1887

Abstract

SUMMARY: The electrokinetic behaviour of intact conidia and cell walls of was studied using a micro-electrophoresis technique. By chemical and enzyme treatments it has been established that amino, carboxyl and phosphate groups are integral components of the spore surface; acid phosphate groups, however, were not found on the surface of washed cell walls. The fungicide dodine acetate reduced the negative charge on conidia to zero and, with increasing concentration, gave a positive charge to the spores: at lower fungicide concentrations the negative charge on the surface of cell walls and stabilized protoplasts was also neutralized. These results are consistent with an ionic reaction between the dodine cation and the carboxyl and phosphate groups of the cell. There was no evidence that the toxic reaction between dodine acetate and conidia is located on the spore surface—the spores were completely killed before there was a perceptible reduction in electrophoretic mobility.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-48-1-147
1967-07-01
2024-05-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/48/1/mic-48-1-147.html?itemId=/content/journal/micro/10.1099/00221287-48-1-147&mimeType=html&fmt=ahah

References

  1. Aronson J. M. 1965; The cell wall. In The Fungi Ed. by Ainsworth G. C., Sussman A. S. 1 p 49 New York: Academic Press;
    [Google Scholar]
  2. Best G. K., Durham N. N. 1965; Vancomycin adsorption to Bacillus subtilis cell walls. Archs Biochem. Biophys 111:685
    [Google Scholar]
  3. De Boer J. Th., Backer H. J. 1954; A new method for the preparation of diazomethane. Reel Tray. chim. Pays-Bas Belg 73:229
    [Google Scholar]
  4. Douglas H. W. 1959; Electrophoretic studies on bacteria. V. Interpretation of the effects of pH and ionic strength on the surface charge borne by B. subtilis spores with some observations on other organisms Trans. Faraday Soc 55:850
    [Google Scholar]
  5. Douglas H. W., Collins A. E., Parkinson D. 1959; Electric charge and other surface properties of some fungal spores. Biochim. biophys. Acta 33:535
    [Google Scholar]
  6. Dyar M. T., Ordal E. J. 1946; Electrokinetic studies on bacterial surfaces. I. The effects of surface-active agents on the electrophoretic mobilities of bacteria J. Bact 51:149
    [Google Scholar]
  7. Dyke K. G. H. 1964; The chemical composition of the cell wall of the yeast, Nadsonia elongata. Biochim. biophys. Acta 82:374
    [Google Scholar]
  8. Eddy A. A., Rudin A. D. 1958; The structure of the yeast ceil wall. I. Identification of charged groups at the surface Proc. R. Soc B 148:419
    [Google Scholar]
  9. Gittens G. J., James A. M. 1960; An improved microelectrophoresis apparatus and technique for studying biological cell surfaces. Analyt. Biochem 1:478
    [Google Scholar]
  10. Gittens G. J., James A. M. 1963; Some physical investigations of the behaviour of bacterial surfaces. VI. Chemical modification of surface components Biochim. biophys. Acta 66:237
    [Google Scholar]
  11. Hannan P. J. 1961; Electrophoretic properties of spores of Aspergillus niger. Appl. Microbiol 9:113
    [Google Scholar]
  12. Harold F. M., Miller A. 1961; Intracellular localization of inorganic polyphosphate in Neurospora crassa. Biochim. biophys. Acta 50:261
    [Google Scholar]
  13. Hartman R. S., Bateman J. B., Lauffer M. A. 1952; Electrophoresis by the microscope method: a simple experimental assembly. Archs Biochem. Biophys 39:56
    [Google Scholar]
  14. Hill M. J., James A. M., Maxted W. R. 1963; Some physical investigations of the behaviour of bacterial surfaces. IX. Studies on the streptococcal cell wall Biochim. biophys. Acta 75:402
    [Google Scholar]
  15. Ingram V. M., Salton M. R. J. 1957; The action of fiuorodinitrobenzene on bacterial cell walls. Biochim. biophys. Acta 24:9
    [Google Scholar]
  16. James A. M. 1965; Surface-active agents in microbiology. Surface Activity and the Microbial Cell p 3 S.C.I. Monograph no. 19. London;
    [Google Scholar]
  17. Kinsky S. C. 1962; Effect of polyene antibiotics on protoplasts of Neurospora crassa. J. Bact 83:351
    [Google Scholar]
  18. McQuillen K. 1950; The bacterial surface. I. Effect of cety 1-trimethyl-ammonium bromide on the electrophoretic mobility of certain Gram-positive bacteria. Biochim. biophys. Acta 5:463
    [Google Scholar]
  19. Phaff H. J. 1963; Cell wall of yeasts. A. Rev. Microbiol 17:15
    [Google Scholar]
  20. Richmond D. V., Somers E. 1962; Studies on the fungitoxidty of captan. I. The structural specificity of captan and six Af-trichloromethylthio analogues Ann. appl. Biol 50:33
    [Google Scholar]
  21. Somers E. 1966; The sites of reaction of fungicides in spores. In The Fungus Spore Ed. by Madelin M. F. p 299 London: Butterworths;
    [Google Scholar]
  22. Somers E., Pring R. J. 1966; Uptake and binding of dodine acetate by fungal spores. Arm. appl. Biol 58:457
    [Google Scholar]
  23. Villanueva J. R. 1966; Protoplasts of fungi. In The Fungi Ed. by Ainsworth G. C., Sussman A. S. 2 p 3 New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-48-1-147
Loading
/content/journal/micro/10.1099/00221287-48-1-147
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error