1887

Abstract

SUMMARY: A mutant of was isolated which showed a requirement for methionine+lysine when a fairly large inoculum of washed organisms was used. Cystathionine or homocysteine replaced methionine, and diaminopimelic acid replaced lysine; no other amino acid or growth factor was active. The amounts of the amino acids required to promote growth were only about one-tenth of the quantity of methionine and lysine found in the grown organisms. A quantitative study with C-glucose and S-sulphate confirmed that these amino acids were synthesized . Although homoserine would not replace methionine for growth, the presence of unlabelled homoserine suppressed the incorporation of isotope from C-glucose into methionine and threonine, indicating that the accepted pathways are operative and that the block in methionine synthesis was in the formation of cystathionine. Small amounts of yeast extract replaced the amino acid requirement and decreased one-thousandfold the size of the inoculum required.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-27-1-41
1962-01-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/27/1/mic-27-1-41.html?itemId=/content/journal/micro/10.1099/00221287-27-1-41&mimeType=html&fmt=ahah

References

  1. Abelson P. H., Bolton E., Britten R., Cowie D. B., Roberts R. V. 1958; Synthesis of the aspartic and glutamic families of amino acids in Escherichia coli . Proc. not. Acad. Set., Wash 39:1020
    [Google Scholar]
  2. Abelson P. H. 1954; Amino add biosynthesis in Escherichia coli: Isotopic competition with 14C-glucose. J. biol. Chem 206:335
    [Google Scholar]
  3. Adelberg E. A., Meyers J. W. 1953; Modification of the penicillin technique for the selection of auxotrophic bacteria. J. Bad 65:848
    [Google Scholar]
  4. Anderson R. F., Rhodes R. A., Nelson G. E. N., Shekleton M. C., Barreto A., Arnold M. 1958; Lysine, methionine and tryptophan content of microorganisms. I. Bacteria. J. Bad 76:131
    [Google Scholar]
  5. Connell G. E., Dixon G. H., Hanes C. S. 1955; Quantitative chromatographic methods for the study of enzymic transpeptidation reactions. Canad. J. Biochem 33:416
    [Google Scholar]
  6. Davis B.D. 1952a; Aromatic biosynthesis. IV. Preferential conversion, in incompletely blocked mutants, of a common precursor of several metabolites. J. Bad 64:729
    [Google Scholar]
  7. Davis B. D. 1952b; Biosynthetic interrelations of lysine, diaminopimelic acid and threonine in mutants of Escherichia coli . Nature, Lond 169:534
    [Google Scholar]
  8. Davis B. D. 1953 Symposium on Microbial Metabolism. 5th Congr. ini. Microbiol p. 28
    [Google Scholar]
  9. Francis G. E., Mulligan W., Wormall A. 1954 Isotopic Tracers London: The Athlone Press;
    [Google Scholar]
  10. Gilvarg C. 1960; Biosynthesis of diaminopimelic acid. Fed. Proc 19:948
    [Google Scholar]
  11. Moore S., Stein S. W. 1954; A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J. biol. Chem 211:907
    [Google Scholar]
  12. Morrison R. I. 1953; The isolation of l-pipecolinic acid from Trifolium repens . Biochem. J 53:474
    [Google Scholar]
  13. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. T., Britten R. J. 1955; Studies of biosynthesis in Escherichia coli . Publ. Cameg. Instn no. 607
    [Google Scholar]
  14. Siddiqi M. S. H., Kozloff L. M., Putnam F. W., Evans E. A. 1952; Biochemical studies of virus reproduction. IX. Nature of the host cell contributions. J. biol. Chem 199:165
    [Google Scholar]
  15. Skerman V. B. D. 1959 A Guide to the Identification of the Genera of Bacteria Baltimore: Williams and Wilkins;
    [Google Scholar]
  16. Smith I. 1958 Chromatographic Techniques London: William Heinemann;
    [Google Scholar]
  17. Thompson F. J., Morris C. J. 1959; Determination of amino acids from plants by paper chromatography. Analyt. Chem 31:1031
    [Google Scholar]
  18. Thompson F. J., Morris C. J., Gering R. K. 1959; Purification of plant amino acids for paper chromatography. Analyt. Chem 31:1028
    [Google Scholar]
  19. Work E. 1955 A Symposium on Amino Acid Metabolism p. 462 Ed. by McElroy W. D., Glass B. Baltimore: Johns Hopkins;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-27-1-41
Loading
/content/journal/micro/10.1099/00221287-27-1-41
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error