1887

Abstract

Summary: The microscopic elements which constituted cultures of a stable L form (Proteus L9) were found to be highly heterogeneous with respect to morphological, chemical and metabolic properties. The smallest elements found in the cultures studied (diameter < 0·3 ) contained more lipid-phosphorus (lipid-P), but less ribonucleic acid-phosphorus, deoxyribonucleic acid-phosphorus and protein-nitrogen, than whole cultures consisting predominantly of bodies of diameter > 1 . The small elements respired at about the same rate as whole cultures, but they showed low, if any, biosynthetic activity. The small elements probably possess a structural organization similar to that of L bodies of larger sizes, but most of them contained little, if any DNA.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-24-3-379
1961-03-01
2024-05-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/24/3/mic-24-3-379.html?itemId=/content/journal/micro/10.1099/00221287-24-3-379&mimeType=html&fmt=ahah

References

  1. Abrams R. Y. 1955; A method for the cultivation of L forms in liquid media. J. Bact 70:251
    [Google Scholar]
  2. Alexander M., Wilson P. W. 1955; Enzyme localization in Azotobader vinelandii . Proc. nat. Acad. Sci., Wash 41:843
    [Google Scholar]
  3. Allen R. J. L. 1940; The estimation of phosphorus. Biochem. J 34:858
    [Google Scholar]
  4. Backus R. C., Williams R. C. 1950; The use of spraying methods and of volatile suspending media in the preparation of specimens for electron microscopy. J. appl. Phys 21:11
    [Google Scholar]
  5. Bonnichsen R. K., Chance B., Theorell H. 1947; Catalase activity. Acta chem. scand 1:685
    [Google Scholar]
  6. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J 62:315
    [Google Scholar]
  7. Dienes L., Weinberger H. J. 1951; The L forms of bacteria. Bact. Rev 15:245
    [Google Scholar]
  8. Few A. V., Fraser M. J., Gilby A. R. 1957; The intracellular catalase of Micrococcus lysodeikticus . Biochim. biophys. Acta 24:306
    [Google Scholar]
  9. Gilby A. R., Few A. V., McQuillen K. 1958; The chemical composition of the protoplast membrane of Micrococcus lysodeikticus . Biochim. biophys. Acta 29:21
    [Google Scholar]
  10. Kandler O., Kandler G. 1960; Die L-Phase der Bakterien. Ergebn. Mikrobiol 33:97
    [Google Scholar]
  11. Klieneberger-Nobel E. 1956; Über die Wesensverschiedenheit der peripneumonie-ähnlichenOrganismen und der L-Phase der Bakterien. Zbl. Bakt 1 Abt 167:345
    [Google Scholar]
  12. Klieneberger-Nobel E. 1960; L-forms of bacteria. In The bacteria Gunsalus I. C., Stanier R. Y. ed I p. 443 New York and London: Academic Press Inc;
    [Google Scholar]
  13. Lindberg O., Ernster L. 1956; Determination of organic phosphorus compounds by phosphate analysis. In Methods of biochemical analysis Glick D. ed III p. 1 New York and London: Interscience Publishers;
    [Google Scholar]
  14. Loring H. S. 1955; Hydrolysis of nucleic acids and procedures for the direct estimation of purine and pyrimidine fractions by absorption spectrophotometry. In The Nucleic Acids Chargaff E., Davidson J. N. ed I p. 91 New York: Academic Press Inc;
    [Google Scholar]
  15. Mandel P., Terranova T., Sensenbrenner M. 1957; Fractionnement des formes L fixées du Proteus P 18. C.R. Acad. Sci., Paris 245:1469
    [Google Scholar]
  16. Mandel P., Feo F., Sensenbrenner M., Terranova T. 1959; Étude de quelques activités enzymatiques de divers types morphologiques de formes L stables dérivées du Proteus P 18. C.R. Acad Sci., Paris 248:315
    [Google Scholar]
  17. Mandel P., Terranova T., Sensenbrenner M., Feo F. 1959; Oxygen consumption of different forms of stable L Proteus P 18 growing in hypertonic liquid medium. Nature; Lond: 183194
    [Google Scholar]
  18. Mandel P., Sensenbrenner M., Terranova T., Gregario G., Bader A. M. 1959; Distribution of nucleic acids among different stable L forms of Proteus P. 18. Nature; Lond: 184566
    [Google Scholar]
  19. Marr A. G. 1960; Localization of enzymes in bacteria. In The Bacteria Gunsalus I. C., Stanier R. Y. ed I p. 443 New York and London: Academic Press Inc;
    [Google Scholar]
  20. Marr A. G., Cota-Robles E. H. 1957; Sonic disruption of Azotobacter vinelandii . J. Bact 74:79
    [Google Scholar]
  21. Mickle H. 1948; Tissue disintegrator. J. R. Micr. Soc 68:10
    [Google Scholar]
  22. Mitchell P., Moyle J. 1951; The glycerophospho-protein complex envelope of Micrococcus pyogenes . J. gen. Microbiol 5:981
    [Google Scholar]
  23. Mitchell P., Moyle J. 1956; The cytochrome system in the plasma membrane of Staphylococcus aureus . Biochem. J 64:19P
    [Google Scholar]
  24. Schneider W. C. 1945; Phosphorus compounds in animal tissues. I. Extraction and estimation of desoxypentose nucleic acid and of pentosenucleic acid. J. biol. Chem 161:293
    [Google Scholar]
  25. Storck R., Wachsman J. 1957; Enzyme localization in Bacillus megaterium . J. Bact 73:784
    [Google Scholar]
  26. Taubeneck U. 1956; Die Penicillininaktivierung durch Proteus-Arten. Zbl. Bakt 1. Abt 167:345
    [Google Scholar]
  27. Thorsson K. G., Weibull C. 1958; Studies on the structure of bacterial L forms, protoplasts and protoplast-like bodies. J. Ultrastructure Res 1:412
    [Google Scholar]
  28. Tulasne R. 1955; Bilan de nos connaissances sur les cycles bactériens du type L etsur les formes L des bactéries. Biol, méd., Paris 44:391
    [Google Scholar]
  29. Weibull C. 1957; The lipids of a lysozyme sensitive Bacillus species (Bacillus ‘M’). Acta chem. scand 11:881
    [Google Scholar]
  30. Weibull C. 1960; Counting procedure for phase contrast microscopy. J. Bact 79:155
    [Google Scholar]
  31. Weibull C., Beckman H. 1960; Growth of bacterial L forms and bacterial protoplasts. J. Bact 79:638
    [Google Scholar]
  32. Weibull C., Beckman H., Bergström L. 1959; Localization of enzymes in Bacillus megaterium, strain M. J. gen. Microbiol 20:519
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-24-3-379
Loading
/content/journal/micro/10.1099/00221287-24-3-379
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error