1887

Abstract

SUMMARY: The conspicuous, large, high refractive index, sudanophilic granules of appeared to be aggregations of polymeric β-hydroxybutyric acid, probably closely associated with the cytoplasm. They became more conspicuous as the organism aged, provided that carbohydrate was in excess. A well-grown culture contained 40–50% polymer, based on cell dry weight. Relatively large cytoplasmic granules (50–80 mμ) were a feature of this organism whether in fixed and sectioned cells or in material shadowed after mechanical disintegration.

Classical ‘double’ (? lipoprotein) membranes were demonstrated both for the cell wall and the cytoplasmic membrane. Carefully fixed and embedded material often showed an accumulation of material between the two double membranes, especially at one or both ends, without any evidence of gross damage in the sectioned organisms.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-29-3-551
1962-11-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/29/3/mic-29-3-551.html?itemId=/content/journal/micro/10.1099/00221287-29-3-551&mimeType=html&fmt=ahah

References

  1. Beer M. 1960; Disposition of membranes in Alcaligenes faecalis. J. Bact. 80 659
    [Google Scholar]
  2. Burdon K. L. 1946; Fatty material in bacteria and fungi revealed by staining dried fixed slide preparations. J. Bact. 52 665.
    [Google Scholar]
  3. Doudoroff M., Stanier R. Y. 1959; The role of poly-β-hydroxybutyric acid in the assimilation of organic carbon by bacteria. Nature, Lond. 183 1440
    [Google Scholar]
  4. Duncan R. E. B., Porteus J. W. 1953; The identification and determination of the lower straight chain fatty acids by paper partition chromatography. Analyst 78 641
    [Google Scholar]
  5. Forsyth W. C. G., Hayward A. C., Roberts J. B. 1958; Occurrence of poly-β-hydroxybutyric acid in aerobic Gram-negative bacteria. Nature, Lond. 182 800
    [Google Scholar]
  6. Humphrey B. A., Vincent J. M. 1962; Calcium in the cell walls of Rhizobium trifolii. J. gen. Microbiol. 29 557
    [Google Scholar]
  7. Kellenberger E., Ryter A. 1958; The cell wall and cytoplasmic membrane of Escherichia coli. J. biophys. biochem. Cytol. 4 323
    [Google Scholar]
  8. Kepes A., Peaud-Lenoel C. 1952; Sur les proprietes et la constitution des lipides β-hydroxy-butyriques. C.R. Acad. Sci., Paris 234 756
    [Google Scholar]
  9. Lemoigne M. 1925; fitude sur 1’autolyse microbienne; acidification par formation d’acide β-hydroxy-butyrique. Ann. Inst. Pasteur 39 144
    [Google Scholar]
  10. Lemoigne M. 1946; Fermentation β-hydroxy-butyrique. Helv. chim. Acta 29 1303
    [Google Scholar]
  11. Lemoigne M., Sanchez G., Girard H. 1943; Sur la characterization de la fermentation β-hydroxy-butyrique. Ann. Inst. Pasteur 69 187
    [Google Scholar]
  12. Macrae R. M., Wilkinson J. F. 1958; Polyβ-hydroxy-butyrate metabolism in washed suspensions of Bacillus cereus and Bacillus megaterium. J. gen. Microbiol. 19 210
    [Google Scholar]
  13. Merrick J. M., Doudoroff M. 1961; Enzymatic synthesis of poly β-OH-butyric acid in bacteria. Nature, Lond. 190 1215
    [Google Scholar]
  14. North R. J. 1961; Method of reaching the membrane systems of micro-organisms. Nature, Lond. 190 1215
    [Google Scholar]
  15. Robertson J. D. 1955; The ultra-structure of adult vertebrate peripheral myelinated nerve fibres in relation to myelinogenesis. J. biophys. biochem. Cytol. 1 271
    [Google Scholar]
  16. Robertson J. D. 1958; Structural alterations in nerve fibres produced by hypotonic and hypertonic solutions. J. biophys. biochem. Cytol. 4 349
    [Google Scholar]
  17. Schlegel H. G., Gottschalk G., von Bartha R. 1961; Formation and utilization of poly-β-hydroxy-butyric acid by knallgas bacteria (Hydrogenomonas). Nature, Lond. 191 1463
    [Google Scholar]
  18. Smithies W. R., Gibbons N. E., Bayley S. 1955; The chemical composition of the cell and cell wall of some halophilic bacteria. Canad. J. Microbiol. 1 605
    [Google Scholar]
  19. Vincent J. M. 1962; Requirements of rhizobium for calcium and magnesium. J. gen. Microbiol, (in the Press)
    [Google Scholar]
  20. Williamson D. H., Wilkinson J. F. 1958; Isolation and estimation of the poly-β-OH-butyrate inclusions in Bacillus species. J. gen. Microbiol. 19 198
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-29-3-551
Loading
/content/journal/micro/10.1099/00221287-29-3-551
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error