1887

Abstract

SUMMARY

The genetic control of histidine biosynthesis in was examined by an analysis of 66 histidine-dependent mutants by using stable and abortive transduction. These mutants, which previously had been differentiated into classes according to their biochemical characteristics, all occupied sites within a single linkage group, referred to as the histidine region. This region has been separated into six gene loci on the basis of complementation studies and the biochemical characteristics of the mutants. The order of genes within the histidine region, , was determined from the results of reciprocal transductions to wild type and donor type. The order of mutant sites was also linearly related to the ability of these mutants to form histidine-independent transductants when infected with phage prepared on the parent strain. This observation, and the inequalities in reciprocal transduction frequencies obtained in inter-mutant transductions, support the hypothesis that all donor fragments which participate in transductions involving the histidine region are identical, and that the histidine region is located extremely close to one terminus of the donor fragment. Intergenic complementation occurred among mutants of all gene loci; in addition, three complementation units within the gene and two complementation units within the gene were detected.

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/content/journal/micro/10.1099/00221287-39-2-195
1965-05-01
2024-05-05
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References

  1. Ames B. N., Hartman P. E. 1962; Genes, enzymes, and control mechanisms in histidine biosynthesis. In The Molecular Basis of Neoplasia322–45 Austin, Texas: University of Texas Press;
    [Google Scholar]
  2. Ames B. N., Hartman P. E. 1963; The histidine operon. Cold Spr. Harb. Symp. quant. Biol 28:349
    [Google Scholar]
  3. Ephrati-Elizur E., Srinivasan P. R., Zamenhof S. 1961; Genetic analysis, by means of transformation, of histidine linkage groups in Bacillus subtilis . Proc. natn. Acad. SciU. S. A 4756
    [Google Scholar]
  4. Haas F., Mitchell M. B., Ames B. N., Mitchell H. K. 1952; A series of histidineless mutants of Neurospora crassa. Genetics 37:217
    [Google Scholar]
  5. Hartman P. E. 1956; Linked loci in the control of consecutive steps in the primary pathway of histidine synthesis in Salmonella typhimurium. Genetic Studies with Bacteria, Publ. Carneg. Instn61235
    [Google Scholar]
  6. Hartman P. E., Hartman Z., Serman D. 1960; Complementation mapping by abortive transduction of histidine requiring Salmonella mutants. J. gen. Microbiol 22:354
    [Google Scholar]
  7. Hartman P. E., Loper J. C., Serman D. 1960; Fine structure mapping by complete transduction between histidine requiring Salmonella mutants. J. gen. Microbiol 22:323
    [Google Scholar]
  8. Kloos W. E., Pattee P. A. 1965; A biochemical characterization of histidine-dependent mutants of Staphylococcus aureus. J. gen. Microbiol 39:185
    [Google Scholar]
  9. Loper J. C. 1960; Gene–enzyme relationships in histidine biosynthesis in Salmonella. Ph.D. Thesis Baltimore, Maryland: The Johns Hopkins University;
    [Google Scholar]
  10. Loper J. C. 1961; Enzyme complementation in mixed extracts of mutants from the Salmonella histidine B locus. Proc. natn. Acad. SciU. S. A 471440
    [Google Scholar]
  11. Martin R. G. 1963; The first enzyme in histidine biosynthesis: the nature of feedback inhibition by histidine. J. biol. Chem 238:257
    [Google Scholar]
  12. Ozeki H. 1959; Chromosome fragments participating in transduction in Salmonella typhimurium. Genetics 44:457
    [Google Scholar]
  13. Pattee P. A., Baldwin J. N. 1961; Transduction of resistance to chlortetracycline and novobiocin in Staphylococcus aureus. J. Bact 82:875
    [Google Scholar]
  14. Pattee P. A., Baldwin J. N. 1962; Transduction of resistance to some macrolide antibiotics in Staphylococcus aureus. J. Bact 84:1049
    [Google Scholar]
  15. Smith D. W. E., Ames B. N. 1964; Intermediates in the early steps of histidine biosynthesis. J. biol. Chem 239:1848
    [Google Scholar]
  16. Webber B., Case M. 1960; Genetic and biochemical studies of histidine-requiring mutants of Neurospora crassa. I. Classification of mutants and characterizations of mutant groups. Genetics 45:1605
    [Google Scholar]
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