1887

Abstract

Summary: Thick suspensions of spores of did not germinate completely in nutrient media because the first spores to germinate released a substance which inhibited germination of the remainder. The inhibitory substance was identified as -alanine by chromatography, high voltage electrophoresis, and its destruction by -amino acid oxidase. When C--alanine was used to induce germination of spores, the resulting inhibitory -alanine was C-labelled with the same specific activity as the C--alanine used. Auto-inhibition of spore germination is therefore due to racemization of exogenous -alanine, which forms sufficient -alanine to arrest the germination process in thick suspensions of spores.

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/content/journal/micro/10.1099/00221287-35-2-229
1964-05-01
2024-05-04
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