Google Scholar]
  7. Pappenheimer A. M. Jun. Johnson S. J. 1936; Studies in diphtheria toxin production. I. The effect of iron and copper. Brit. J. exp. Path 17:335
    [Google Scholar]
  8. Pope C. G. 1932; The production of toxin by C. diphtheriae. II. Effects produced by addition of iron and copper to the medium. Brit. J. exp. Path 13:218
    [Google Scholar]
  9. Ramon G. 1922; Floculation dans un mélange neutre de toxin-antitoxine diphtériques. C.R. Soc. Biol., Paris 86:661
    [Google Scholar]
  10. Raynaud M., Alouf J., Mangalo R. 1959; Croissance et toxinogénèse diphtériques en culture agitée, sur milieu synthétique. Ann. Inst. Pasteur 96:276
    [Google Scholar]
  11. Seamer P. A. 1959; Estimation of microgram quantities of iron in culture medium using bathophenanthroline. Nature; Lond: 184636
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-22-3-705
Loading
/content/journal/micro/10.1099/00221287-22-3-705
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error