1887

Abstract

SUMMARY

Mitochondria isolated from protoplasts of conidia, young germinated conidia, and branched mycelia of , have an active oxidative phosphorylation system which showed no variation in the three stages of development studied ; succinate dehydrogenase also did not alter. Cytochrome oxidase activity showed a marked increase at the time of conidial germination; this was not maintained during further growth. An electron microscope study of the conidia showed them to have complex mitochondria analogous to those seen in older mycelial hyphae. A large accumulation of lipid material and extensive vacuolization was observed in these conidia. A rudimentary reticular system with numerous ribosomes lying free in this membrane system were observed. Macroconidia were usually multinucleate; up to 10 nuclei were observed. The outer conidial wall was completely digested by snail extract ( digestive juice) and was structurally identical with that of the hyphae as seen with the fixation and embedding conditions used.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-39-1-85
1965-04-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/39/1/mic-39-1-85.html?itemId=/content/journal/micro/10.1099/00221287-39-1-85&mimeType=html&fmt=ahah

References

  1. Huebschman C. 1952; A method for varying the average number of nuclei in the conidia of Neurospora crassa . Mycologia 44:599
    [Google Scholar]
  2. Hunter F. E. Jr. 1955; Coupling of phosphorylation with oxidation. Meth. Enzymol 2:610
    [Google Scholar]
  3. Kinsky S. C. 1962; Effect of polyene antibiotics on protoplasts of Neurospora crassa . J. Bact 83:351
    [Google Scholar]
  4. Lindberg O., Ernster L. 1956; Determination of organic phosphorus compounds by phosphate analysis. Meth. biochem. Analysis 3:3
    [Google Scholar]
  5. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  6. Luft J. H. 1956; Permanganate: a new fixative for electron microscopy. J. biophys. biochem. Cytol 2:799
    [Google Scholar]
  7. Ryan F. J., Beadle G. W., Tatum E. L. 1943; The tube method of measuring the growth rate of Neurospora. Amer. J. Bot 30:784
    [Google Scholar]
  8. Sabatini D., Miller F., Barnett R. 1964; Aldehyde fixation for morphological and enzyme histochemical studies with the electron microscope. J. Histochem. Cytochem 12:57
    [Google Scholar]
  9. Shatkin A. J., Tatum E. 1959; Electron microscopy of Neurospora crassa mycelia. J. biophys. biochem. Cytol 6:423
    [Google Scholar]
  10. Singer T. P., Kearney E. B. 1957; Determination of succinic dehydrogenase activity. Meth. biochem. Analysis 4:307
    [Google Scholar]
  11. Slater E. C. 1949; The measurement of the cytochrome oxidase activity of enzyme preparations. Biochem. J 44:305
    [Google Scholar]
  12. Turian G. 1962; Deficiences du metabolisme oxydatif et differenciation sexuelle chez Allomyces et Neurospora. Activité d’une DPN-deshydrogenase lactique chez Allomyces. Pathologia Microbiol 23:687
    [Google Scholar]
  13. Yotsuyanagi M. Y. 1959; Étude au microscope electronique des coupes ultrafine de la levure. C. r. hebd. Séanc. Acad. Sci., Paris 248:274
    [Google Scholar]
  14. Yotsuyanagi M. Y. 1962; Étude sur le chondriome de la levure. I. Variation de l’ultrastrueture du chondriome au cours du cycle de la croissance aerobie. J. infrastructure Res 7:121
    [Google Scholar]
  15. Zalokar M. 1959; Enzyme activity and cell differentiation in Neurospora. Amer. J. Bot 46:555
    [Google Scholar]
  16. Zalokar M. 1961; Electron microscopy of centrifuged hyphae of Neurospora. J. biophys. biochem. Cytol 9:609
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-39-1-85
Loading
/content/journal/micro/10.1099/00221287-39-1-85
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error