1887

Abstract

SUMMARY: The base composition of DNA preparations from 12 strains of Streptomyces and 1 strain of Nocardia were determined from their denaturation temperature ( ) and buoyant density.

The % GC (guanine+cytosine) contents ranged from 74·4 to 78·5 (from m) and from 69·4 to 73·4 (from buoyant density). The correlation between the two sets of data and the found differences are discussed. The range of pH values which did not affect and the degree of hyperchromic effect have been related to the ionic strength of the solvent. With 0·01 M-phosphate as solvent the pH indifference range was narrower and the hyperchromic effect smaller than with 0·2M-Na solvent.

Though the difference between the maximum and minimum % GC in Streptomyces is sufficient to distinguish the respective strains, within the extreme values there is a continuous progression of base composition which does not permit taxonomic divisions to be made on the basis of overall % GC alone.

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/content/journal/micro/10.1099/00221287-38-2-243
1965-02-01
2024-04-27
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References

  1. Alacevic M. 1963; Interspecific recombination in Streptomyces. Nature, Lond. 197:1323
    [Google Scholar]
  2. Belozersky A. N., Spirin A. S. 1960 In The Nucleic Acids vol. 3: p. 147 Ed. by Chargaff E., Davidson J. N. London and New York: Academic Press.;
    [Google Scholar]
  3. Bradley S. G., Lederberg J. 1956; Heterokaryosis in Streptomyces. J. Bad. 72:219
    [Google Scholar]
  4. De Ley J., Schell J. 1963; Deoxyribonucleic acid base composition of acetic acid bacteria. J. gen. Microbiol. 33:243
    [Google Scholar]
  5. Hill L. R., Silvestri L. G. 1963; Rapporto delle basi del DNA e analisi tassometrica di alcuni streptomiceti. Boll. Soc. it. Biol. sper. 39:1864
    [Google Scholar]
  6. Jarai M. 1961; Transformation in Streptomyces. Acta microbiol. Acad. Sci. hung. 8:81
    [Google Scholar]
  7. Jones L. A., Bradley S. G. 1963; Thermal denaturation of deoxyribonucleic acid from actinomycetes and actinophages. Bajct. Proc. p. 148
    [Google Scholar]
  8. Lee K. Y., Wahl R., Barbu E. 1956; Contenu en bases puriques et pyrimidiques des acides désoxyribonucleiques des bactéries. Annls Inst. Pasteur, Paris 91:212
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. mol. Biol. 3:208
    [Google Scholar]
  10. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. mol. Biol. 5:109
    [Google Scholar]
  11. Marmur J., Falkow S., Mandel M. 1963; New approaches to bacterial taxonomy. Ann. Rev. Microbiol. 17:329
    [Google Scholar]
  12. Meselson M., Stahl F. W., Vinograd J. 1957; Equilibrium sedimentation of macromoleeules in density gradients. Proc. not. Acad. Sci., Wash. 43:581
    [Google Scholar]
  13. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. mol. Biol. 4:430
    [Google Scholar]
  14. Silvestri L. G., Turri M., Hill L. R., Gilardi E. 1962; A quantitative approach to the systematics of actinomycetes based on overall similarity. In Microbial Classification. Symp. Soc. gen. Microbiol. 12:333
    [Google Scholar]
  15. Sueoka N. 1961; Variation and heterogeneity of base composition of deoxyribonucleic acids: a compilation of old and new data. J. mol. Biol. 3:31
    [Google Scholar]
  16. Ts’o P. O. P., Helmkamp G. K., Sander C. 1962; Interaction of nucleosides and related compounds with nucleic acids as indicated by the change of helix-coil transition temperature. Proc. nat. Acad. Sci., Wash. 48:686
    [Google Scholar]
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