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Some Carbon-Dioxide Requiring Mutants of Neurospora crassa
Abstract
SUMMARY
This paper describes some Neurospora mutants which grow on minimal medium when the gas-phase contains 30 % CO2 (v/v); they are referred to as ‘CO2 mutants’. One is an arginine mutant (arg-2; 33442) having a mutation at a different locus from the other arginine CO2 mutant (arg-3; 30300); a second (arg-11; 30820) requires arginine + a purine + a pyrimidine for growth in the absence of CO2; a third (44601) requires arginine + a purine + a pyrimidine + a carboxylic acid for growth in the absence of CO2. The mutations in mutants 30820 and 44601 are allelic and the carboxylic acid requirement of mutant 44601 is attributable to its genetic background. The remaining CO2 mutants described here were isolated directly from wild-type Neurospora, and their nutritional requirements in the absence of CO2 subsequently determined. Two of these mutants, which are allelic, are adenine-requiring mutants sited at the adenine-3 locus, although the adenine-3A and -3B mutants 38709 and Y 112-M2 are not CO2 mutants. Another two of these new CO2 mutants, which may be allelic, require carboxylic acids in the absence of CO2; these mutants are similar in their nutritional requirements to the succinate mutants described by Lewis (1948) and Strauss (1956) .
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© Society for General Microbiology 1965