1887

Abstract

SUMMARY: Leprosy bacilli have not been cultivated with certainty in any cell-free medium, but the medium now described consistently supported considerable elongation of the organisms of murine leprosy (), though without evidence of multiplication. The mean length of the bacilli doubled in about the generation time obtaining in host cells (7-14 days) and quadrupled before the bacilli became degenerate after about 2 months. The obligate acidity and other factors concerned in this growth were investigated, and are discussed in relation to the problem of the extracellular cultivation of leprosy bacilli.

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/content/journal/micro/10.1099/00221287-32-1-43
1963-07-01
2024-05-02
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References

  1. Bekierkunst A. 1957; The effect of pH of the medium on the growth of tubercle bacilli. Schweiz. Z. Path. Bakt 20:312
    [Google Scholar]
  2. Chang Y. T. 1960; Changes in the length of Mycobacterium lepraemurium in the evolution of murine leprosy in mice. Amer. Rev. Resp. Dis 82:409
    [Google Scholar]
  3. Chang Y. T. 1961; The mouse macrophage as host cell for. Mycobacterium lepraemurium. Trans. Symposium on Research in Leprosy (Baltimore) p 118 Washington: Leonard Wood Memorial;
    [Google Scholar]
  4. Dubos R. J., Davis B. D. 1946; Factors affecting the growth of tubercle bacilli in liquid medium. J. exp. Med 83:409
    [Google Scholar]
  5. Eddy B. E. 1937; Attempted cultivation of. Mycobacterium leprae. Int. J. Leprosy 5:31
    [Google Scholar]
  6. Garbutt E. W., Rees R. J. W., Barr Y. M. 1962; Growth of Mycobacterium lepraemurium maintained in cultures of rat fibroblasts. J. gen. Microbiol 27:259
    [Google Scholar]
  7. Gray C. T. 1952; The respiratory metabolism of murine leprosy bacilli. J. Bact 64:305
    [Google Scholar]
  8. Hart D’Arcy P. 1958; A mycobactin-containing liquid medium for the study of Mycobacterium johnei . J. Path. Bad 76:205
    [Google Scholar]
  9. Hart P., D’Arcy & Rees R. J. W. 1960; Effect of Macrocyclon in acute and chronic pulmonary tuberculous infection in mice as shown by viable and total bacterial counts. Brit. J. exp. Path 41:414
    [Google Scholar]
  10. Hart D’Arcy P., Rees R. J. W., Valentine R. C. 1962a; The length of Mycobacterium lepraemurium recovered from different animal species and tissues after experimental infection. J. Path. Bact 83:153
    [Google Scholar]
  11. Hart P., D’Arcy, Rees R. J. W., Valentine R. C. 1962b; Isoniazid resistant and dependent strains of Mycobacterium lepraemurium studied in vivo and in vitro . J. Path. Bact 84:105
    [Google Scholar]
  12. Hart D’Arcy P., Valentine R. C. 1960; Elongation of a leprosy bacillus (Mycobacterium lepraemurium) in a cell-free medium. Nature, Lond 185:58
    [Google Scholar]
  13. Hedgecock L. W., Costello R. L. 1962; Utilization of nitrate by pathogenic and saprophytic mycobacteria. J. Bact 84:195
    [Google Scholar]
  14. Hilson G. R. F., Elek S. D. 1957; Intratesticular multiplication of Mycobacterium lepraemurium in normal and suramin-treated animals. Int. J. Leprosy 25:380
    [Google Scholar]
  15. McFadzean J. A., Valentine R. C. 1959; The value of acridine orange and of electron microscopy in determining the viability of. Mycobacterium lepraemurium. Trans. Roy. Soc. trop. Med. Hyg 53:414
    [Google Scholar]
  16. Nicolay N. De 1585 The Navigations into Turkie p 33 Transl. T. Washington. London: T. Dawson;
    [Google Scholar]
  17. Rees R. J. W., Garbutt E. W. 1962; Studies on Mycobacterium lepraemurium in tissue culture. I. Multiplication and growth characteristics in cultures of rat fibroblasts. Brit. J. exp. Path 43:221
    [Google Scholar]
  18. Rees R. J. W., Valentine R. C. 1962; A modified Ziehl-Neelsen staining method for identifying dead Mycobacterium lepraemurium. Int . J. Leprosy (in the Press)
    [Google Scholar]
  19. Valentine R. C. 1962; Quantitative electron microscopy of leprosy bacilli. Brit. med. Bull 18:242
    [Google Scholar]
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