1887

Abstract

SUMMARY

Methionine or ethionine, although capable of serving as sole nitrogen source for , cannot replace or spare the vitamin B nutrient requirement for the growth of this flagellate. In the presence of vitamin B methionine counteracted the inhibition of growth exerted by some vitamin B analogues which are substituted at the benzimidazole part of the molecule. No such effect was observed against the inhibition by certain other analogues. Analogue FIII (α-(5-hydroxybenzimidazolyl)-cobamide cyanide) replaced vitamin B in the presence of methionine, and to a lesser degree also in the presence of other methyl donors such as methylmethionine sulphonium iodide, dimethylpropiothetin, choline chloride and betaine. The conclusion drawn is that for analogue FIII is capable of replacing vitamin B in all the metabolic pathways other than methyl-group synthesis. For the latter process, the benzimidazole methyl groups present in vitamin B and analogue FIII (α-(5-methoxy-benzimidazolyl)-cobamide cyanide) are indispensable.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-31-2-203
1963-05-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/31/2/mic-31-2-203.html?itemId=/content/journal/micro/10.1099/00221287-31-2-203&mimeType=html&fmt=ahah

References

  1. Davis B. D., Mingioij E. S. 1950; Mutants o. Escherichia coli requiring methionine or vitamin B12. J. Bact 60:17
    [Google Scholar]
  2. Dinning J. S., Young R. S. 1960; Some effects of folic acid and vitamin B12 on nucleic acid metabolism in Lactobacillus leichmannii. J. biol. Chem 235:3008
    [Google Scholar]
  3. Downing M., Schweigert B. S. 1956a; Role of vitamin B12 in nucleic acid metabolism. III. Preparation of C14 uniformly labeled pyrimidine deoxynucleosides. J. biol. Chem 220:513
    [Google Scholar]
  4. Downing M., Schweigert B. S. 1956b; Role of vitamin B12 in nucleic acid metabolism. IV. Metabolism of C14-labeled thymidine by Lactobacillus leichmannii. J. biol. Chem 220:521
    [Google Scholar]
  5. Droop M. R. 1954; Cobalamin requirement in Chrysophyceae. Nature, Lond 174:520
    [Google Scholar]
  6. Ford J. E. 1958; B12 vitamins and growth of the flagellate Ochromonas malhamensis. J. gen. Microbiol 19:161
    [Google Scholar]
  7. Gross D., Tarver H. 1955; Studies on ethionine. IV. The incorporation of ethionine into the proteins of Tetrahymena. J. biol. Chem 217:169
    [Google Scholar]
  8. Guest J. R., Helleiner C. W., Cross M. J., Woods D. D. 1960; Cobalamin and the synthesis of methionine by ultrasonic extracts o. Escherichia coli. Biochem. J 76:396
    [Google Scholar]
  9. Heinrich H. C. editor 1957 Vitamin i?12 und Intrinsic Factor. 1. Europäisches Symposion, Hamburg 1956: Stuttgart: Ferdinand Enke Verlag;
    [Google Scholar]
  10. Heinrich H. C. editor 1962 Vitamin J3ia und Intrinsic Factor. 2. Europäisches Symposion, Hamburg 1961: Stuttgart: Ferdinand Enke Verlag;
    [Google Scholar]
  11. Helleiner C. W., Kisliuk R. L., Woods D. D. 1957; Cobalamin and the synthesis of methionine by cell free extracts o. Escherichia coli. J. gen. Microbiol 18:23
    [Google Scholar]
  12. Hutner S. H., Provasoli L., Filfus J. 1953; Nutrition of some phagotrophic freshwater Chrysomonads. Ann. N.Y. Acad. Sci 56:852
    [Google Scholar]
  13. Johnson B. C, Holdsworth E. S., Porter J. W. G., Kon S. K. 1957; Vitamin B12 and methyl-group synthesis. Brit. J. Nutr. 2:313
    [Google Scholar]
  14. Manson L. A. 1960; Vitamin B18 and deoxyribose synthesis in Lactobacillus leichmannii. J. biol. Chem 235:2955
    [Google Scholar]
  15. Rahat M., Reich K. 1963; The B12 vitamins and growth of the flagellate Prymnesium parvum (Chrysomonadina). J. gen. Microbiol 31:195
    [Google Scholar]
  16. Rotberg M. 1958; The thiamine requirements o. Prymesium parvum (Chrysomonadina). Bull. Research Council Israel 7b:199
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-31-2-203
Loading
/content/journal/micro/10.1099/00221287-31-2-203
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error