1887

Abstract

Summary: Costilow & Humphreys’s (1955) observation that certain strains of reduced nitrates under certain conditions was confirmed. Two strains of also reduced nitrates. In static culture, agar and anaerobiosis were not essential for nitrate reduction, contrary to speculations in the literature. Nitrate reduction was possible only in media with restricted carbohydrate and with the pH value maintained at a relatively high value within the activity range of nitrate reductases. For good growth, lactobacilli for the nitrate test have been customarily grown in media with high carbohydrate content, with consequent low final pH values. This seems to be the essential reason why the genus had previously been defined as unexceptionally nitratase-negative.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-24-3-401
1961-03-01
2024-05-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/24/3/mic-24-3-401.html?itemId=/content/journal/micro/10.1099/00221287-24-3-401&mimeType=html&fmt=ahah

References

  1. Bergey’s Manual of Determinative Bacteriology 1948, 6th ed. Breed R. S., Murray E. G. D., Hitchins A. P. Ed Baltimore: Williams and Wilkins;
  2. Clarke R. T. J. 1959; A dextran-fermenting organism from the rumen closely resembling Lactobacillus bifidus . J. gen. Microbiol 20:549
    [Google Scholar]
  3. Costilow R. N., Humphreys T. W. 1955; Nitrate reduction by certain strains of Lactobacillus plantarum . Science 121:168
    [Google Scholar]
  4. Frazier W. C., Whittier E. O. 1931; Studies on the influence of bacteria on the oxidation-reduction potential of milk. I. Influence of pure cultures of milk organisms. J. Bact 21:239
    [Google Scholar]
  5. Fred E. B., Peterson W. H., Anderson J. A. 1921; The characteristics of certain pentose-destroying bacteria, especially as concerns their action on arabinose and xylose. J. biol. Chem 48:385
    [Google Scholar]
  6. Man J. C. de, Rogosa M., Sharpe M. E. 1960; A medium for cultivation of lactobacilli. J. appl. Bact 23:130
    [Google Scholar]
  7. Najjar V. A. 1955; Nitrite metabolism. Enzymatic formation of nitrogen gas (N2) from nitrite and nitric oxide gas (NO). Formation of nitric oxide gas from nitrite. In Methods of Enzymology 2420 Colowick S. P., Kaplan N. O. Ed New York: Academic Press Inc;
    [Google Scholar]
  8. Nason A., Evans H. J. 1955; Nitrate reductase from Neurospora . In Methods in Enzymology 2411 Colowick S. P., Kaplan N. O. Ed New York: Academic Press Inc;
    [Google Scholar]
  9. Orla-Jensen S., Orla-Jensen A. D., Winther O. 1936; Bacterium bifidum und Thermobacterium intestinale . Zbl. Bakt II. Abt 93:321
    [Google Scholar]
  10. Rogosa M., Wiseman R. F., Mitchell J. A., Disraely M., Bearman A. J. 1953; Species differentiation of oral lactobacilli from man including descriptions of Lactobacillus salivarius nov.spec. and Lactobacillus cellobiosus nov.spec. J. Bact 65:681
    [Google Scholar]
  11. Rogosa M., Sharpe M. E. 1959; An approach to the classification of the lactobacilli. J. appl. Bact 22:329
    [Google Scholar]
  12. Sacks L. E., Barker H. A. 1949; The influence of oxygen on nitrate and nitrite reduction. J. Bact 58:11
    [Google Scholar]
  13. Sharpe M. E. 1955; Serological classification of lactobacilli. J. gen. Microbiol 12:107
    [Google Scholar]
  14. Sundman V., Björkstein K., Gyllenberg H. G. 1959; Morphology of the bifid bacteria (organisms previously incorrectly designated Lactobacillus bifidus) and some related genera. J. gen. Microbiol 21:371
    [Google Scholar]
  15. Tittsler R. P. 1930; The reduction of nitrates to nitrites by Salmonella pullorum and Salmonella gallinarum . J. Bact 19:261
    [Google Scholar]
  16. Wallace G. I., Neave S. L. 1927; The nitrite test as applied to bacterial cultures. J. Bact 14:377
    [Google Scholar]
  17. Woods D. D. 1938; The reduction of nitrate to ammonia by Clostridium welchii . Biochem. J 32:2000
    [Google Scholar]
  18. ZoBell C. E. 1932; Factors influencing the reduction of nitrates and nitrites by bacteria in semisolid media. J. Bact 24:273
    [Google Scholar]
  19. Zucker M., Nason A. 1955; Hydroxylamine reductase from Neurospora crassa. In Methods in Enzymology 2416 Colowick S. P., Kaplan N. O. Ed New York: Academic Press Inc;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-24-3-401
Loading
/content/journal/micro/10.1099/00221287-24-3-401
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error