1887

Abstract

Summary: Influenza and related viruses were studied by a new method in which virus is adsorbed on the membranes of laked fowl red cells for examination in the electron microscope. The numbers of virus particles adsorbed per unit area of red-cell membrane were estimated from direct counts in micrographs of palladium-shadowed and unshadowed specimens. There was a definite saturation level of adsorption for each strain of virus, the value varying also according to the particular batch of cells and their age after storage at 0°. For subsaturation conditions the number of particles adsorbed was proportional to the concentration of virus and the concentration of cells, and a function both of time of contact and temperature. The relationships were complicated by the fact that elution began before adsorption was complete.

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/content/journal/micro/10.1099/00221287-3-2-298
1949-05-01
2024-05-03
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References

  1. Bang F.B. 1946; Filamentous forms of Newcastle virus. Proc. Soc. exp. Biol., N.Y. 63:5
    [Google Scholar]
  2. Bang F.B. 1947; Newcastle virus: conversion of spherical forms to filamentous forms. Proc. Soc. exp. Biol., N.Y. 64:135
    [Google Scholar]
  3. Bang F.B. 1948; Studies on Newcastle disease virus. I. An evaluation of the method of titration. II. Behaviour of the virus in the embryo. III. Characters of the virus itself with particular reference to electron microscopy. J. exp. Med. 88:251
    [Google Scholar]
  4. Beard J.W. 1948; Review: purified animal viruses. J. Immunol. 58:49
    [Google Scholar]
  5. Beveridge W.I.B., Burnet F.M. 1946; The cultivation of viruses and rickettsiae in the chick embryo. Spec. Rep. Ser. med. Res. Coun., Lond.256
    [Google Scholar]
  6. Burnet F.M. 1948; The initiation of cellular infection by influenza and related viruses. Lancet i:7
    [Google Scholar]
  7. Cunha R., Weil M.L., Beard D., Taylor A.R., Sharp D.G., Beard J.W. 1947; Purification and characters of the Newcastle disease virus (California strain). J. Immunol. 55:69
    [Google Scholar]
  8. Dawson I.M., Elford W.J. 1948; Electron microscope studies on the interaction of certain viruses with fowl red-cell membranes. Lond.: 16363
    [Google Scholar]
  9. Dounce A.L., Lan T.H. 1943; Isolation and properties of chicken erythrocyte nuclei. Science 97:584
    [Google Scholar]
  10. Elford W.J., Chu C.M., Dawson I.M., Dudgeon J.A., Fulton F., Smiles J. 1947; Physical properties of the viruses of Newcastle disease, fowl plague and mumps. Brit.J. exp. Path. 29:590
    [Google Scholar]
  11. Hanig M. 1948; Electrokinetic change in human erythrocytes during adsorption and elution of PR8 influenza virus. Proc. Soc. exp. Biol., N.Y. 68:385
    [Google Scholar]
  12. Heinmets F. 1948; Studies with the electron microscope on the interaction of red cells and influenza virus. J. Bact. 55:823
    [Google Scholar]
  13. Hirst G.K. 1941; The agglutination of red cells by allantoic fluid of chick embryos infected with influenza virus. Science 94:22
    [Google Scholar]
  14. Hirst G.K. 1942; Adsorption of influenza haemagglutinins and virus by red blood cells. J. exp. Med. 76:195
    [Google Scholar]
  15. Lauffer M.A., Stanley W.M. 1944; Biophysical properties of preparations of PR 8 influenza virus. J. exp. Med. 80:531
    [Google Scholar]
  16. McClelland L., Hare R. 1941; The adsorption of influenza virus by red cells and a new in vitro method of measuring antibodies for influenza virus. Canad. publ. Hlth J. 32:530
    [Google Scholar]
  17. Mosley V.M., Wyckoff R.W.G. 1946; Electron micrography of the virus of influenza. Nature; Lond.: 157263
    [Google Scholar]
  18. Salk J.E. 1944; A simplified procedure for titrating haemagglutinating capacity of influenza-virus and the corresponding antibody. J. Immunol. 49:87
    [Google Scholar]
  19. Sharp D.G., Taylor A.R., Mclean I.W. Jr. Beard D., Beard J.W. 1944; Sedimentation velocity and electron micrographic studies of influenza viruses A (PR 8 strain) and B (Lee strain) and the swine influenza virus. J. biol. Chem. 156:585
    [Google Scholar]
  20. Weil M.L., Beard D., Sharp D.G., Beard J.W. 1948; Purification and sedimentation and electron micrographic characters of the mumps virus. Proc. Soc. exp. Biol., N.Y. 68:309
    [Google Scholar]
  21. Wolpers C. 1941; Zur Fein Struktur der Erythrocytenmembran. Naturwissen- schaften 29:416
    [Google Scholar]
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