1887

Abstract

SUMMARY: Samples of a liquid culture of Proteus L9 were transferred to agar blocks containing the same medium as the liquid culture. Photomicrographs were made of the slide cultures thus obtained, and the diameters of a large number of individual L elements determined. The measurements indicated that the elements of the L cultures studied could be assigned to two classes (I and II) with regard to their size. The elements of class I had an average diameter of 0.33 μ (standard deviation 0.071; standard error of the mean 0.006 μ); the corresponding figures for class II were 0.94, 0.21 and 0.02 μ, respectively. The growth of individual L elements in the slide cultures was followed during incubation at 30°. Only elements having a diameter > 0.6 to 0.7 μ, i.e. elements belonging to class II, enlarged measurably or formed microcolonies. Calculations based on measurements made on L elements growing on streak plates confirmed these observations.

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/content/journal/micro/10.1099/00221287-27-2-241
1962-02-01
2024-05-02
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References

  1. Abrams R. Y. 1955; A method for the cultivation of L forms in liquid media. J. Bad 70:251
    [Google Scholar]
  2. Kellenberger E., Liebermeister K., Bonifas Y. 1956; Studien zur L-form der Bakterien II. Z. Naturf 116:206
    [Google Scholar]
  3. Klieneberger-Nobel E. 1956; über die Wesensverschiedenheit der peri-pneumonie-ahnlichen Organismen und der L-phase der Bakterien. Zbl. Bakt. (1 Abt. Orig.) 165:329
    [Google Scholar]
  4. Kheneberger-Nobel E. 1960; L-forms of bacteria. In The Bacteria Ed. Gunsalus I. C., Stanier R. Y. Vol. I p. 443 New York and London: Academic Press Inc;
    [Google Scholar]
  5. Knöll H. 1944; Zur Anwendung der Phasenkontrastmikroskopie in der Biologie. Zeiss Nachr 5:38
    [Google Scholar]
  6. Rada B. 1959; Filtrierbarkeit der L-Phase und der globulären Form der Bakterien. Zbl. Bakt. (1 Abt. Orig.) 176:85
    [Google Scholar]
  7. Roux J. 1960; La multiplication des formes L. Ann. Inst. Pasteur 99:286
    [Google Scholar]
  8. Taubeneck U. 1959; New grid-replica for precise localization in slide cultures. J. Bad 77:506
    [Google Scholar]
  9. Tulasne R., Lavillaureix J. 1958; Filtration et ultrafiltration d’une souche de forme L fixées. C.R. Acad. Sci., Paris 246:2396
    [Google Scholar]
  10. Tulasne R., Minck R., Kirn A., Krembel J. 1960; Délimitation de la notion des formes L des bacteries: protoplastes, spheroplastes et formes L. Ann. Inst. Pasteur 99:859
    [Google Scholar]
  11. Weibull C., Beckman H. 1961; Chemical and metabolic properties of various elements found in cultures of a stable Proteus L form. J. gen. Microbiol 24:379
    [Google Scholar]
  12. Weibull C., Lundin B.-M. 1961; Factors influencing the growth of a stable L form of Proteus mirabilis . J. Bad 81:812
    [Google Scholar]
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